The effect of mimicking febrile temperature and drug stress on malarial development

Abstract

Background: Malaria remains one of the most important tropical diseases of human with 1-2 million deaths annually especially caused by P. falciparum. During malarial life cycle, they exposed to many environmentally stresses including wide temperature fluctuation and pharmacological active molecules. These trigger malarial evolutionarily adaptive responses. The effect of febrile temperature on malarial growth, development and drug susceptibility by mimicking patient in treatment failure before and after drug uptake was examined.

A Novel Flow Cytometric Hemozoin Detection Assay for Real-Time Sensitivity Testing of Plasmodium falciparum

“…However, slightly higher values were observed for some of the drugs, especially for artesunate, where most publications report values below 2.5 nM [15], [37], [47]. One explanation could be the inoculum effect, i.e., an increase in the inhibitory drug concentration when greater numbers of parasites are inoculated.…”

Single-Cell RNA Sequencing Reveals Cellular Heterogeneity and Stage Transition under Temperature Stress in Synchronized Plasmodium falciparum Cells

“…Therefore, in this study, we carefully selected the temperature (40°C), the time point (6 h of treatment), and the stage of the parasite (late-ring to early trophozoite stage), which have a minimal effect on parasite survival ( Fig. 1A ) ( 20 , 37 , 38 ). Moreover, we monitored the growth of the parasites using Giemsa stain upon temperature treatment, and we did not observe any significant change in the parasitemia after the 6-h temperature treatment as well in the following cycle (15 to 16% parasitemia) of IDC.…”