The effect of mobile phase modifiers on the simultaneous LC elution of a gadolinium complex and free ligand

Chellquist, Eric M.; Dicken, C. M.

doi:10.1016/0731-7085(93)80134-m

Cited by 9 publications

(3 citation statements)

References 3 publications

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“…To not only determine sum concentrations of GdCAs, separation techniques such as high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE) were applied for analysis of particular Gd complexes. HPLC methods include application of reversed-phase (RP) stationary phases, − ion-pair chromatography (IPC), − ion-exchange chromatography (IEC), , size-exclusion chromatography (SEC), and hydrophilic interaction chromatography (HILIC) . Detection of the separated GdCAs was performed by either UV−vis absorption, ,− , fluorescence, ,, radiometric, , ICP-MS, , or electrospray ionization (ESI)-MS , detection.…”

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confidence: 99%

Analysis of the Contrast Agent Magnevist and Its Transmetalation Products in Blood Plasma by Capillary Electrophoresis/Electrospray Ionization Time-of-Flight Mass Spectrometry

et al. 2009

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To study transmetalation effects of the gadolinium-based contrast agent Magnevist (Gd-DTPA), the first analytical method for the simultaneous determination of Gd-DTPA and its transmetalation products in complex clinical samples was developed. The high separation efficiency of capillary electrophoresis (CE) was employed to separate Gd-DTPA, Fe-DTPA, Cu-DTPA, Zn-DTPA, and the free DTPA (diethylenetriaminepentaacetic acid) ligand. The coupling of CE with electrospray ionization time-of-flight mass spectrometry (ESI-TOF-MS) provided the required sensitivity and excellent selectivity for the analysis of complex samples, such as blood plasma and whole blood. Separation and detection parameters were optimized, and crucial steps for CE/MS method development are pointed out. Limit of detection (LOD) is 5 x 10(-7) mol/L, limit of quantification (LOQ) is 1.7 x 10(-6) mol/L, and the linear range comprises 2 decades, starting at the limit of quantification. To determine recovery rates, precision, and accuracy of the method, blank plasma samples were spiked with Gd-DTPA in three different concentrations. Blood plasma samples from 10 patients with normal renal function, having received Magnevist, were analyzed for Gd-DTPA and possible transmetalation products by CE/ESI-TOF-MS. The method was validated by determination of the total Gd concentration using inductively coupled plasma optical emission spectroscopy (ICP-OES). Transmetalation assays of Magnevist with and without supplementary iron were carried out in incubated whole blood samples.

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Analysis of the Contrast Agent Magnevist and Its Transmetalation Products in Blood Plasma by Capillary Electrophoresis/Electrospray Ionization Time-of-Flight Mass Spectrometry

et al. 2009

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“…The use of capillary electrophoresis (CE) for the investigation of single contrast agents has been described by Vetterlein 19,20 and Campa. 21 Bettmer determined Gd-DTPA and Gd 3+ by coupling size exclusion chromatography (SEC) to ICP-MS. 22 Vora and Chellquist applied ion exchange chromatography (IEC) 23 and ion pair chromatography (IPC) 24 with UV-vis detection. HPLC methods based on reversed phase (RP) chromatography were introduced by Tweedle, Hvattum, Behra-Miellet, and Arbughi.…”

mentioning

confidence: 99%

“…Bettmer determined Gd-DTPA and Gd 3+ by coupling size exclusion chromatography (SEC) to ICP-MS . Vora and Chellquist applied ion exchange chromatography (IEC) and ion pair chromatography (IPC) with UV−vis detection. HPLC methods based on reversed phase (RP) chromatography were introduced by Tweedle, Hvattum, Behra-Miellet, and Arbughi. − Despite the great variety of analytical techniques, the majority of the presented methods exhibit certain limitations or drawbacks.…”

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Speciation Analysis of Gadolinium-Based MRI Contrast Agents in Blood Plasma by Hydrophilic Interaction Chromatography/Electrospray Mass Spectrometry

et al. 2008

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The first analytical method for simultaneous speciation analysis of five of the most important gadolinium-based magnetic resonance imaging (MRI) contrast agents in blood plasma samples was developed. Gd-DTPA (Magnevist), Gd-BT-DO3A (Gadovist), Gd-DOTA (Dotarem), Gd-DTPA-BMA (Omniscan), and Gd-BOPTA (Multihance) were separated by hydrophilic interaction liquid chromatography (HILIC) and detected with electrospray mass spectrometry (ESI-MS). Spiking experiments of blank plasma with Magnevist and Gadovist were performed to determine the analytical figures of merit and the recovery rates. The limits of detection ranged from 1 x 10 (-7) to 1 x 10 (-6) mol/L depending on the ionization properties of the individual compounds, and limits of quantification ranged from 5 x 10 (-7) to 5 x 10 (-6) mol/L. The linear concentration range comprised 2 orders of magnitude. With application of this method, blood plasma samples of 10 healthy volunteers, with Magnevist or Gadovist medication, were analyzed for Gd-DTPA and Gd-BT-DO3A, respectively. The obtained results were successfully validated with inductively coupled plasma-optical emission spectroscopy (ICP-OES).

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Handbook of Ion Chromatography

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The effect of mobile phase modifiers on the simultaneous LC elution of a gadolinium complex and free ligand

Cited by 9 publications

References 3 publications

Analysis of the Contrast Agent Magnevist and Its Transmetalation Products in Blood Plasma by Capillary Electrophoresis/Electrospray Ionization Time-of-Flight Mass Spectrometry

Analysis of the Contrast Agent Magnevist and Its Transmetalation Products in Blood Plasma by Capillary Electrophoresis/Electrospray Ionization Time-of-Flight Mass Spectrometry

Speciation Analysis of Gadolinium-Based MRI Contrast Agents in Blood Plasma by Hydrophilic Interaction Chromatography/Electrospray Mass Spectrometry

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