The Effect of MMP Inhibitor Metastat on Fissure Caries Progression in Rats

Tjäderhane, Leo; Sulkala, Merja; Sorsa, Timo; Teronen, Olli; Larmas, Markku; Salo, Tuula

doi:10.1111/j.1749-6632.1999.tb07762.x

Cited by 30 publications

(37 citation statements)

References 12 publications

(14 reference statements)

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“…This may be especially true in deep and active caries, due to the pulpal origin of cysteine cathepsins in caries lesions. MMP inhibition has been implicated in decreased caries lesion progression in dentin (Tjäderhane et al, 1999(Tjäderhane et al, , 2006Sulkala et al, 2001). Since MMPs and cysteine cathepsins may well have synergistic and adjunctive effects on caries pathogenesis, further research is needed to see if cysteine cathepsin inhibitors could also be used as therapeutic modes to retard or inhibit caries progression.…”

Section: Discussionmentioning

confidence: 99%

Cysteine Cathepsins in Human Carious Dentin

et al. 2011

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A supplemental appendix to this article is published electronically only at http://jdr.sagepub.com/supplemental. ABSTRACTMatrix metalloproteinases (MMPs) are important in dentinal caries, and analysis of recent data demonstrates the presence of other collagen-degrading enzymes, cysteine cathepsins, in human dentin. This study aimed to examine the presence, source, and activity of cysteine cathepsins in human caries. Cathepsin B was detected with immunostaining. Saliva and dentin cysteine cathepsin and MMP activities on caries lesions were analyzed spectrofluorometrically. Immunostaining demonstrated stronger cathepsins B in carious than in healthy dentin. In carious dentin, cysteine cathepsin activity increased with increasing depth and age in chronic lesions, but decreased with age in active lesions. MMP activity decreased with age in both active and chronic lesions. Salivary MMP activities were higher in patients with active than chronic lesions and with increasing lesion depth, while cysteine cathepsin activities showed no differences. The results indicate that, along with MMPs, cysteine cathepsins are important, especially in active and deep caries.

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Section: Discussionmentioning

confidence: 99%

Cysteine Cathepsins in Human Carious Dentin

et al. 2011

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“…68 Since Pashley et al 37 and Armstrong et al 39 auspiciously linked such degradation to the action of host derived endogenous matrix metalloproteinases (MMPs), 69 several approaches to make them inactive, hence slowing down or nullifying the phenomenon, started to be considered. 40,68,70,71,72 It is well-known that certain substances are promising MMPs inhibitors, but the most studied in adhesive dentistry is chlorhexidine (CHX). 37,58,73,74 It is, in addition, also capable of inhibiting cysteine cathepsins (CCs) 75 the other key class of proteolytic enzymes identified in dentin.…”

Section: Enzymatic Inhibitorsmentioning

confidence: 99%

“…Inhibitors of endogenous dentin proteases other than CHX, such as the quaternary ammonium surface-acting benzalkonium chloride (BAC) 124,125,126,127 the tetracyclines (TCs) and their antimicrobially inactive analogs (minocycline, for now), 71,84,128,129,130 bisphosphonates (batimastat, galardin, and zoled ron ate), 93,129,131,132 g reen tea poly phenol epigallocatechin-3-gallate (EGCG), 40,133,134 and the chelating agent ethylenediaminetetraacetic acid (EDTA) 135,136,137 are being studied as better alternatives associated with dentin hybrid layer preservation.…”

Section: Enzymatic Inhibitorsmentioning

confidence: 99%

Bonding efficiency and durability: current possibilities

et al. 2017

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Bonding plays a major role in dentistry nowadays. Dental adhesives are used in association with composites to solve many restorative issues. However, the wide variety of bonding agents currently available makes it difficult for clinicians to choose the best alternative in terms of material and technique, especially when different clinical situations are considered. Moreover, although bonding agents allow for a more conservative restorative approach, achieving a durable adhesive interface remains a matter of concern, and this mainly due to degradation of the bonding complex in the challenging oral environment. This review aims to present strategies that are being used or those still in development which may help to prevent degradation. It is fundamental that professionals are aware of these strategies to counteract degradation as much as possible. None of them are efficient to completely solve this problem, but they certainly represent reasonable alternatives to increase the lifetime of adhesive restorations.

