The Effect of Melatonin on Liver Superoxide Dismutase Activity, Serum Nitrate and Thyroid Hormone Levels.

Öztürk, Güler; Coşkun, Şule; Erbaş, Deniz; Hasanoğlu, Enver

doi:10.2170/jjphysiol.50.149

Cited by 60 publications

(17 citation statements)

References 26 publications

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“…As a result, melatonin application can effectively protect cells from oxidative stress . For example, melatonin administration increases the activity of superoxide dismutase ( SOD ) in mice . Supplement of melatonin promotes the expressions of Gpx4 and SOD1 in bovine embryo .…”

Section: Introductionmentioning

confidence: 99%

Melatonin protects porcine oocyte in vitro maturation from heat stress

Liu

Zhang

et al. 2015

Journal of Pineal Research

117

110

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Melatonin is a pleiotropic molecule which plays an important role in animal reproductive activities. Because of the increased global warming, the impact of heat stress (HS) on stockbreeding has become an inevitable issue to be solved. To investigate the potential effects of melatonin on the in vitro maturation of porcine oocyte under the HS, a HS model for porcine oocyte maturation has been used in this study and the different concentrations of melatonin (10(-6) -10(-9) m) were also tested for their protective effects on oocytes. The polar body rate, the index of the nuclear maturation of the oocytes, and the cleavage rate as well as the blastocyst rate were measured to evaluate the developmental competence of the oocytes after parthenogenetic activation (PA). The results showed that HS [in vitro maturation (IVM) 20-24 hr, 42°C] significantly reduced the polar body rate of oocytes and the blastocyte rate of porcine PA embryos, while melatonin (10(-7) m) application not only improved polar body rate and blastocyte rate, but also preserved the normal levels of steroid hormone which is disrupted by HS. The presence of melatonin (10(-7) m) during the oocyte maturation under the HS reduced reactive oxygen species (ROS) formation, enhanced glutathione (GSH) production, inhibited cell apoptosis, and increased the gene expressions of SIRT1, AKT2, and Polg2. Importantly, the endogenously occurring melatonin of cumulus-oocyte complexes was significantly induced by HS. The results indicated that melatonin application effectively protected the oocytes from HS. These observations warranted the further studies in vivo regarding to improve the reproductive activities of animals under the global warming environment.

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Section: Introductionmentioning

confidence: 99%

Melatonin protects porcine oocyte in vitro maturation from heat stress

Liu

Zhang

et al. 2015

Journal of Pineal Research

117

110

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“…Melatonin eliminates oxygen free radicals such as hydroxyl radical, singlet oxygen, hydrogen peroxide (H 2 O 2 ), peroxyl radical, and hypochlorous acid through its direct scavenging action [8][9][10][11][12][13][14][15]. In addition, melatonin reduces oxidative stress in normal rat livers by increasing the activities of antioxidant enzymes such as SOD, glutathione peroxidase (GSH-Px), and glutathione reductase (GSSG-R) and the level of an antioxidant GSH [16][17][18][19][20]. Melatonin also reduces oxygen free radical-induced damage in oxidatively damaged rat livers by maintaining or increasing the activities of antioxidant enzymes such as SOD, catalase, GSH-Px, and GSSG-R and the level of GSH [19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Preventive effect of melatonin on the progression of α‐naphthylisothiocyanate‐induced acute liver injury in rats

