The Effect of Minocycline on MMP-9 Levels in Traumatic Brain Injury: An Experimental Study in Wistar Rats

Abstract

Introduction
 The matrix metalloproteinase-9 (MMP-9) inhibition is an important target in the treatment of traumatic brain injury. Recent studies have shown that Minocycline may have MMP-9 inhibition activity. This study aims to determine the effect of minocycline in inhibiting MMP-9 in cases of head injury which has not been studied much.
 
 Methods
 This research was conducted using a laboratory experimental method on rats with a post-test control group design. The research was conducted at the Animal Laboratory, Faculty of Medicine, Hasanuddin University for a period of 2 weeks. The research population was rats with traumatic brain injury and rats without traumatic brain injury as controls. The sample size for each group was determined with Federer's formula, the minimum number of samples for each group was 9 experimental animals (a total of 27 mice in this study). Group 1 are control group, group 2(TBI) are rats with a traumatic brain injury, and group 3 are rats with a traumatic brain injury who was given minocycline.
 
 Results
 The mean value of MMP-9 in group 2 (TBI) was 0.610116 ng/ml compared to the control mean value of 0.519300 ng/ml. The mean value of MMP-9 in group 3 (TBI+M) was 0.552674 ng/ml. From the ANOVA test, there was a significant relationship with a p-value = 0.001 There was a statistically significant relationship between the administration of minocycline and the decrease in MMP-9 levels in traumatic brain injury in Wistar rats.
 
 Conclusion
 The level of MMP-9 in Wistar rats with traumatic brain injury who were given minocycline was shown to be significantly lower than that of those without minocycline treatment. This result showed that minocycline has the potential to inhibit the increase of MMP-9 levels in traumatic brain injury.

The Effect of Doxycycline on Matrix Metalloproteinase-9 Levels in the Colon of Wistar Rats with Obstructive Ileus

“…Their study also showed that colonic obstruction for four days increased collagen synthesis, which was positively correlated with obstruction time and significantly higher (more than 2fold) in the obstructed than in the non-obstructed left colon wall. This finding is consistent with Hayashi et al 17 and Perdana et al 18 , who showed that MMP-9 levels increased significantly at 24 and 48 hours after treatment of traumatic brain injury in experimental rats.…”