The effect of low-level helium-neon (HeNe) laser radiation on the secretion of cytokines that promote chronic graft rejection – An in vitro study

Mehrsai, Abdolrasoul; Afsharpad, Mandana; Mohydin, Mandana; Ansari, Bita; Pourmand, Gholamreza; Nikbin, Behrooz

doi:10.1016/j.mla.2009.03.001

Cited by 5 publications

(5 citation statements)

References 24 publications

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“…However, only five studies published up to 2014 evaluated the effect of red light on macrophages or monocytes [21–23,25,33]. Three of these studies employed laser [22,23,33] and two employed a light emitting diode (LED) [21,25]. The power output ranged from 2.3 mW to 2.5 W and the fluence ranged from 0.01 to 10,714 J/cm 2 .…”

Section: Discussionmentioning

confidence: 99%

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Photobiomodulation with 660-nm and 780-nm laser on activated J774 macrophage-like cells: Effect on M1 inflammatory markers

Fernandes

Souza

Mesquita‐Ferrari

et al. 2015

Journal of Photochemistry and Photobiology B: Biology

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M1 profile macrophages exert a major influence on initial tissue repair process. Few days after the occurrence of injury, macrophages in the injured region exhibit a M2 profile, attenuate the effects of the M1 population, and stimulate the reconstruction of the damaged tissue. The different effects of macrophages in the healing process suggest that these cells could be the target of therapeutic interventions. Photobiomodulation has been used to accelerate tissue repair, but little is known regarding its effect on macrophages. In the present study, J774 macrophages were activated to simulate the M1 profile and irradiated with two different sets of laser parameters (780 nm, 70 mW, 2.6 J/cm2, 1.5 s and 660 nm, 15 mW, 7.5 J/cm2, 20 s). IL-6, TNF-α, iNOS and COX-2 gene and protein expression were analyzed by RT-qPCR and ELISA. Both lasers were able to reduce TNF-α and iNOS expression, and TNF-α and COX-2 production, although the parameters used for 780 nm laser provided an additional decrease. 660 nm laser parameters resulted in an up-regulation of IL-6 expression and production. These findings imply a distinct, time-dependent modulation by the two different sets of laser parameters, suggesting that the best modulation may involve more than one combination of parameters.

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Section: Discussionmentioning

confidence: 99%

“…J774 macrophages were incubated with 0.2 µg/mL interferon-γ (IFN-γ, Sigma, St. Louis, MO, USA) and 1 µg/mL lipopolysaccharide (LPS; serotype O26:B6 from Escherichia coli ) (Sigma) in DMEM 5% (FBS) for 24 h [24,26,33]. …”

Section: Methodsmentioning

confidence: 99%

Photobiomodulation with 660-nm and 780-nm laser on activated J774 macrophage-like cells: Effect on M1 inflammatory markers

Fernandes

Souza

Mesquita‐Ferrari

et al. 2015

Journal of Photochemistry and Photobiology B: Biology

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“…Previous studies that assessed the effects of LLLT and LED ( light emitting diode ) therapy on macrophages or their precursors (monocytes) did not evaluate their mitochondrial activity but did evaluate various functions of such cells 13 , 14 , 18 - 20 , 29 - 31 .…”

Section: Discussionmentioning

confidence: 99%

Effect of low-level laser therapy on the modulation of the mitochondrial activity of macrophages

Souza¹,

Mesquita‐Ferrari²,

Silva

et al. 2014

Braz. J. Phys. Ther.

