The Effect of Metformin on Alveolar Bone in Ligature‐Induced Periodontitis in Rats: A Pilot Study

Bak, Eun‐Jung; Park, Hong Gyu; Kim, Minyoung; Kim, Sung Whan; Kim, Sungwuk; Choi, Seong Ho; Heon, Jeong; Yoo, Yun‐Jung

doi:10.1902/jop.2009.090414

Cited by 58 publications

(80 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Metformin administration significantly inhibited the progress of alveolar bone loss in the experimental groups after 28 days. These results confirm that of a recent study in which metformin was shown to directly inhibit bone loss in OVX rats and in rats with periodontitis (8,9). Enzyme histochemical observations indicated that the number of multinucleated osteoclasts in the periapical area in rats treated with metformin decreased at day 14.…”

Section: Discussionsupporting

confidence: 90%

“…Metformin significantly increases the alkaline phosphatase (ALP) activity and osteocalcin (OC) production and induces 2 key transcription factors for osteoblast differentiation (7). It also ameliorates bone loss in ovariectomized (OVX) rats (by regulating the activity of bone marrow cells) (8) and in ligature-induced periodontitis by promoting osteoblast differentiation (9).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Protective Effect of Metformin on Periapical Lesions in Rats by Decreasing the Ratio of Receptor Activator of Nuclear Factor Kappa B Ligand/Osteoprotegerin

Liu

Zhang

et al. 2012

Journal of Endodontics

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 90%

mentioning

confidence: 99%

Protective Effect of Metformin on Periapical Lesions in Rats by Decreasing the Ratio of Receptor Activator of Nuclear Factor Kappa B Ligand/Osteoprotegerin

Liu

Zhang

et al. 2012

Journal of Endodontics

View full text Add to dashboard Cite

“…The osteogenic effect of metformin on osteoblasts has been reported by several groups [Cortizo et al, 2006;Kanazawa et al, 2008;Bak et al, 2010]. But little is known about the effect of metformin on osteoclasts.…”

Section: Discussionmentioning

confidence: 98%

“…A recent report demonstrated that metformin prevented RANKL-induced osteoclasts differentiation in vitro [Gao et al, 2010]. However, study by Bak et al [2010] reported that metformin exerted no effects on osteoclast formation induced by VitD3, lipopolysaccharides, and prostaglandin E(2) (PGE 2 ). In this study, we confirmed that metformin inhibits osteoclast differentiation in a Raw264.7 cells and osteoblasts coculture system and in OVX rats.…”

Section: Discussionmentioning

confidence: 99%

Metformin stimulates osteoprotegerin and reduces RANKL expression in osteoblasts and ovariectomized rats

et al. 2011

View full text Add to dashboard Cite

Anti-diabetic drug metformin has been shown to enhance osteoblasts differentiation and inhibit osteoclast differentiation in vitro and prevent bone loss in ovariectomized (OVX) rats. But the mechanisms through which metformin regulates osteoclastogensis are not known. Osteoprotegerin (OPG) and receptor activator of nuclear factor κB ligand (RANKL) are cytokines predominantly secreted by osteoblasts and play critical roles in the differentiation and function of osteoclasts. In this study, we demonstrated that metformin dose-dependently stimulated OPG and reduced RANKL mRNA and protein expression in mouse calvarial osteoblasts and osteoblastic cell line MC3T3-E1. Inhibition of AMP-activated protein kinase (AMPK) and CaM kinase kinase (CaMKK), two targets of metformin, suppressed endogenous and metformin-induced OPG secretion in osteoblasts. Moreover, supernatant of osteoblasts treated with metformin reduced formation of tartrate resistant acid phosphatase (TRAP)-positive multi-nucleated cells in Raw264.7 cells. Most importantly, metformin significantly increased total body bone mineral density, prevented bone loss and decreased TRAP-positive cells in OVX rats proximal tibiae, accompanied with an increase of OPG and decrease of RANKL expression. These in vivo and in vitro studies suggest that metformin reduces RANKL and stimulates OPG expression in osteoblasts, further inhibits osteoclast differentiation and prevents bone loss in OVX rats.

show abstract

“…Foi demonstrado também que a MET possui efeitos benéficos no metabolismo ósseo nos pacientes diabéticos (Vestergaard et al, 2005;Renjmark, 2008). Bak et al (2010) avaliaram os efeitos da MET sistêmica no osso alveolar em ratos com periodontite induzida, e os resultados sugeriram que o medicamento pode reduzir o infiltrado inflamatório e a perda óssea alveolar nos tecidos periodontais.…”

