The effect of marcaine on muscle and non‐muscle cells in vitro

Schultz, Edward; Lipton, Bruce H.

doi:10.1002/ar.1091910308

Cited by 49 publications

(23 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…that undifferentiated myotubes are not susceptible to toxin assault, whereas fibres which have differentiated are susceptible, an observation similar to that made by Schultz & Lipton (1978) using Marcaine.…”

Section: The Response Ofdenervated Muscle To Taipoxinsupporting

confidence: 76%

MYOTOXIC ACTIVITY OF THE CRUDE VENOM AND THE PRINCIPAL NEUROTOXIN, TAIPOXIN, OF THE AUSTRALIAN TAIPAN, Oxyuranus scutellatus

Harris

Maltin

1982

British J Pharmacology

View full text Add to dashboard Cite

The crude venom of the Australian taipan, Oxyuranus scutellatus and its principal neurotoxin, taipoxin, were injected into the anterolateral aspect of one hind limb of the rat. The effects of the venom and toxin on the morphology and physiology on the underlying soleus muscles were examined. Both the crude venom and the toxin caused necrosis and degeneration of the muscle. Damage to the peripheral muscle fibres could be seen at the light microscopic level as early as 3 h after injection of the toxic compounds. The necrotic response was accompanied by an infiltration of phagocytic cells and an extensive oedema. The wet weight of the damaged muscles was almost doubled by 6 h. In individual muscle fibres, necrosis was associated with the disruption of the plasma membrane and the disorganization of the myofibrils. The basal lamina of the muscle fibres was left intact. Denervated mammalian muscles and innervated avian muscles were also destroyed by taipoxin, but immature avian muscle growing in tissue culture was resistant. Of the 3 subunits of taipoxin, only the basic α‐taipoxin was itself myotoxic. However, its potency was enhanced by the presence of the acid γ‐subunit. The role of the neutral β‐subunit is unclear. The period of necrosis and degeneration lasted for approximately 48 h, after which the muscle fibres began to regenerate. Regeneration took place within the surviving basal lamina, with the formation of myotubes by three days, and small, immature muscle fibres by five days. Regeneration was virtually complete by 21 days.

show abstract

Section: The Response Ofdenervated Muscle To Taipoxinsupporting

confidence: 76%

MYOTOXIC ACTIVITY OF THE CRUDE VENOM AND THE PRINCIPAL NEUROTOXIN, TAIPOXIN, OF THE AUSTRALIAN TAIPAN, Oxyuranus scutellatus

Harris

Maltin

1982

British J Pharmacology

View full text Add to dashboard Cite

show abstract

“…At this point interventional pain therapy, such as epidural analgesia can be introduced with much greater efficacy [12]. Tissue toxicity to LA has been documented in animal studies particularly at higher concentrations [2,3,4,6,7,8,11,13,14,15,16,17,18,19,20,25,29,31,32,33,34,37,38] but seldom observed using the recommended clinical concentrations in humans [1,15,22,23,24,26,28,31].…”

Section: Abstract · Zusammenfassung Discussionmentioning

confidence: 99%

Bupivacaine crystal deposits after long-term epidural infusion

et al. 2013

View full text Add to dashboard Cite

“…Dies mag sich v. a. dadurch erklären, dass skelettmuskuläre Schädigungen nach der Gabe von Lokalanästhetika zwar obligatorisch zu entstehen scheinen, in vielen Fällen jedoch funktionell inapparent bleiben und sich höchstwahrscheinlich innerhalb weniger Wochen zurückbil-den [14,28,29]. Andererseits kommen verschiedene Studien und Fallberichte zu dem Schluss, dass bestimmte lokoregionäre Verfahren mit einer bemerkenswert hohen post operativen Inzidenz an klinisch relevanten Dysfunktionen der Skelettmuskulatur einhergehen, die wiederum eindeutig auf direkte myotoxische Effekte von Lokalanästhetika zurückzu-führen sind [20,22,38,45,50]. In diesem Zusammenhang sind in erster Linie Retro-bzw.…”

Section: Myotoxizität Von Lokalanästhetikaunclassified

Myotoxizität von Lokalanästhetika

et al. 2007

View full text Add to dashboard Cite

Intramuscular injections of local anaesthetic agents regularly result in reversible muscle damage, with a dose-dependent extent of the lesions. All local anaesthetic agents that have been examined are myotoxic, whereby procaine produces the least and bupivacaine the most severe muscle injury. The histological pattern and the time course of skeletal muscle injury appear relatively uniform: hypercontracted myofibrils become evident directly after injection, followed by lytic degeneration of striated muscle sarcoplasmic reticulum myocyte edema and necrosis. Intriguingly, in most cases myoblasts, basal laminae and connective tissue elements remain intact which subsequently ensures complete muscular regeneration. Subcellular pathomechanisms of local anaesthetic myotoxicity are still not understood in detail. Increased intracellular Ca(2+) levels are suggested to be the most important element in myocyte injury, since denervation, inhibition of sarcolemmal Na(+) channels and direct toxic effects on myofibrils have been excluded as sites of action. Although experimental myotoxic effects are impressively intense and reproducible, only few case reports of myotoxic complications in patients after local anaesthetic administration have been published. In particular, the occurrence of clinically relevant myopathy and myonecrosis has been described after continuous peripheral blockades, infiltration of wound margins, trigger point injections, peribulbar and retrobulbar blocks.

show abstract

The effect of marcaine on muscle and non‐muscle cells in vitro

Cited by 49 publications

References 22 publications

MYOTOXIC ACTIVITY OF THE CRUDE VENOM AND THE PRINCIPAL NEUROTOXIN, TAIPOXIN, OF THE AUSTRALIAN TAIPAN, Oxyuranus scutellatus

MYOTOXIC ACTIVITY OF THE CRUDE VENOM AND THE PRINCIPAL NEUROTOXIN, TAIPOXIN, OF THE AUSTRALIAN TAIPAN, Oxyuranus scutellatus

Bupivacaine crystal deposits after long-term epidural infusion

Myotoxizität von Lokalanästhetika

Contact Info

Product

Resources

About