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BACKGROUND: Macrophages play a major role among the inflammatory cells that invade muscle tissue following an injury. Low-level laser therapy (LLLT) has long been used in clinical practice to accelerate the muscle repair process. However, little is known regarding its effect on macrophages. OBJECTIVE: This study evaluated the effect of LLLT on the mitochondrial activity (MA) of macrophages. METHOD: J774 macrophages were treated with lipopolysaccharide (LPS) and interferon - gamma (IFN-γ) (activation) for 24 h to simulate an inflammatory process, then irradiated with LLLT using two sets of parameters (780 nm; 70 mW; 3 J/cm2 and 660 nm; 15 mW; 7.5 J/cm2). Non-activated/non-irradiated cells composed the control group. MA was evaluated by the cell mitochondrial activity (MTT) assay (after 1, 3 and 5 days) in three independent experiments. The data were analyzed statistically. RESULTS: After 1 day of culture, activated and 780 nm irradiated macrophages showed lower MA than activated macrophages, but activated and 660 nm irradiated macrophages showed MA similar to activated cells. After 3 days, activated and irradiated (660 nm and 780 nm) macrophages showed greater MA than activated macrophages, and after 5 days, the activated and irradiated (660 nm and 780 nm) macrophages showed similar MA to the activated macrophages. CONCLUSIONS: These results show that 660 nm and 780 nm LLLT can modulate the cellular activation status of macrophages in inflammation, highlighting the importance of this resource and of the correct determination of its parameters in the repair process of skeletal muscle.

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“…Numerous studies in the literature have reported the therapeutic effects of red and infrared light sources for the treatment of tissue injuries, [ 4, 10, 11, 14, 16, 51 ] the modulation of the inflammatory response of monocytes/macrophages [ 4, 8–10, 12 ] and systemic applications of PBM. [ 47, 52, 53 ] However, other studies have suggested that blue, green and yellow/amber light sources could be effective for both local and systemic PBM, [ 54–60 ] while others have reported the effects of a combination of red and infrared wavelengths.…”

Section: Discussionmentioning

confidence: 99%

“…Photobiomodulation (PBM) is capable of affecting both the degree of activation and the phenotype of leukocytes in tissue in the different phases of the repair process. [ 8–13 ] This therapeutic modality employs light sources (lasers and light‐emitting diodes) for the noninvasive, nonthermal treatment of injured tissue, among which red (600‐700 nm) and near infrared (770‐1200 nm) wavelengths are the most commonly employed. [ 14–16 ]…”

Section: Introductionmentioning

confidence: 99%

Effects of the phenotypic polarization state of human leukocytes on the optical absorbance spectrum

Sousa

Silva

Rodrigues

et al. 2021

Journal of Biophotonics

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This study evaluated the optical absorbance spectrum of human monocytes, neutrophils and lymphocytes polarized, or not, to the inflammatory or immunoregulatory phenotypes. Peripheral human blood leukocytes were isolated and polarized (10 ng/mL) with LPS or IL‐4 + LPS for 2 hours. After polarization, cells were washed and incubated for an additional 24 hours (monocytes and lymphocytes) or 12 hours (neutrophils). Next, cells were collected to evaluate the optical absorbance spectrum. The three types of leukocytes exhibited absorbance in the region from 450 to 900 nm, with greater absorbance at wavelengths lower than 570 nm. Lymphocytes had a second region of greater absorbance between 770 and 900 nm. Inflammatory monocytes and lymphocytes showed increased absorbance of blue, green and yellow wavelengths (monocytes), as well as red and infrared wavelengths (monocytes and lymphocytes). Immunoregulatory polarization altered the absorbance of monocytes and lymphocytes very little. Neutrophils treated with LPS or LPS + IL‐4 exhibited lower absorbance at wavelengths higher than 575 nm compared to untreated cells. The present findings showed that leukocytes exhibit greater absorbance in regions of the spectrum that have not been much used in photobiomodulation (PBM), and the polarization of these cells can affect their capacity to absorb light. Taken together, these results suggest new perspectives in the use of PBM in the clinical setting depending on the wavelengths and the stage of the inflammatory process.

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The effect of low-level helium-neon (HeNe) laser radiation on the secretion of cytokines that promote chronic graft rejection – An in vitro study

Cited by 5 publications

References 24 publications

Photobiomodulation with 660-nm and 780-nm laser on activated J774 macrophage-like cells: Effect on M1 inflammatory markers

Photobiomodulation with 660-nm and 780-nm laser on activated J774 macrophage-like cells: Effect on M1 inflammatory markers

Effect of low-level laser therapy on the modulation of the mitochondrial activity of macrophages

Effects of the phenotypic polarization state of human leukocytes on the optical absorbance spectrum

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