The Effect of Maturation Diets on the Reproductive Output of the White‐striped Cleaner Shrimp, Lysmata amboinensis

Abstract

Aquaculture production of the cleaner shrimp Lysmata amboinensis, a high‐valued marine ornamental, has been problematic because of prolonged larval development punctuated by periods of mortality. Broodstock maturation diets have been shown to affect fecundity and offspring quality and early survival. The common practice in shrimp culture is the use of fresh frozen foods supplemented with artificial diets. The objective of the study was to identify a suitable maturation diet (natural or artificial) for L. amboinensis. Six diets, comprised of squid, mussel, adult Artemia, a commercial feed, and combinations of the aforementioned, were fed to L. amboinensis for four reproductive cycles. Broodstock fed the squid–mussel diet lost a large proportion of the eggs during incubation, with decreased larval production (P < 0.05). In contrast, broodstock fed Artemia retained the highest proportion of the egg mass; however, hatchability was poor, resulting in low larval numbers per hatch. The Artemia and squid–mussel diets produced significantly fewer larvae than the combination or commercial diets (P < 0.05). In L. amboinensis, a maturation diet consisting of natural feeds alone resulted in poor reproductive performance and partial or complete replacement with an artificial diet was feasible.

Evaluation of lipid-enriched Artemia on the reproductive performance of the white-striped cleaner shrimp, Lysmata amboinensis

“…Growth in the 2-month study was also satisfactory, and although towards the lower end, still within the previously observed range for the species of 7-11% (Tziouveli et al 2011). There were, however, marked differences in the reproductive output among shrimp in different dietary treatment groups.…”

“…Embryo hatchability in the current study improved significantly when DHA-boosted Artemia were fed to L. amboinensis broodstock. In a previous comparison of maturation diets for L. amboinensis, a commercial pellet (Kuruma prawn diet, Ebi Star, Higashimaru, Japan) yielded high larval production (Tziouveli et al 2011), and when combining those results with the findings of this study, elevated DHA concentrations of 11-14% in broodstock diets intended for L. amboinensis are recommended. During the study, it was noted that shrimp egg production and attachment proceeded despite the low concentration of ARA and EPA in the diets, and in shrimp fed ''unenr'' Artemia of a DHA absence as well, and perhaps suggests a minimal role of these n-6 and n-3 HUFAs during this phase of reproduction.…”

“…Additionally, maternal grooming activities, stimulated by environmental stress factors or disease, have been related to egg mass losses in crustaceans (Kuris 1991;Oh and Hartnoll 1999). Egg loss has been noted in L. amboinensis regardless of the maturation diet, although diet has been shown to largely affect the extent of such loss (Tziouveli and Smith 2009;Tziouveli et al 2011). Therefore, it is not unusual for a portion of the spawned brood to be lost during embryo incubation.…”

“…In a previous study by the authors, feeding the cleaner shrimp, Lysmata amboinensis, solely on frozen adult Artemia biomass, on-grown on a mix of algae, resulted in a low number of larvae, compared with other fresh frozen and pelleted diets, and was attributed to poor embryo hatchability and the absence of DHA (Tziouveli et al 2011). The aims of this study were to further investigate the suitability of frozen adult Artemia biomass as a maturation diet for L. amboinensis, examine whether lipid enrichment improves the reproductive output of the cleaner shrimp and elucidate the role of EFAs in the reproductive performance of L. amboinensis.…”

Commercial squids: Characterization, assessment of potential health benefits/risks and discrimination based on mineral, lipid and vitamin E concentrations

“…No complete profile description was found for L. opalescens (Tziouveli et al, 2011) and no data was found for L. reynaudii. The slight differences ob-served with our study may be due to variations in geographical provenance, water temperature and salinity, diet season of catch, gender, sexual maturity (Ozogul et al, 2008) and analytical methodology.…”