The effect of mechanical strain on soft (cardiovascular) and hard (bone) tissues

Boccafoschi, Francesca; Mosca, C.; Ramella, Martina; Valente, Guido; Cannas, M.

doi:10.4161/cam.23020

Cited by 12 publications

(12 citation statements)

References 81 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The lack of change in COL1A expression could to some extent be expected, given the very high level of expression of this gene (multifold over the housekeeping PRS18). These findings agree with the well‐accepted notion that the mechanical environment is important for maintaining the phenotype and functionality of terminally differentiated cells . Furthermore, the hypothesis that mechanical strain acts as a stimulator to induce differentiation of MSCs into osteoblasts has been experimentally supported by previous studies …”

Section: Discussionsupporting

confidence: 90%

See 1 more Smart Citation

Mechanoresponses of human primary osteoblasts grown on carbon nanotubes

Kroustalli

Kotsikoris

Karamitri

et al. 2014

J Biomedical Materials Res

View full text Add to dashboard Cite

Bone mechanotransduction is strongly influenced by the biomaterial properties. A good understanding of these mechanosensory mechanisms in bone has the potential to provide new strategies in the highly evolving field of bone tissue engineering. The aim of the present investigation was to study the interactive effects of local mechanical stimuli on multiwalled carbon nanotubes (MWCNTs)/osteoblast interface, using an in vitro model that allows the study of cell growth, attachment and differentiation. Strain was applied at physiological levels [strain magnitudes 500 microstrain (μɛ), at frequency of load application 0.5 Hz]. The effect of mechanical strain and substrate was thus studied by measuring the messenger RNA expression of alkaline phosphatase, vinculin, collagen 1A, and integrins β1, β3, α4, and αv, using real-time quantitative polymerase chain reaction. The osteoblasts grown on MWCNTs displayed quick adaptation to the new environment by modulating the expression of key adhesion integrins. Furthermore, the addition of mechanical strain interplayed with the extracellular matrix and was efficiently transduced by cells grown on MWCNTs, providing stronger adhesion and survival. MWCNTs are therefore a material perfectly compatible with osteoblast differentiation, adhesion, and growth, and should be further evaluated, to derive new-generation biomaterial scaffolds for the treatment of skeletal defects which require bone reconstruction.

show abstract

Section: Discussionsupporting

confidence: 90%

“…A large body of experimental evidence has highlighted the effects of physical forces such as tension, compression, gravity or shear stress at the cell level. Direct application of mechanical stresses to cultured cells can induce or modify cell differentiation, grown, and migration as well as gene expression …”

Section: Discussionmentioning

confidence: 99%

Mechanoresponses of human primary osteoblasts grown on carbon nanotubes

Kroustalli

Kotsikoris

Karamitri

et al. 2014

J Biomedical Materials Res

View full text Add to dashboard Cite

show abstract

“…Degeneration of bone with aging is a complex phenomenon, with a decrease in bone quality and quantity being affected by biochemical signaling in paracrine, autocrine and systemic manners. The complexity of bone remodeling is further highlighted by how changes in these facets of regulation occur in response to environmental stimuli such as diet and mechanical loading (Riddle et al, 2014; Boccafoschi et al, 2013). As the circadian clock regulates many aspects of physiology, dissecting exact mechanisms requires further investigation.…”

Section: 0 Discussionmentioning

confidence: 99%

Deficiency of circadian clock protein BMAL1 in mice results in a low bone mass phenotype

Samsa

Vasanji

Midura

et al. 2016

Bone

View full text Add to dashboard Cite

The circadian clock is an endogenous time keeping system that controls the physiology and behavior of many organisms. The transcription factor Brain and Muscle ARNT-like Protein 1 (BMAL1) is a component of the circadian clock and necessary for clock function. Bmal1−/− mice display accelerated aging and many accompanying age associated pathologies. Here, we report that mice deficient for BMAL1 have a low bone mass phenotype that is absent at birth and progressively worsens over their lifespan. Accelerated aging of these mice is associated with the formation of bony bridges occurring across the metaphysis to the epiphysis, resulting in shorter long bones. Using micro-computed tomography we show that Bmal1−/− mice have reductions in cortical and trabecular bone volume and other micro-structural parameters and a lower bone mineral density. Histology shows a deficiency of BMAL1 results in a reduced number of active osteoblasts and osteocytes in vivo. Isolation of bone marrow derived mesenchymal stem cells from Bmal1−/− mice demonstrate a reduced ability to differentiate into osteoblasts in vitro, which likely explains the observed reductions in osteoblasts and osteocytes, and may contribute to the observed osteopenia. Our data support the role of the circadian clock in the regulation of bone homeostasis and shows that BMAL1 deficiency results in a low bone mass phenotype.

show abstract

“…Mechanotransduction occurs through several different pathways including the opening of stretch activated ion channels, changes in integrin tension and signaling, the release of proteins from the extracellular matrix, the turnover of intracellular fibers such as actin, the exposure of “hidden” binding domains on cell surface proteins, and changes in spatial chemical gradients (14, 39). Several signaling pathways have been identified as being mechanosensative, including focal adhesion kinases (FAKs) and mitogen activated kinases (MAPKs) such as c-jun N terminal kinase (JNK) and extracellular related kinase (ERK) (39).…”

Section: Biomechanics Of Cardiac Function Post-mimentioning

confidence: 99%

Biomechanics of Cardiac Function

Voorhees

Han

2015

Comprehensive Physiology

View full text Add to dashboard Cite

The heart pumps blood to maintain circulation and ensure the delivery of oxygenated blood to all the organs of the body. Mechanics play a critical role in governing and regulating heart function under both normal and pathological conditions. Biological processes and mechanical stress are coupled together in regulating myocyte function and extracellular matrix structure thus controlling heart function. Here we offer a brief introduction to the biomechanics of left ventricular function and then summarize recent progress in the study of the effects of mechanical stress on ventricular wall remodeling and cardiac function as well as the effects of wall mechanical properties on cardiac function in normal and dysfunctional hearts. Various mechanical models to determine wall stress and cardiac function in normal and diseased hearts with both systolic and diastolic dysfunction are discussed. The results of these studies have enhanced our understanding of the biomechanical mechanism in the development and remodeling of normal and dysfunctional hearts. Biomechanics provide a tool to understand the mechanism of left ventricular remodeling in diastolic and systolic dysfunction and guidance in designing and developing new treatments.

show abstract

The effect of mechanical strain on soft (cardiovascular) and hard (bone) tissues

Cited by 12 publications

References 81 publications

Mechanoresponses of human primary osteoblasts grown on carbon nanotubes

Mechanoresponses of human primary osteoblasts grown on carbon nanotubes

Deficiency of circadian clock protein BMAL1 in mice results in a low bone mass phenotype

Biomechanics of Cardiac Function

Contact Info

Product

Resources

About