The Effect of Matrix Extracellular Phosphoglycoprotein and Its Downstream Osteogenesis-related Gene Expression on the Proliferation and Differentiation of Human Dental Pulp Cells

Wei, Xi; Liu, Lu; Zhou, Xiaoyan; Zhang, Fang; Ling, Junqi

doi:10.1016/j.joen.2011.10.015

Cited by 17 publications

(8 citation statements)

References 32 publications

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“…We have shown that mouse molars with ablated Mepe exhibited reduced levels of other SIBLING transcripts as well as decreased levels of Enam , suggesting dysregulation of critical dentin and enamel matrix proteins that are essential for normal mineralization. These findings support previous in vitro studies where the overexpression of MEPE in dental pulp cells caused increased levels of odontoblast markers BSP, DSP (DSPP), and collagen type I and Enam (Wei et al 2012). Interestingly, Enam is a member of the SCPPs, which are rich in Pro and Gln (P/Q) and associated with enamel mineralization, located on 4q21 adjacent to the SIBLING gene cluster (Kawasaki 2011).…”

Section: Discussionsupporting

confidence: 91%

MEPE Localization in the Craniofacial Complex and Function in Tooth Dentin Formation

Gullard

Gluhak-Heinrich

Papagerakis

et al. 2016

J Histochem Cytochem.

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Matrix extracellular phosphoglycoprotein (MEPE) is an extracellular matrix protein found in dental and skeletal tissues. Although information regarding the role of MEPE in bone and disorders of phosphate metabolism is emerging, the role of MEPE in dental tissues remains unclear. We performed RNA in situ hybridization and immunohistochemistry analyses to delineate the expression pattern of MEPE during embryonic and postnatal development in craniofacial mineralizing tissues. Mepe RNA expression was seen within teeth from cap through root formation in association with odontoblasts and cellular cementoblasts. More intense expression was seen in the alveolar bone within the osteoblasts and osteocytes. MEPE immunohistochemistry showed biphasic dentin staining in incisors and more intense staining in alveolar bone matrix and in forming cartilage. Analysis of Mepe null mouse molars showed overall mineralized tooth volume and density of enamel and dentin comparable with that of wild-type samples. However, Mepe(-/-) molars exhibited increased thickness of predentin, dentin, and enamel over controls and decreased gene expression of Enam, Bsp, Dmp1, Dspp, and Opnby RT-PCR. In vitro Mepe overexpression in odontoblasts led to significant reductions in Dspp reporter activity. These data suggest MEPE may be instrumental in craniofacial and dental matrix maturation, potentially functioning in the maintenance of non-mineralized matrix.

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Section: Discussionsupporting

confidence: 91%

MEPE Localization in the Craniofacial Complex and Function in Tooth Dentin Formation

Gullard

Gluhak-Heinrich

Papagerakis

et al. 2016

J Histochem Cytochem.

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“…Whereas CST6 expression was downregulated in Finnsheep and the F1 crosses compared with Texel, the flushing diet appeared to minimize the expression of this gene in Texel, because it was downregulated in the Texel group receiving the flushing diet (within breed diet comparisons). To date, studies examining the functional aspects of MEPE have focused on humans and have highlighted a role in bone proliferation and differentiation [ 31 – 33 ]. Both CST6 and MEPE were downregulated in Finnsheep and upregulated in Texel.…”

Section: Resultsmentioning

confidence: 99%

Integrated ovarian mRNA and miRNA transcriptome profiling characterizes the genetic basis of prolificacy traits in sheep (Ovis aries)

et al. 2018

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BackgroundThe highly prolific breeds of domestic sheep (Ovis aries) are globally valuable genetic resources for sheep industry. Genetic, nutritional and other environmental factors affect prolificacy traits in sheep. To improve our knowledge of the sheep prolificacy traits, we conducted mRNA-miRNA integrated profiling of ovarian tissues from two pure breeds with large (Finnsheep) vs. small (Texel) litter sizes and their F1 crosses, half of which were fed a flushing diet.ResultsAmong the samples, 16,402 genes (60.6% known ovine genes) were expressed, 79 novel miRNAs were found, and a cluster of miRNAs on chromosome 18 was detected. The majority of the differentially expressed genes between breeds were upregulated in the Texel with low prolificacy, owing to the flushing diet effect, whereas a similar pattern was not detected in the Finnsheep. F1 ewes responded similarly to Finnsheep rather than displaying a performance intermediate between the two pure breeds.ConclusionsThe identification and characterization of differentially expressed genes and miRNAs in the ovaries of sheep provided insights into genetic and environmental factors affecting prolificacy traits. The three genes (CST6, MEPE and HBB) that were differentially expressed between the group of Finnsheep and Texel ewes kept in normal diet appeared to be candidate genes of prolificacy traits and will require further validation.Electronic supplementary materialThe online version of this article (10.1186/s12864-017-4400-4) contains supplementary material, which is available to authorized users.

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“…Another study examined the protein MEPE. Obviously, the protein plays an important role in the proliferation and osteogenic differentiation of dental pulp cells [37].…”

Section: Discussionmentioning

confidence: 99%

Complementing the pulp proteome via sampling with a picosecond infrared laser (PIRL)

et al. 2021

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Objectives The aim of this investigation was the detailed analysis of the human pulp proteome using the new picosecond infrared laser (PIRL)-based sampling technique, which is based on a completely different mechanism compared to mechanical sampling. Proteome analysis of healthy pulp can provide data to define changes in the proteome associated with dental disease. Material and methods Immediately after extraction of the entire, undamaged tooth, 15 wisdom teeth were deep frozen in liquid nitrogen and preserved at −80°C. Teeth were crushed, and the excised frozen pulps were conditioned for further analysis. The pulps were sampled using PIRL, and the aspirates digested with trypsin and analyzed with mass spectrometry. Pulp proteins were categorized according to their gene ontology terminus. Proteins identified exclusively in this study were searched in the Human Protein Atlas (HPA) for gaining information about the main known localization and function. Results A total of 1348 proteins were identified in this study. The comparison with prior studies showed a match of 72%. Twenty-eight percent of the proteins were identified exclusively in this study. Considering HPA, almost half of these proteins were assigned to tissues that could be pulp specific. Conclusion PIRL is releasing proteins from the dental pulp which are not dissolved by conventional sampling techniques. Clinical Relevance The presented data extend current knowledge on dental pulp proteomics in healthy teeth and can serve as a reference for studies on pulp proteomics in dental disease.

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The Effect of Matrix Extracellular Phosphoglycoprotein and Its Downstream Osteogenesis-related Gene Expression on the Proliferation and Differentiation of Human Dental Pulp Cells

Cited by 17 publications

References 32 publications

MEPE Localization in the Craniofacial Complex and Function in Tooth Dentin Formation

MEPE Localization in the Craniofacial Complex and Function in Tooth Dentin Formation

Integrated ovarian mRNA and miRNA transcriptome profiling characterizes the genetic basis of prolificacy traits in sheep (Ovis aries)

Complementing the pulp proteome via sampling with a picosecond infrared laser (PIRL)

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