The effect of long zona dissection using ICSI pipettes for mechanical assisted hatching in vitrified-thawed blastocyst transfers

Sun, Sheng-Ta; Choi, Jong-Ryeol; Son, Jung-Bin; Joo, Jong‐Kil; Ko, Gyoung Rae; Lee, Kyusup

doi:10.1007/s10815-012-9872-1

Cited by 14 publications

(12 citation statements)

References 13 publications

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“…Due to this result, we think the success of conception after FET is primarily affected by embryo quality, such as condition of inner cell mass, trophectoderm, and hatching process. In our previous study, the hatching process is important for implantation in FET cycles because zona hardening commonly occurs more than fresh ET cycles and we reported implantation and clinical pregnancy rates of 40.9% and 63.0% by GnRH antagonist protocol with FET assisted by long zona dissection technique …”

Section: Discussionmentioning

confidence: 88%

Measurement of endometrial and uterine vascularity by transvaginal ultrasonography in predicting pregnancy outcome during frozen–thawed embryo transfer cycles

Son

Jeong

Joo

et al. 2014

J of Obstet and Gynaecol

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Aim: An appropriate endometrial condition and vascular supply are usually considered essential for implantation of an embryo. This study was performed to assess the role of endometrial and uterine vascularity status measurement in predicting pregnancy outcome during frozen-thawed embryo transfer cycles. Material and Methods: A total of 70 infertile women were recruited with controlled ovarian stimulation followed by oocyte retrieval. After in vitro fertilization or intracytoplasmic sperm injection, embryos were cultured to blastocysts and blastocysts with good quality were selected for cryopreservation. After endometrial preparation, vitrified blastocysts were thawed and assisted hatching by zona dissection was performed. On the day of embryo transfer, endometrial thickness (EMT), resistance index (RI) and pulsatility index (PI) of sub-endometrial artery (SEA) and uterine artery (UA) were obtained by transvaginal sonography. The women were divided into the pregnant and non-pregnant groups, and these variables were compared between the two groups. Results: Patients' general demographic characteristics were not statistically different between the pregnant and non-pregnant groups. The overall implantation rate, clinical pregnancy rate, and ongoing pregnancy rate were 31.1%, 41.4% and 28.6%, respectively. Twenty-nine patients who conceived had average EMT, RI of SEA, PI of SEA, RI of UA, and PI of UA values of 9.15 mm, 0.91, 2.42, 0.95, and 3.37, respectively. Forty-one patients who did not conceive had average EMT, RI of SEA, PI of SEA, RI of UA, and PI of UA values of 9.31 mm, 1.01, 2.56, 0.94, and 3.00, respectively. In the two groups, none of the variables was statistically different (P > 0.05). Conclusions: EMT and blood flow index of endometrium and uterus measured by transvaginal sonography are not an effective predictor of pregnancy outcome in frozen-thawed embryo transfer cycles.

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Section: Discussionmentioning

confidence: 88%

Measurement of endometrial and uterine vascularity by transvaginal ultrasonography in predicting pregnancy outcome during frozen–thawed embryo transfer cycles

Son

Jeong

Joo

et al. 2014

J of Obstet and Gynaecol

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“…(4) The opening of 50 μm probably is not large enough for the hatching process. Large ZP opening (1/2 or 2/3 of the zona diameter) [10,34] or total removal of the ZP [35] resulted in higher delivery rates than partial opening. Whether the possibility of MZT can be reduced by enlarging the opening size or total removal of the ZP needs more clinical data.…”

Section: Discussionmentioning

confidence: 97%

Effect of the site of assisted hatching on vitrified-warmed blastocyst transfer cycles: a prospective randomized study

