The effect of melatonin on lipid peroxidation during radiotherapy in female rats

Kaya, Hakan; Delibas, Namik; Serteser, Mustafa; Ulukaya, Engin; Özkaya, Okan

doi:10.1007/bf02743581

Cited by 92 publications

(60 citation statements)

References 14 publications

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“…Ionizing radiation has been reported to increase the plasma and ovarian TBARS levels in rats and melatonin administration significantly decreased these levels (30). The radioprotective effect of melatonin has been attributed to several mechanisms such as scavenging of free radicals, distribution throughout the organism, ability to induce the antioxidant enzymes, and high bioavailability (31).…”

Section: Discussionmentioning

confidence: 99%

Melatonin prevents inflammation and oxidative stress caused by abdominopelvic and total body irradiation of rat small intestine

Güney

Hiçsönmez

Uluoğlu

et al. 2007

Braz J Med Biol Res

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We investigated the day-night differences in intestinal oxidativeinjury and the inflammatory response following total body (TB) or abdominopelvic (AP) irradiation, and the influence of melatonin administration on tissue injury induced by radiation. Rats (male Wistar, weighing 220-280 g) in the irradiated groups were exposed to a dose of 8 Gy to the TB or AP region in the morning (resting period -1 h after light onset) or evening (activity span -13 h after light onset). Vehicle or melatonin was administered immediately before, immediately after and 24 h after irradiation (10, 2.0 and 10 mg/kg, ip, respectively) to the irradiated rats. AP (P < 0.05) and TB (P < 0.05) irradiation applied in the morning caused a significant increase in thiobarbituric acid reactive substance (TBARS) levels. Melatonin treatment in the morning (P < 0.05) or evening (P < 0.05) decreased TBARS levels after TB irradiation. After AP irradiation, melatonin treatment only in the morning caused a significant decrease in TBARS levels (P < 0.05). Although we have confirmed the development of inflammation after radiotherapy by histological findings, neither AP nor TB irradiation caused any marked changes in myeloperoxidase activity in the morning or evening. Our results indicate that oxidative damage is more prominent in rats receiving TB and AP irradiation in the morning and melatonin appears to have beneficial effects on oxidative damage irrespective of the time of administration. Increased neutrophil accumulation indicates that melatonin administration exerts a protective effect on AP irradiation-induced tissue oxidative injury, especially in the morning.

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Section: Discussionmentioning

confidence: 99%

Melatonin prevents inflammation and oxidative stress caused by abdominopelvic and total body irradiation of rat small intestine

Güney

Hiçsönmez

Uluoğlu

et al. 2007

Braz J Med Biol Res

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“…4 Lipid peroxidation is believed to be an important cause of destruction and damage to cell membranes and has been suggested to be a contributing factor to the development of oxygen radicals-mediated tissue damage. 5 Oxidative stress occurs when there is excessive free radical production and/or low antioxidant defence, and results in chemical alterations of biomolecules causing structural and functional modifications. 6 The generation of the reactive oxygen metabolites (ROMs) plays an important role in the pathogenesis of radiation-induced tissue injury.…”

