The Effect of Mammalian Sex Hormones on Polymorphism and Genomic Instability in the Common Bean (Phaseolus vulgaris L.)

Türkoğlu, Aras; Haliloğlu, Kamil; Balpınar, Özge; Öztürk, Halil İbrahim; Özkan, Güller; Poczai, Péter

doi:10.3390/plants11152071

Cited by 7 publications

(11 citation statements)

References 51 publications

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“…The concentration and quality of genomic DNA were measured with a Nanodrop spectrophotometer (Qiagen Qiaxpert, Qiagen, Hilden, Germany) (Thermo Fisher Scientific, Waltham, MA, USA) and run on 1.5% (w/v) agarose gel. Twenty primers were tested for iPBS-PCR amplification [65]. For iPBS analysis, a PCR reaction was carried out by Hosseinpour et al [22].…”

Section: Methodsmentioning

confidence: 99%

Effects of Zinc, Copper and Iron Oxide Nanoparticles on Induced DNA Methylation, Genomic Instability and LTR Retrotransposon Polymorphism in Wheat (Triticum aestivum L.)

Haliloğlu

Türkoğlu

Balpınar

et al. 2022

Plants

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Nanomaterials with unique and diverse physico-chemical properties are used in plant science since they improve plant growth and development and offer protection against biotic and abiotic stressors. Previous studies have explored the effects of such nanomaterials on different plant mechanisms, but information about the effects of nanomaterials on induced DNA methylation, genomic instability and LTR retrotransposon polymorphism in wheat is lacking. Therefore, the present study highlights the key role of nanoparticles in DNA methylation and polymorphism in wheat by investigating the effects of ZnO, CuO and γ-Fe3O4 nanoparticles (NPs) on mature embryo cultures of wheat (Triticum aestivum L.). Nanoparticles were supplemented with Murashige and Skoog (MS) basal medium at normal (1X), double (2X) and triple (3X) concentrations. The findings revealed different responses to the polymorphism rate depending on the nanoparticle type and concentration. Genomic template stability (GTS) values were used to compare the changes encountered in iPBS profiles. ZnO, CuO and γ-Fe3O4 NPs increased the polymorphism rate and cytosine methylation compared to the positive control while reducing GTS values. Moreover, non-γ-Fe3O4 NPs treatments and 2X ZnO and CuO NP treatments yielded higher polymorphism percentages in both MspI- and HpaII-digested CRED-iPBS assays and were thus classified as hypermethylation when the average polymorphism percentage for MspI digestion was considered. On the other hand, the 3X concentrations of all nanoparticles decreased HpaII and MspI polymorphism percentages and were thus classified as hypomethylation. The findings revealed that MS medium supplemented with nanoparticles had epigenetic and genotoxic effects.

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Section: Methodsmentioning

confidence: 99%

Effects of Zinc, Copper and Iron Oxide Nanoparticles on Induced DNA Methylation, Genomic Instability and LTR Retrotransposon Polymorphism in Wheat (Triticum aestivum L.)

Haliloğlu

Türkoğlu

Balpınar

et al. 2022

Plants

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“…Their physiological activity, genomic DNA instability, and epigenetic effects on plants have not yet been completely described, despite the fact that the amounts, receptors, and individual binding sites of MSHs in plants have been partially revealed [12]. Mammalian sex hormones can induce molecular changes by affecting genome stability and epigenetic mechanisms [13].…”

Section: Introductionmentioning

confidence: 99%

“…It has been shown that plants are able to maintain their survival cycle by exploiting changes in response to conditions in their environment [17]. In particular, there is evidence of substantial connections between hormones produced by plants and epigenetic signals produced by the plants [13,18]. The study of epigenetic changes can be approached from many different angles.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the technique is simple and cost-efficient [20,21]. Restriction enzyme-coupled digestion and inter-primer binding site (iPBS) methodology, also known as CRED-iPBS, is a method that is both efficient and cost-effective for studying the methylation status of plant DNA [13,[22][23][24]. iPBS retrotransposons constitute a marker system that relies on the presence of tRNA as a binding site for reverse transcriptase primers.…”

Section: Introductionmentioning

confidence: 99%

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Mammalian Sex Hormones as Steroid-Structured Compounds in Wheat Seedling: Template of the Cytosine Methylation Alteration and Retrotransposon Polymorphisms with iPBS and CRED-iBPS Techniques