Ren

Liu

Chen

et al. 2013

J Assist Reprod Genet

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Purpose To assess the effect of assisted hatching (AH) site on the clinical outcomes in vitrified-warmed blastocyst transfer cycles. Methods A total of 160 women who underwent vitrifiedwarmed blastocyst transfer cycles were randomized to either the ICM group (AH performing at the site near the inner cell mess, ICM), or the TE group (AH performing at the site opposite to the ICM). AH with laser zona drilling was performed 20 or 30 min after thawing once the ICM can be detected. Clinical pregnancy rate, implantation rate, live birth rate and the occurrence rate of monozygotic twins (MZT) pregnancy after transfer of these two groups were compared. Results No significant difference was found in the clinical pregnancy rate (63.8 % vs. 67.5 %), implantation rate (51.7 % vs. 53.6 %) and live birth rate (57.5 % vs. 62.5 %) between the ICM group and the TE group. The occurrence rate of MZT was comparable between the two groups (3.9 % vs. 5.6 %). Conclusions The site of assisted hatching has no influence on the implantation, pregnancy and live birth rate in human vitrified-warmed blastocyst transfer cycles.

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“…In conclusion, sucrose has been used for several decades in the cryopreservation of embryos, but when the artificial shrinkage metnhod was used, the survival rate of mouse blastocysts was higher in the absence of sucrose than in the presence of sucrose [ 39 ]. In our clinic, we have used vitrification solution without sucrose in the cryopreservation of human blastocysts, and the outcomes are better than when vitrification solution with sucrose is used.…”

Section: Discussionmentioning

confidence: 99%

Vitrification solution without sucrose for cryopreservation in mouse blastocysts

Joo

Lee

Jeong

et al. 2014

Clin Exp Reprod Med

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ObjectiveThis study was designed to investigate the survival rate of vitrified mouse blastocysts depending on the presence or absence of sucrose in vitrification solution.MethodsMouse two-cell embryos were collected and cultured to blastocysts. Two vitrification solutions were prepared. The control solution was composed of 25% glycerol, 25% ethylene glycol, and 0.5 M sucrose (G25E250.5S) containing 2.5 mL glycerol, 2.5 mL ethylene glycol, 2 mL SSS, and 0.855 g sucrose in 5 mL PB1. The experimental solution was composed of 25% glycerol and 25% ethylene glycol (G25E25) and contained 2.5 mL glycerol and 2.5 mL ethylene glycol in 5 mL PB1. Artificial shrinkage was conducted by aspirating the blastocoelic fluid using an ICSI pipette. To examine the effect of sucrose in the vitrification solution on the survival rate of mouse blastocysts, the shrunken-equilibrated blastocysts were rehydrated or vitrified after being exposed to one of the two vitrification solutions. After exposure and the vitrification-thawing process, the re-expansion rate and hatching rate were evaluated after 6 hours of in vitro culture.ResultsThe re-expansion rate of mouse blastocysts exposed to vitrification solution with and without sucrose were not different in the experimental solution (without sucrose) (98%) and the control solution (with sucrose) (92%) (p>0.05). The hatching rate was higher in the experimental solution (95%) than in the control solution (88%), but did not differ across two treatments (p>0.05). The re-expansion rate of mouse blastocysts vitrified in the control solution was 92% and 94%, respectively (p>0.05), and the hatching rate was higher in the experimental solution (90%) than in the control solution (74%) (p<0.05).ConclusionSucrose need not be added in vitrification solution for freezing of artificially shrunken mouse blastocysts.

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The effect of long zona dissection using ICSI pipettes for mechanical assisted hatching in vitrified-thawed blastocyst transfers

Abstract: LZD using ICSI pipettes for mechanical AH improves significantly complete hatching, implantation and pregnancy rates in vitrified-thawed blastocyst transfers.

Cited by 14 publications

References 13 publications

Measurement of endometrial and uterine vascularity by transvaginal ultrasonography in predicting pregnancy outcome during frozen–thawed embryo transfer cycles

Measurement of endometrial and uterine vascularity by transvaginal ultrasonography in predicting pregnancy outcome during frozen–thawed embryo transfer cycles

Effect of the site of assisted hatching on vitrified-warmed blastocyst transfer cycles: a prospective randomized study

Vitrification solution without sucrose for cryopreservation in mouse blastocysts

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