The effect of methanol rhizome extract of Nymphaea lotus Linn. (Nymphaeaceae) in animal models of diarrhoea

Bello, Fatima; Maiha, Bilkisu Bello; Anuka, JA

doi:10.1016/j.jep.2016.05.036

Cited by 36 publications

(29 citation statements)

References 29 publications

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“…To restore personal comfort and convenience, many patients require anti-diarrheal therapy and treatment is carried out to achieve ( Bello et al, 2016 ). Dihuang powder is used as folk formulation for treating diarrhea, and is composed of six herbal medicines.…”

Section: Discussionmentioning

confidence: 99%

The Anti-diarrheal Activity of the Non-toxic Dihuang Powder in Mice

Shang¹,

Miao²,

Yang³

et al. 2018

Front. Pharmacol.

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Dihuang powder (DHP) has been used in the traditional Chinese medicine for the treatment of diarrhea in some regions of China. But up to now, the anti-diarrheal activity of DHP haven’t been performed with modern pharmacological technology. This study aims to investigate the quality control, the potential toxicity and anti-diarrheal activity of Dihuang powder in mice. High performance liquid chromatography (HPLC) and thin layer chromatography (TLC) were used to detect five active compounds in DHP for quality control, and the acute toxicity and sub-acute toxicity for 28-day oral administration of DHP were then evaluated. The anti-diarrheal activity was investigated using mouse model. Results showed that the levels of quercetin and berberine in DHP were 0.054 and 0.632 mg/g, respectively, and atractylodin, matrine, and patehouli aleohal were also detected in DHP. At the given doses, DHP was safe in terms of acute and sub-acute toxicity. Meanwhile, DHP exhibited strong anti-diarrheal effects as well as decreased gastrointestinal motility and the secretions induced by Sennae and castor oil in a dose-dependent manner. It could decrease the content of IL-1β, IL-6, and TNF-α in the small intestine, and improve the histopathological changes of small intestine and large intestine induced by Sennae. The antinociceptive and anti-inflammatory activities in vivo also were presented. Based on all of the results, we thought that DHP has anti-diarrheal activity, and could be used to treat diarrhea as well as alleviate the pain and inflammation induced by diarrhea. This study provides a theoretical basis for the clinical use of DHP and may assist in the development of new drugs for the treatment of diarrhea. The mechanism of the anti-diarrheal activity should be investigated in the future.

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Section: Discussionmentioning

confidence: 99%

The Anti-diarrheal Activity of the Non-toxic Dihuang Powder in Mice

Shang¹,

Miao²,

Yang³

et al. 2018

Front. Pharmacol.

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“…Due to the beauty of its flower, this plant is widely used and cultivated as an ornamental plant. Moreover, N. lotus is used as a medicinal plant for traditional medicines, food and herbal tea for health benefits, especially in many Asian countries, Egypt and other countries in Africa [ 1 , 2 , 3 , 4 , 5 ]. Almost every part of this medicinal plant is edible.…”

Section: Introductionmentioning

confidence: 99%

“…This study indicated that the N. lotus flowers extract probably account for neuroprotective, immune-boosting and antioxidant activity without neurotoxicity [ 10 ]. Furthermore, the pharmacological and medicinal potential of this medicinal species were currently confirmed at in vivo level, i.e., anti-diarrhea effect of N. lotus rhizome extract [ 5 ], anxiolytic and antidepressant potential from the N. lotus leaf extract [ 11 ]. Oyeyemi et al [ 12 ] investigated the hepatoprotective effect of N. lotus methanol extract to fight against carbon tetrachloride-induced chronic hepatotoxicity in vivo model using Wistar rats.…”

Section: Introductionmentioning

confidence: 99%

Enrichment in Antioxidant Flavonoids of Stamen Extracts from Nymphaea lotus L. Using Ultrasonic-Assisted Extraction and Macroporous Resin Adsorption

