The Effect of Long Storage of Whole Blood Components on the Level of 2,3 Diphosphoglycerate and Lactic Acid in the Blood Bank, Dr. Soetomo General Hospital, Surabaya, Indonesia

Abstract

The purpose of this study was to analyze changes in levels of 2,3 DPG and lactic acid on WB storage time. This research is an observational analytical with time series design was conducted at the Clinical Pathology Installation and Blood Bank Dr. Soetomo General Hospital Surabaya in the period July -September 2020. Levels of 2,3 DPG and lactic acid were measured in 16 bags of Whole Blood components on the day 1, day 5, day 10, day 20 and day 30. Statistical analysis was performed using the Friedman test. The results were statistically significant if p <0.05. The Friedman statistical test showed that there were significant differences in levels of 2.3 DPG (p <0.001) and levels of lactic acid (p <0.001) during storage. The results showed that the level of 2,3 DPG in WB which was stored decreased according to the duration of storage. The yield of lactic acid on stored WB increased with the duration of storage. Therefore, saving WB is recommended to be given within <6 days to reduce the risk of acidosis. Further research is needed for other parameters that can affect the storage process.