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confidence: 99%

Aminoguanidine ameliorates radiation-induced oxidative lung damage in rats

Eroğlu¹,

Yildiz²,

Saraymen³

et al. 2008

CIM

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Clin Invest Med 2008; 31 (4): E182-E188.Abstract Purpose: To investigate the possible protective effects of aminoguanidine (AG ) on lung damage in whole body irradiated rats. Methods: To evaluate the biological damage of radiation on rat lung tissue, lipid peroxidation products were measured using biochemical parameters. Thirty Wistar albino rats were divided into three subgroups: control (C) , irradiation alone (RT), and RT + AG combined. After sacrificing the rats, antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSHPx) activities and malondiadehyde (MDA), nitric oxide (NO) levels were evaluated in lung tissue. Results: Administration of AG resulted in an increase in the activities of CAT, SOD and GSHPx in the lungs. All were reduced after radiatio. In addition, AG administration resulted in a decrease in both NO and MDA levels in lung compared with the irradiated group. Conclusion: Amnoguanidine increased the endogenous antioxidant defence mechanism in rats and protected the animals from radiation-induced lung toxicity. Moreover, AG may protect against ionizing radiation-induced lung damage because of its antioxidant effect.Radiation therapy (RT) is a crucial component of application for many thoracic malignancies. Radiationinduced lung toxicity remains a major factor that limits the ability to escalate radiation doses in the application of thoracic tumours. RT-related pulmonary symptoms occur in up to 30% of patients irradiated for lung cancer, breast cancer, lymphoma, or thymoma. Pulmonary toxicity from thoracic irradiation is also an important problem in other settings, including combined modality treatment of Hodgkin's disease and esophageal cancer, and bone marrow transplantation with total body irradiation. 1 Ionizing radiation is a well-established carcinogen due to the resulting oxidative damage, and the molecule most often reported to be damaged by this physical agent is DNA. 2 Aproximately 60-70% of cellular DNA damage produced by ionizing radiation is caused by OH, formed from the radiolysis of water. 3 Besides DNA, lipids and proteins are also attacked by free radicals induced by ionizing radiation. 4 Lipid peroxidation is believed to be an important cause of destruction and damage to cell membranes and has been suggested to be a contributing factor to the development of oxygen radicals-mediated tissue damage. 5 Oxidative stress occurs when there is excessive free radical production and/or low antioxidant defence, and results in chemical alterations of biomolecules causing structural and functional ORIGINAL RESEARCH

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“…Peroxynitrite formation is thought to be the reason for this elevation because peroxynitrite is recognized as an intermediate reactive species that participates in many injury processes associated with oxidative damage and causes more powerful peroxydation of unsaturated fatty acids in cellular membranes than other radicals (Radi et al, 1991;Hogg et al, 1992;Van der vliet et al, 1994). Kaya et al demonstrated that radiotherapy gives rise to significant exacerbation of TBARS levels in blood and over tissues (Kaya et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Exacerbative role of vitamın A on radiation damage in vivo

et al. 2008

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Purpose In our study, after applying a single dose of 612 cGy irradiation, we aimed to observe the role of free radicals on tissue damage in liver caused by irradiation, by measuring the nitric oxide (NO) level, superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzyme activities, and the amount of thiobarbituric acid reactive substance (TBARS), which is an indicator of free-radical damage. On the other hand we investigated whether tissue damage can be prevented by vitamin A or not. Materials and methods The study was performed on three groups: (1) the control group, (2) the group to which irradiation was administrated, and (3) the group which was given irradiation + vitamin A. The irradiation group of animals was given a single dose of gamma irradiation at a sublethal dose. In the group to which both irradiation and vitamin A were administered, vitamin A was given for 2 days prior to irradiation. The amount of NO was measured by electron spin resonance (ESR) spectroscopy, whereas SOD, GPx and TBARS were measured by spectrophotometry. Results and conclusion As a result of irradiationmediated tissue damage in liver, we observed a NO loss and an increase in TBARS amount. Administration of vitamin A before irradiation resulted in an increase in MEDICINAL CHEMISTRY RESEARCH both NO and TBARS and a decrease in SOD and GPx enzyme activities. Together, these data indicate that vitamin A may play an exacerbative role in free-radicalmediated tissue damage.

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The effect of melatonin on lipid peroxidation during radiotherapy in female rats

Abstract: Melatonin, in rats, reduced the level of MDA, which is elevated by radiotherapy and increased the levels of SOD and GPX, which are involved in the antioxidant system.

Cited by 92 publications

References 14 publications

Melatonin prevents inflammation and oxidative stress caused by abdominopelvic and total body irradiation of rat small intestine

Melatonin prevents inflammation and oxidative stress caused by abdominopelvic and total body irradiation of rat small intestine

Aminoguanidine ameliorates radiation-induced oxidative lung damage in rats

Exacerbative role of vitamın A on radiation damage in vivo

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