Tungmunnithum

Drouet

Kabra³

et al. 2020

Antioxidants

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Nymphaea lotus L. is the medicinal plant that has long been used for food, cosmetics and traditional medicines in Africa and Asia since ancient times. Its flavonoids and other interesting phytochemical compounds from rhizome, leaf and the whole flowers have been reported in the previous published research. However, stamens, which are essential for reproductive functions, may also represent new alternative sources of potential antioxidant flavonoids, as investigated in this study. The innovative green chemistry methods, i.e., ultrasound-assisted extraction (UAE) as well as a macroporous resin (MPR) purification procedure, were employed in this current research. Using a full factorial design coupled to three-dimensional (3D) surface plot methodology, the influence of three variables, namely aqEtOH concentration (ranging from 50 to 100% (v/v), US frequency (ranging from 0 (no US applied) to 45 kHz), and the extraction duration (ranging from 20 to 60 min), were evaluated. Five MPRs with different surface areas, average pore diameters, matrix types and polarities were also investigated for the purification of total flavonoids. The optimal UAE condition is 90% (v/v) aqEtOH with 34.65 khz ultrasonic frequency and 46 min of extraction duration. Compared with the conventional heat reflux extraction (HRE) method, a significant 1.35-fold increase in total flavonoids content was obtained using optimized UAE conditions (169.64 for HRE vs. 235.45 mg/g dry weight for UAE), causing a 2.80-fold increase when this UAE associated with MPR purification (475.42 mg/g dry weight). In vitro cell free antioxidant activity of N. lotus stamen extracts and in cellulo antioxidant investigation using yeast model showed the same trend, indicating that the best antioxidant flavonoid can be found in UAE coupled with MPR purification. Moreover, in the yeast model, the expression of key antioxidant genes such as SIR2 and SOD2 were expressed at the highest level in yeast cells treated with the extract from UAE together with MPR purification. Consequently, it can be seen that the UAE combined with MPR purification can help enhance the flavonoid antioxidant potential of the stamens extract from this medicinal species.

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“…One experimental study on trypanocidal activity of N. lotus methanol extract against Trypanosoma brucei brucei demonstrated the potency of its crude extract in treating experimental trypanosomiasis at the dose of 100 mg/ kg/day [24]. In 2016, an animal study was conducted to evaluate the effects the antidiarrhoeal activity of the methanol rhizome extract of N. lotus plant in laboratory animals [25]. Results of this study revealed that the extract at the doses of 200, 400 and 800 mg/kg produced significant reduction in the frequency of diarrhea.…”

Section: Modern Applications Of Bioequivalence and Bioavailabilitymentioning

confidence: 99%

A Review of the Pharmacological potential of the Water Lilynymphaealotus

Mireille¹

2017

MABB

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Natural Product serve as a major source of drugs for centuries, and about half of the pharmaceuticals in use today are derived from Natural Product. Herbal medicines have become a popular form of therapy both in the developed and developing countries. However, many of these natural products are used by population despite the fact that the scientific data on their pharmaco toxic profile are unknown. In regard to this present trend, extracts from a medicinal plant widely spread in Africa, Nymphaea lotus L were the investigated natural material. This review summarizes thus the evidence for pharmacological activities of Nymphaea lotus Linn extracts, highlighting, where investigated, its pharmacotoxic potential.

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The effect of methanol rhizome extract of Nymphaea lotus Linn. (Nymphaeaceae) in animal models of diarrhoea

Cited by 36 publications

References 29 publications

The Anti-diarrheal Activity of the Non-toxic Dihuang Powder in Mice

The Anti-diarrheal Activity of the Non-toxic Dihuang Powder in Mice

Enrichment in Antioxidant Flavonoids of Stamen Extracts from Nymphaea lotus L. Using Ultrasonic-Assisted Extraction and Macroporous Resin Adsorption

A Review of the Pharmacological potential of the Water Lilynymphaealotus

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