Section: Resultados Demográficosunclassified

Comportamento dos sistemas peroxidase e antioxidante, e produtos de peroxidação lipídica na saliva total de pacientes com diabetes mellitus 2 antes e após tratamento periodontal não cirúrgico

Takahashi¹

View full text Add to dashboard Cite

Aos pacientes, muito obrigada pela participação e por confiarem neste estudo.Ao meu orientador Prof. Luiz Lima, primeiramente obrigada por ter me aceitado como orientada. Agradeço muito pela oportunidade de ter trabalhado ao seu lado, sempre aprendendo com seu conhecimento de altíssimo nível. Aprendi muito com a sua humildade, bondade e paciência para ensinar.Obrigada pelo apoio, por acreditar e confiar na minha capacidade.Ao meu orientador de iniciação científica Prof. Mário Taba Júnior da minha antiga casa FORP, quem me acolheu e me introduziu à periodontia. Saiba que o admiro muito e sou profundamente agradecida pelo que me ensinou.Ao Dr. Eduardo Sampaio e equipe de periodontia da APCD Vila Mariana. Agradeço por tudo que vocês me ensinaram, por terem feito parte da fase mais importante da minha carreira facilitando na minha decisão de seguir na área de periodontia.À Ana Paula Sassá Bendete... Sou eternamente grata a oportunidade que foi me dada em trabalhar com você. Aprendi muito com a sua paciência, inteligência e bondade.Obrigada pelo acolhimento que você e o prof. Luiz me proporcionaram nessa linha de pesquisa tão linda que acabei me apaixonando. Quando entrei na pesquisa não sabia que ia encontrar alguém que fosse ser importante na minha vida, uma AMIGA. Foi muito gratificante conhecer você e sua linda família!!! Ao meu namorado Willian N. Kaneko agradeço do fundo do coração pelas palavras de conforto nas horas de desabafo e tensão, e pelos conselhos durante toda essa jornada.Obrigada pela compreensão, pelo apoio e principalmente pela paciência nesses últimos anos que significaram tanto na minha vida. Te amo muito! Ao Rafael Takahashi, Letícia Kimura, Thainah, Rafaela e Caroline Kaneko Ishii, Kimiyo e Sérgio Kaneko. Obrigada por todo apoio e por terem tornado essa jornada mais leve e divertida. were submitted to NSPT, and those with periodontal health (DM and C) received oral hygiene instructions and prophilaxys only. Results: At baseline, DMPC and PC patients showed PD, BOP, PI and CAL higher levels when compared to DM and C groups (p<0.05). After NSPT, all these clinical parameters had significant improvement (p<0.05) in both groups. HbA1c medians were significantly higher in DMPC when compared to DM, at baseline, however, the NSPT did not improved after 1 and 3 months of glicemic control, though percentual reduction (0.4%) observed at clinical point of view might be considered important. As to laboratorial analysis, in DMPC group, TBARS levels were significantly decreased compared to DM, PC and C (p<0.05), and SPO activity were significantly higher than PC and C (p<0.05), with negative correlation between TBARS and SPO (r=-0.35; p=0.002), while TAS levels and SOD activity did not show any significant differences among studied groups (p>0.05). After NSPT, TBARS levels increased significantly, and SPO decreased significantly only in DMPC group (p<0.05 and p<0.05), whereas SOD and TAS values remained unchanged in DMPC and PC groups. Conclusion: At baseline, TBARS levels and SPO activity have been altere...

show abstract

The Effect of Metformin on Alveolar Bone in Ligature‐Induced Periodontitis in Rats: A Pilot Study

Abstract: Our findings suggest that metformin may exert a beneficial effect on alveolar bone in periodontitis by increasing osteoblast differentiation.

Cited by 58 publications

References 38 publications

Protective Effect of Metformin on Periapical Lesions in Rats by Decreasing the Ratio of Receptor Activator of Nuclear Factor Kappa B Ligand/Osteoprotegerin

Protective Effect of Metformin on Periapical Lesions in Rats by Decreasing the Ratio of Receptor Activator of Nuclear Factor Kappa B Ligand/Osteoprotegerin

Metformin stimulates osteoprotegerin and reduces RANKL expression in osteoblasts and ovariectomized rats

Comportamento dos sistemas peroxidase e antioxidante, e produtos de peroxidação lipídica na saliva total de pacientes com diabetes mellitus 2 antes e após tratamento periodontal não cirúrgico

Contact Info

Product

Resources

About