The Effect of Medium Modification and Selected Precursors on Sterol Production by Short-Term Callus Cultures of Euphorbia tirucalli

Biesboer, David D.; Mahlberg, Paul G.

doi:10.1021/np50006a011

Cited by 26 publications

(4 citation statements)

References 14 publications

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“…3). Latex, as from Euphorbiu, is physiologically active in the synthesis and accumulation of diverse secondary products including triterpenes (Biesboer & Mahlberg, 1979;Groeneveld, 1976;Nemethy, Skrukrud, Piazza & Calvin, 1983;Ponsinet & Ourisson, 1968). Similar activities leading to the formation of other secondary products such as alkaloids and glycosides are present in laticifers in other genera and species.…”

Section: B Hippomanoineae (Non-articulated Laticifers)mentioning

confidence: 99%

Laticifers and the classification of Euphorbia: the chemotaxonomy of Euphorbia esula L.

Mahlberg

Davis

Galitz

et al. 1987

Botanical Journal of the Linnean Society

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Laticifers and the classification of Euphorbia: the chemotaxonomy of Euphorbia esula L. Articulated and non‐articulated laticifer cells represent distinctive cell types of relatively recent origin and occur in only a few families. Both types are of separate phylogenetic origin, reflecting independent evolutionary trends in the Euphorbiaceae. Supra‐generic groupings of this family can be segregated into three taxonomic units using the laticifer character; with either articulated laticifers, non‐articulated laticifers, or no laticifers. Such units may reflect more natural assemblages than now represented in the classification of this family. Laticifers possess chemical and morphological features of potential application as taxonomic characters to aid in delimiting species and interpreting evolutionary trends. The triterpenoid profile from latex of Euphorbia species has been shown to be diagnostic for a taxon. The qualitative and quantitative composition show a high level of stability under diverse environmental and physiological conditions indicating a genetic basis for triterpenoid synthesis. Triterpenoid profiles of known accessions of European E. esula L. and related presumptive taxa from North America readily separated them into distinctive chemotaxa that include one for E. esula L., whereas morphological features were found inadequate for separating accessions to presumptive taxa. Identification of adventive spurges in North America requires diagnostic analyses of Eurasian leafy spurges for comparison. Laticifer characters used in conjunction with relevant morphological features will provide a broadened insight into phylogenetic relationships with the Euphorbiaceae.

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Section: B Hippomanoineae (Non-articulated Laticifers)mentioning

confidence: 99%

Laticifers and the classification of Euphorbia: the chemotaxonomy of Euphorbia esula L.

Mahlberg

Davis

Galitz

et al. 1987

Botanical Journal of the Linnean Society

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“…The sitosterol level in Solanum xanthocarpum decreased after indole acetic acid treatment (Heble et al, 197 1). By contrast, naphthalene acetic acid caused an increase in sterol synthesis in mung bean tissue (Geuns & Vendrig, 1974;Geuns, 1975), indole acetic acid increased sterol production in Euphorbia tirucalli callus cultures (Biesboer & Mahlberg, 1979) and removal of 2,4-dichlorophenoxyacetic acid from the growth medium of a carrot cell tissue culture resulted in a decrease in [2-"Clmevalonate incorporation into sterol (Nishi & Tsuritani, 1983).…”

Section: The Influence Of Plant Hormones On Sterol Synthesismentioning

confidence: 99%

How is sterol synthesis regulated in higher plants?

Goad

1983

Biochemical Society Transactions

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548BIOCHEMICAL SOCIETY TRANSACTIONS dione); 1 1flOH-P (1 1/?-hydroxyprogesterone; 1 1 /?-hydroxypregn-4ene-3,20-dione); 1 I-0x0-P (1 1-oxoprogesterone; pregn-4-ene-3,11,20trione); 21-d-F (2 I-deoxycortisol; 11/?,17a-dihydroxypregn-4-ene-3,20dione); 2 1 -d-E (2 1-deoxycortisone; 17a-hydroxypregn-4-ene-3,11,20trione); 1 1-deoxycortisol (Reichstein's substance S ; cortexolone; 1 7~2 l-dihydroxypregn-4-ene-3,20-dione); DHS (dihydro substance S; dihydro-1 1-deoxycortisol; 1 7a,2 1 -dihydroxy-5/?,pregnane-3,20-dione); THS (5fltetrahydro substance S ; tetrahydro-I 1-deoxycortisol; 3/?,17a,21-trihydroxy-5/?-pregnan-20-one); DOC (1 l-deoxycorticosterone; cortexone; 2 1 -hydroxypregn-4-ene-3,2O-dione); TH-DOC (tetrahydro-1 1-deoxycorticosterone; 342 l-dihydroxy-5/?-pregnan-20one); aldosterone (1 lp,2 l-dihydroxy-pregn-4-ene-3,18,2O-trione); 2a-Me,9a-FI-F (9a-fluoro-2a-methyl-1 I/?, 17a,21-trihydroxypregn-4ene-3,20-dione); glycyrrhetinic acid (glycyrrhetic acid; 3Fhydroxy-1 I-oxo-olean-12-en-30-oic acid); brassinolide [2 a,3 a,(ZZR)-(23R)-tetra hydroxy-(24S)-methyl-B-homo-7-oxa-5a-cholestan-6-onel.

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“…Studies have shown that the latex of Euphorbia cyparissias, E. helioscopia, and E. erythrea can synthesize triterpenes in vitro (PONSINET & OURISSON 1967, 1968. BIESBOER & MAHLBERG (1979) demonstrated the conversion of mevalonic acid into squalene and triterpenes by freshly tapped latex from E. tirucalli. Recently, NEMETHyet al (1983) reported the in vitro synthesis oftriterpenols and esterified triterpenes from labelled precursors of E. lathyris latex.…”

Section: Introductionmentioning

confidence: 96%

Sites of in-Vitro Triterpene Synthesis in Euphorbia Latex

Groeneveld

Furr

Mahlberg

1987

Acta Botanica Neerlandica

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SUMMARY Latices of Euphorbia species utilize labelled acetate and mevalonate for triterpene synthesis under in vitro conditions. Triterpene synthesis was detected at two sites in fractionated latex, viz. in particle‐containing vesicles and in a fraction consisting of amyloplasts, often associated with a tubular membrane system. Attempts to purify these fractions lead to a loss of triterpene synthesis in vitro. Latex particles were successfully purified by means of gel filtration procedures. Radioactive triterpenes were traced in these purified particles after incubation of the latex with (2–14C) acetate or (2–14C) mevalonate. The newly synthesized triterpenes were firmly attached to the particles of all occurring sizes. Although the fraction containing the amyloplasts was found to be very active in triterpene synthesis, no radioactive latex particles could be isolated from this fraction.

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The Effect of Medium Modification and Selected Precursors on Sterol Production by Short-Term Callus Cultures of Euphorbia tirucalli

Cited by 26 publications

References 14 publications

Laticifers and the classification of Euphorbia: the chemotaxonomy of Euphorbia esula L.

Laticifers and the classification of Euphorbia: the chemotaxonomy of Euphorbia esula L.

How is sterol synthesis regulated in higher plants?

Sites of in-Vitro Triterpene Synthesis in Euphorbia Latex

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