The effect of methyl jasmonate on triterpene and sterol metabolisms of Centella asiatica, Ruscus aculeatus and Galphimia glauca cultured plants

Mangas, Susana; Bonfill, Mercedes; Osuna, Lidia; Moyano, Encarnación; Tortoriello, Jaime; Cusidó, Rosa M.; Piñol, M. Teresa; Palazón, Javier

doi:10.1016/j.phytochem.2006.06.025

Cited by 101 publications

(67 citation statements)

References 26 publications

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“…However, several studies showed contradictory results on phytosterols accumulation after MeJA elicitation in different plants or plant cell cultures. For example, phytosterols production was enhanced in Lemna paucicostata cultured plants (Suh et al 2013) and Capsicum annuum cell culture (Sabater-Jara et al 2010), decreased in Centella asiatica and Ruscus aculeatus cultured plants (Mangas et al 2006), and remained constant in Galphimia glauca cultured plants (Mangas et al 2006) and G. glabra cell cultures (Hayashi et al 2003). The different results may indicate the existence of different control mechanisms among the plant species.…”

Section: Analysis Of Phytosterolsmentioning

confidence: 99%

Metabolic alterations and distribution of five-carbon precursors in jasmonic acid-elicited Catharanthus roseus cell suspension cultures

Saiman

Mustafa

Choi

et al. 2015

Plant Cell Tiss Organ Cult

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In Catharanthus roseus cell cultures, the terpenoid moiety is considered as the limiting factor in terpenoid indole alkaloid (TIA) production. The pathway of terpenoidal precursors in TIA is strongly linked with other terpenoid pathways, suggesting that TIA production might be limited by competition for the five-carbon (C5) precursors. This raises the question whether the stimulation of TIA production by certain signal molecules is due to a redistribution of C5 precursors between the associated terpenoid pathways and/or to a total increase of the precursor availability. To investigate the effect of a TIA-increasing signal molecule on C5 distribution, the cell suspension cultures of C. roseus were elicited with jasmonic acid (JA) and the metabolic changes of different terpenoid pathways were evaluated targeting on TIA (monoterpenoid; C10), carotenoids (tetraterpenoid; C40), and phytosterols (triterpenoid; C30). The chromatographic analyses showed that TIA and carotenoid levels almost doubled upon JA elicitation, while phytosterol levels remained constant if compared to the controls. Apparently, both TIA and carotenoid routes benefit from an increased C5 flow in the methyl-erythritol phosphate pathway, and potential export of C5 precursors to the cytosolic terpenoid routes, e.g., towards the phytosterols is minimal. However, the relative composition of individual compounds within each group remains similar when comparing elicited and control cells. This suggests that the increased production of TIA and carotenoid upon JA elicitation is predominantly caused by an increase of the precursor availability rather than due to a redistribution of C5 precursors between the associated terpenoid pathways. Furthermore, NMR-based metabolomics analysis showed a discrimination of JAelicited and control cells between 24 and 72 h after treatments with significant changes in levels of strictosidine, malic acid, and sucrose. This study portrays metabolic alterations upon JA elicitation and channeling of C5 precursors in different terpenoid biosynthetic pathways, which provides a knowledge platform for developing strategies to engineer fluxes in a complex biosynthetic network in order to obtain high TIA-producing C. roseus cell lines.

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Section: Analysis Of Phytosterolsmentioning

confidence: 99%

Metabolic alterations and distribution of five-carbon precursors in jasmonic acid-elicited Catharanthus roseus cell suspension cultures

Saiman

Mustafa

Choi

et al. 2015

Plant Cell Tiss Organ Cult

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“…13,14) Especially, there were many reports concerning induction by plant hormones, jasmonic acid, and methyl jasmonate (MeJA). In many cases, MeJA enhanced the production of pharmacologically important secondary metabolites such as defense compounds, phytoalexins, [15][16][17] and defence systems in plants. 18) We reported the production of a coumarin-type phytoalexin, umbelliferone, in Pharbitsis nil hairy roots.…”

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confidence: 99%

Production of Sesquiterpene-Type Phytoalexins by Hairy Roots of Hyoscyamus albus Co-treated with Cupper Sulfate and Methyl Jasmonate