Demirel,

Türkoğlu,

Haliloğlu

et al. 2023

Applied Sciences

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Phytohormones are chemical compounds found naturally in plants that have a significant effect on their growth and development. The increase in research on the occurrence of mammalian sex hormones (MSHs) in plants has prompted the need to investigate the functions performed by these hormones in plant biology. In the present study, we investigated the effects of MSHs on DNA damage and DNA methylation of wheat (Triticum aestivum L.) during the seedling growth stage, using the CRED-iPBS (coupled restriction enzyme digestion/inter primer binding site) assay and iPBS analysis to determine DNA methylation status. Exogenous treatment with four MSHs (17-β-estradiol, estrogen, progesterone, and testosterone) was carried out at four different concentrations (0, 0.05, 0.5, and 5 µM). The highest genomic template stability (GTS) value (80%) was observed for 5 µM 17-β-estradiol, 0.5 µM testosterone, and 0.05 µM estrogen, while the lowest value (70.7%) was observed for 5 µM progesterone and 0.5 µM estrogen. The results of the CRED-iPBS analysis conducted on MspI indicate that the 0.05 µM estrogen-treated group had the highest polymorphism value of 40%, while the 5 µM progesterone-treated group had the lowest value of 20%. For HpaII, treatment with 0.5 µM 17-β-estradiol had the highest polymorphism value of 33.3%, while the group treated with 0.05 µM 17-β-estradiol and 0.05 µM progesterone had the lowest value of 19.4%. In conclusion, MSH treatments altered the stability of the genomic template of wheat plants and affected the cytosine methylation status at the seedling growth stage. Upon comprehensive examination of the results, it was seen that the employed methodology successfully detected alterations in cytosine methylation of genomic DNA (gDNA), as well as changes in the pattern of genomic instability.

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“…Addition of sex hormones greatly increased the proline and antioxidant enzyme activity in vitro cultured triticale mature embryos (Uysal and Bezirganoğlu, 2017). Responses of in vitro cultured wheat and bean plants to different types and concentrations of sex hormones varied signi cantly and differences were attributed to epigenetic modi cations (Türkoğlu 2022a(Türkoğlu , 2022b. Latter reports revealed that there is signi cant interaction between sex hormone type by concentration.…”

Section: Introductionmentioning

confidence: 99%

Effect of Mammalian Sex Hormones on Wheat Mature Embryo Culture

Otaran

Çağlar

Haliloğlu

et al. 2022

Preprint

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Mammalian sex hormones (MSHs) are synthesized by various plant species and known play critical roles in growth, development, and reproduction. External and endogenous use of MSHs resulted in different response in different plant species. However, effects of different MSHs with concentrations on different wheat genetic backgrounds have not been studied in vitro. Therefore, three wheat cultivars (cvs. Palandöken 97, Nenehatun, Kırik) and four MSHs (17 β-estradiol, estrogen, progesterone, and testosterone) with three concentrations of 10-4, 10-5, 10-6 mM were used to investigate callus formation and embryonic callus formation rates as well as number of regenerated plants. Our results showed that wheat genotypes were significantly influenced by the type of MSH and its concentration. In addition, effect of hormones may vary with respect to growth or development of cells or plants. The highest callus formation rate was obtained on cv. Palandöken 97 with 10-4 mM testosterone hormone. Embryogenic callus formation rate with respect to number of explants was the highest on Palandöken 97 in 17β-estradiol with 10 -4 mM while embryogenic callus formation rate with respect to number of calli was the highest on cv. Kırik in progesterone and 10-4 mM concentration. On the other hand, the highest regenerated plants were observed on cv. Palandöken 97 in 10-6 mM 17β-estradiol and 10-6 Mm progesterone. Such contrasting results may indicate the potential utilization of suitable wheat genotypes, MSHs and doses in wheat tissue culture which is a prerequisite in wheat transformation experiments.

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The Effect of Mammalian Sex Hormones on Polymorphism and Genomic Instability in the Common Bean (Phaseolus vulgaris L.)

Cited by 7 publications

References 51 publications

Effects of Zinc, Copper and Iron Oxide Nanoparticles on Induced DNA Methylation, Genomic Instability and LTR Retrotransposon Polymorphism in Wheat (Triticum aestivum L.)

Effects of Zinc, Copper and Iron Oxide Nanoparticles on Induced DNA Methylation, Genomic Instability and LTR Retrotransposon Polymorphism in Wheat (Triticum aestivum L.)

Mammalian Sex Hormones as Steroid-Structured Compounds in Wheat Seedling: Template of the Cytosine Methylation Alteration and Retrotransposon Polymorphisms with iPBS and CRED-iBPS Techniques

Effect of Mammalian Sex Hormones on Wheat Mature Embryo Culture

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