The effect of low level laser irradiation on adult human adipose derived stem cells

Abstract

This study investigated the effect of low level laser irradiation on primary cultures of adult human adipose derived stem cells (ADSC) using a 635-nm diode laser, at 5 J/cm(2) with a power output of 50.2 mW and a power density of 5.5 mW/cm(2). Cellular morphology did not appear to change after irradiation. Using the trypan blue exclusion test, the cellular viability of irradiated cells increased by 1% at 24 h and 1.6% at 48 h but was not statistically significant. However, the increase of cellular viability as measured by ATP luminescence was statistically significant at 48 h (p < 0.05). Proliferation of irradiated cells, measured by optical density, resulted in statistically significant increases in values compared to nonirradiated cells (p < 0.05) at both time points. Western blot analysis and immunocytochemical labeling indicated an increase in the expression of stem cell marker beta1-integrin after irradiation. These results indicate that 5 J/cm(2) of laser irradiation can positively affect human adipose stem cells by increasing cellular viability, proliferation, and expression of beta1-integrin.

Effects of low‐level laser therapy on proliferation and differentiation of murine bone marrow cells into osteoblasts and osteoclasts

“…Although LLLT can increase cell proliferation if performed at early culture time, Ozawa et al [10] have shown that irradiation after 6 days of culture had no effect on cellular proliferation. In two reports, LLLT protocol was found to increase MSCs proliferation, but in both cases, cells were irradiated after one passage, which is known to reduce their innate proliferation rate [11,12]. Moreover Shimizu et al [13] reported that low-level laser effect on proliferation was mediated by the secretion of IGF-1 on rat calvaria cells.…”

The influence of continuous low-intensity laser radiation at the red (635 nm) and green (525 nm) wavelengths on the human mesenchymal stem cells in vitro: a review of the literature and original investigations

“…Несмотря на значительный разброс энергетиче-ских параметров лазерного воздействия в исследо-ваниях по изучению влияния НИЛИ на пролифера-цию МСК, данные литературы в отношении опти-мальной ЭП для непрерывного НИЛИ с длиной волны 633-635 нм находятся в достаточно узком диапазоне -0,3-0,5 Дж/см 2 , при этом ПМ варьи-рует в значительно более широких пределах, от 3-6,61 мВт/см 2 [18][19][20][21][22][23] a -фотография клеток в проходящем свете. Ув.…”

The role of adipose derived stem cells, smooth muscle cells and low intensity laser irradiation (LILI) in tissue engineering and regenerative medicine

“…They have also been differentiated into smooth muscle cells that are functional [27]. These cells have also shown increased viability and proliferation when exposed to LILI alone [5] and, as well as, in combination with epidermal growth factor (EGF) [28]. Recent studies have demonstrated the functional neural differentiation of human ADSCs [29].…”

“…Results have shown that LILI can produce negative and positive effects on stem cells. Positive results include inhibition of inflammation and increased cell viability and proliferation [4,5]. Due to ease of isolation and plasticity [6], ADSCs hold great promise for tissue engineering and regenerative medicine [7].…”