Kawauchi

Arima

Shirota

et al. 2010

CHEMICAL & PHARMACEUTICAL BULLETIN

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The gram-negative bacteria Agrobacterium rhizogenes is a plant pathogen. On infecting plants it induces adventitious roots, known as "hairy roots." Hairy roots have an excellent growth capacity in simple culture systems without requiring the addition of any exogenous plant hormones.1,2) Furthermore, hairy roots are known to produce secondary metabolites at a high yield compared to those of undifferentiated plant cell suspensions and their mother plants. So, hairy roots were expected to provide a culture system for the production of pharmacologically important natural products and the biotransformation of organic compounds into useful biologically active compounds. Thus, many medicinal plants have been transformed to hairy roots, and there have been many related reports on the efficient production of useful natural products 3-7) and biotransformation of organic substances into useful compounds.8-10) Furthermore, hairy roots were expected to become a simple and easy experimental system for the biosynthesis of secondary metabolites such as phytoalexins, and plant gene engineering.11,12) Elicitation of hairy roots on culture with heavy metal ions such as cupper ion, organic chemicals such as jasmonic acid and biological substances such as yeast extract was studied, and found to enhance defense processes in plants and the production of secondary metabolites such as phytoalexins. 13,14) Especially, there were many reports concerning induction by plant hormones, jasmonic acid, and methyl jasmonate (MeJA). In many cases, MeJA enhanced the production of pharmacologically important secondary metabolites such as defense compounds, phytoalexins, [15][16][17] and defence systems in plants. 18) We reported the production of a coumarin-type phytoalexin, umbelliferone, in Pharbitsis nil hairy roots.19) The P. nil hairy roots also showed substrate specific and strong reactivity on the glucosylation of low-molecular-weight phenolic compounds, and strong reactivity on the reduction of a formyl group on the aromatic ring to a hydroxymethyl group. 20,21) Solanaceae, such as Solanum, Hyoscyamus, and Atropa sp., were used as medicinal plants, yielding alkaloids such as nicotine and tropan alkaloids as the main constituents, but they produced sesquiterpene-type phytoalexins, such as lubimine 22) and solavetivone, 23) after treatment with biotic and abiotic elicitors. We reported the production of sesquiterpene-type phytoallexins in Hyoscyamus albus (Solanaceae) hairy roots treated with MeJA,24) and the efficient production of tropane alkaloids in H. niger hairy roots. 25) We also reported the identification of a strongly expressed gene in H. niger hairy roots, related to and enhancing lateral root formation in H. niger hairy roots. 26) We previously found that products from H. albus hairy roots treated with CuSO 4 , MeJA, and co-treatment of CuSO 4 -MeJA showed different production patterns in terms of the TLC profile. So, we attempted to produce sesquiterpene-type phytoallexins in H. albus hairy roots cell culture by co-treatment with...

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“…A number of reports have been published on the relationship between MeJA and the terpene metabolism (Mangas et al 2006;van Schie et al 2007). In Picea abies, the MeJA treatment enhanced the productions of the mono-and diterpenoids, as well as their respective terpene synthase activities, in the wood (Martin et al 2002;Bohlmann 2006a, 2006b), implying that the transcriptions of the genes responsible for the resin acid biosynthesis were enhanced.…”

Section: Discussionmentioning

confidence: 99%

Two copies of 4-(cytidine 5′-diphospho)-2-C-methyl-d-erythritol kinase (CMK) gene in Ginkgo biloba: molecular cloning and functional characterization

Kim

Kuzuyama

et al. 2008

Planta

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4-(Cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (CMK or YchB), the fourth enzyme of the 2-C-methyl-D-erythritol 4-phosphate pathway, phosphorylates the 2-hydroxyl group of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol in the presence of ATP. Two isogenes encoding CMK (GbCMK1 and GbCMK2) were cloned and characterized from Ginkgo biloba. The activities of both isozymes were confirmed by complementation assay using Escherichia coli NMW29, a ychB knock-out mutant. The transcript profiles of GbCMKs in the radicles and the cotyledons of the cultured Ginkgo biloba embryos demonstrated that the transcript levels of GbCMK1 were similar in both organs, whereas that of GbCMK2 was predominantly high in the ginkgolide-synthesizing radicles. Selective increases in the transcript abundance of GbCMK2 in the radicles, induced by light and methyl jasmonate treatments, were observed. These differential induction patterns of the transcripts imply GbCMK1 and GbCMK2 respectively have high correlations with the primary and the secondary metabolisms. The transit peptides of both isozymes delivered the fused green fluorescent protein (GFP) into the chloroplast in the Arabidopsis and the Nicotiana transient expression systems; interestingly, the transit peptide of GbCMK1 delivered the GFP protein into the cytosol and the nucleus in addition to the chloroplasts.

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The effect of methyl jasmonate on triterpene and sterol metabolisms of Centella asiatica, Ruscus aculeatus and Galphimia glauca cultured plants

Cited by 101 publications

References 26 publications

Metabolic alterations and distribution of five-carbon precursors in jasmonic acid-elicited Catharanthus roseus cell suspension cultures

Metabolic alterations and distribution of five-carbon precursors in jasmonic acid-elicited Catharanthus roseus cell suspension cultures

Production of Sesquiterpene-Type Phytoalexins by Hairy Roots of Hyoscyamus albus Co-treated with Cupper Sulfate and Methyl Jasmonate

Two copies of 4-(cytidine 5′-diphospho)-2-C-methyl-d-erythritol kinase (CMK) gene in Ginkgo biloba: molecular cloning and functional characterization

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