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“…The specimens were then immersed in artificial saliva prepared in accordance with Pashley et al, 14 but without protease inhibitors, for the proposed aging times at 378C in a specially constructed large incubator, to accommodate the intrapulpal pressure assembly. Artificial saliva was composed of (mmol=L): CaCl 2 (0.7), MgCl 2 (0.6), H 2 O (0.2), KH 2 PO 4 (4.0), KCl (30), NaN 3 (0.3), and HEPES buffer (20). Sodium azide was added to prevent bacterial growth.…”

Section: Specimen Grouping and Restorative Proceduresmentioning

confidence: 99%

“…18 One strategy to prevent the host-derived proteases is the usage of MMP inhibitors. These include either specific ones such as galardin 19 or nonspecific ones like chemically-modified tetracyclines 20,21 and chlorhexidine (CHX). 14,22 The effectiveness of CHX, as an MMP inhibitor, on bond durability to normal dentin (ND) has been the most frequently investigated strategy compared to other MMP inhibitors.…”

Section: Introductionmentioning

confidence: 99%

Effect of Chlorhexidine Pretreatment on Bond Strength Durability of Caries–affected Dentin Over 2-Year Aging in Artificial Saliva and Under Simulated Intrapulpal Pressure

Mobarak¹

2011

Operative Dentistry

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Pretreatment of caries-affected dentin with 5% chlorhexidine decelerates the bond strength loss over the long term. SUMMARYObjective: To evaluate the influence of 2% and 5% chlorhexidine (CHX) pretreatment on bond durability of a self-etching adhesive to normal (ND) and caries-affected (AD) dentin after 2-years of aging in artificial saliva and under simulated intrapulpal pressure (IPP).Methods: One hundred twenty freshly extracted carious teeth were ground to expose ND and AD. Specimens were distributed into three equal groups (n¼40) according to whether the dentin substrates were pretreated with 2% or 5% CHX or with water (control). Clearfil SE Bond (Kuraray) was applied to both substrates and composite cylinders (0.9 mm diameter and 0.7 mm height) were formed. Pretreatment and bonding were done while the specimens were subjected to 15 mm Hg IPP. After curing, specimens were aged in artificial saliva at 378C and under IPP at 20 mm Hg until being tested after 24 hours or 2 years (n¼20/group). Microshear bond strength was evaluated. Failure modes were determined using a scanning electron microscope (SEM) at 4003 magnification. Data were statistically analyzed using three-way analysis of variance (ANOVA); oneway ANOVA tests, and t-test (p,0.05). Additional specimens (n¼5/group) were prepared to evaluate interfacial silver precipitation.Results: For the 24-hour groups, there were no significant differences among the ND groups and AD groups. For ND aged specimens, the 5% CHX group had the highest value followed by the 2% CHX and control groups, although the difference was statistically insignificant. For AD aged specimens, the 5% CHX group revealed statistically higher bond values compared to the 2% CHX and control groups. Fracture modes were predominately adhesive and mixed. Different interfacial silver depositions were recorded.Conclusions: Two percent or 5% CHX pretreatment has no adverse effect on the 24-hour bonding to ND and AD. Five percent CHX was able to diminish the loss in bonding to AD after 2years of aging in artificial saliva and under simulated IPP.

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The Effect of MMP Inhibitor Metastat on Fissure Caries Progression in Rats

Cited by 30 publications

References 12 publications

Cysteine Cathepsins in Human Carious Dentin

Cysteine Cathepsins in Human Carious Dentin

Bonding efficiency and durability: current possibilities

Effect of Chlorhexidine Pretreatment on Bond Strength Durability of Caries–affected Dentin Over 2-Year Aging in Artificial Saliva and Under Simulated Intrapulpal Pressure

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