Ohta¹,

Kongo

Kishikawa

2003

Journal of Pineal Research

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: The preventive effect of melatonin on the progression of α‐naphthylisothiocyanate (ANIT)‐induced acute liver injury with cholestasis was examined in rats treated once with the hepatotoxin [75 mg/kg body weight (BW), i.p.]. In rats treated with ANIT alone, liver injury with cholestasis occurred 24 hr after treatment and progressed at 48 hr, judging from the serum levels of hepatobiliary marker enzymes and components. Melatonin (10 or 100 mg/kg BW) was orally administered to the ANIT‐treated rats, 24 hr after the hepatotoxin treatment at which time hepatic injury had already developed. The administered indoleamine prevented the progression of liver cell damage rather than biliary cell damage more effectively at the higher dose than at the lower dose. In rats treated with ANIT alone, the serum and hepatic concentrations of thiobarbituric acid reactive substances, an index of lipid peroxidation, and the hepatic activity of myeloperoxidase, an index of tissue neutrophil infiltration, increased 24 hr after treatment and further increased at 48 hr. In the liver of rats treated with ANIT alone, Cu,Zn‐superoxide dismutase activity decreased 24 hr after treatment and was further reduced at 48 hr, although there was no change in Mn‐superoxide dismutase activity. Catalase and Se‐glutathione peroxidase activities also decreased at 48 hr, while reduced glutathione concentrations remained increased at 24 and 48 hr. The melatonin administered to the ANIT‐treated rats attenuated the increases in serum and hepatic concentrations of thiobarbituric acid reactive substances and the decreases in hepatic activities of Cu,Zn‐superoxide dismutase, catalase, and Se‐glutathione peroxidase found at 48 hr after the hepatotoxin treatment more effectively at the higher dose than at the lower dose; on the other hand, melatonin treatment had no effect on the increases in hepatic myeloperoxidase activity and reduced glutathione concentration found at 48 h. These results indicate that orally administered melatonin at pharmacological doses prevents the progression of ANIT‐induced acute liver injury, mainly liver cell damage, in rats, and suggest that the administered melatonin exerts these preventive effects through its direct and indirect antioxidant actions.

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“…The exercise in this study was not as strenuous as previously described, but a similar decrease in concentration of T4 and fT4 as in strenuous exercise was seen [15]. Variables that could account for the decreases are multiple and could include exercise-induced alterations in distribution or metabolism of hormones [34], altered protein binding of thyroid hormones due to exposure to cold temperatures [35], changed melatonin production which is related to light and exercise [36,37] and diurnal fluctuation [38]. The contribution of the latter is considered to be minimal in this study, due to the similar collection times.…”

Section: Discussionmentioning

confidence: 63%

Exercise induced hypercoagulability, increased von Willebrand factor and decreased thyroid hormone concentrations in sled dogs

et al. 2014

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BackgroundSled dogs performing endurance races have been reported to have a high incidence of gastric erosions or ulcerations and an increased risk of gastro intestinal bleeding leading to death in some cases. In addition, these dogs also become hypothyroid during training and exercise. Canine hypothyroidism has been shown to correlate with decreased von Willebrand factor antigen and potentially increased bleeding tendency. Whether increased gastro intestinal bleeding risk is exacerbated due to changes in the hemostatic balance is unknown. The aim of this study was to investigate the hemostatic balance in sled dogs before and after exercise and in addition evaluate any correlation to thyroid status. Twenty sled dogs have been assessed in untrained and trained condition and immediately after exercise. The first sample was collected in the autumn following a resting period, and subsequently the dogs were exposed to increased intensity of training. After four months the peak of physical condition was reached and a 68 km long sled pulling exercise was performed. Samples were collected before and immediately after the exercise. Evaluated parameters were: plasma thromboelastographic (TEG) R, SP, α and MA, activated partial thromboplastin time (aPTT), prothrombin time (PT), fibrinogen, von Willebrand factor (vWf), D-dimer, platelet number, thyroid hormones, hematocrit and C-reactive protein (CRP).ResultsExercise induced an overall hypercoagulable state characterized by significant decreases of TEG R and SP and an increase of α, increased concentrations of plasma vWf and decreased aPTT. In addition, a proinflammatory status was seen by a significant increase of serum CRP concentrations. Thyroid status was confirmed to be hypothyroid as training and exercise induced significant decrease of thyroxin (T4), free thyroxin (fT4) and thyroxin stimulating hormone (TSH) concentrations. Fibrinogen decreased significantly and PT increased. The training-induced changes showed correlation between T4, fT4 and aPTT and correlation between TSH and fibrinogen. Exercise-induced changes showed correlation between T4 and PT.ConclusionsExercise was associated with a hypercoagulable state and an increase of vWf concentration in this group of sled dogs. Decreased thyroid hormone concentrations after training and exercise were confirmed, but were associated with increased and not decreased vWf in this group of sled dogs.

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The Effect of Melatonin on Liver Superoxide Dismutase Activity, Serum Nitrate and Thyroid Hormone Levels.

Cited by 60 publications

References 26 publications

Melatonin protects porcine oocyte in vitro maturation from heat stress

Melatonin protects porcine oocyte in vitro maturation from heat stress

Preventive effect of melatonin on the progression of α‐naphthylisothiocyanate‐induced acute liver injury in rats

Exercise induced hypercoagulability, increased von Willebrand factor and decreased thyroid hormone concentrations in sled dogs

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