The Effect of Locally Administered Anti-Growth Factor Antibodies on Neointimal Hyperplasia Formation in Expanded Polytetrafluoroethylene Grafts

Sapienza, Paolo; Marzo, Luca di; Cucina, Alessandra; Borrelli, Valeria; Mosiello, Giovanni; Basile, Ursula; Iacovitti, S.; Cavallaro, Antonino

doi:10.1016/j.avsg.2008.11.012

Cited by 8 publications

(6 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The activation of leukocytes is mediated by IL-1, IL-6, and TNF-α and permits the contact between leukocytes and the adhesion molecules on the endothelium. 12,24 Furthermore, the activated macrophages release IL-1, IL-6, and TNF-α, contributing to the maintenance of the inflammatory state created by each revascularisation procedure. In macrophage depletion studies, a reduction in inflammatory cytokines, as well as neointima formation, has been observed previously.…”

Section: Discussionmentioning

confidence: 99%

Different inflammatory cytokines release after open and endovascular reconstructions influences wound healing

Sapienza

Mingoli

Borrelli

et al. 2019

International Wound Journal

Self Cite

View full text Add to dashboard Cite

Prodromal signs of a non-healing wound after revascularisation, which might be strictly linked with impending failure of vascular reconstructions, are associated with an inflammatory response mediated by several circulating adhesion molecules, extracellular endopeptidases, and cytokines. The aim of our study was to investigate the role of selected plasma biomarkers in the prediction of both wound healing and failure of infrapopliteal vein graft or percutaneous trans-luminal angioplasty (PTA) with selective stent positioning of the superficial femoral artery (SFA) in a population affected with critical limb ischaemia. A total of 68 patients who underwent either surgical or endovascular revascularisation of the inferior limb with autologous saphenous vein infrapopliteal bypass or PTA and selective stenting of the SFA were enrolled in our study. Patients were divided into two groups according to treatment: 41 patients were included in Group 1 (open surgery) and 27 in Group 2 (endovascular procedure). Plasma and blood samples were collected on the morning of surgery and every 6 months thereafter for up to 2 years of follow-up or until an occlusion occurred of either the vein bypass graft or the vessel treated endovascularly. Fifteen age-matched healthy male volunteers were considered a reference for biological parameters. Vascular cell adhesion molecule 1 [VCAM-1]/CD106, inter-cellular adhesion molecule-1 [ICAM-1]/CD54), interleukin-1 (IL-1), interleukin-6 (IL-6), tumour necrosis factor alpha (TNF-α), and metalloproteinases (MMP)-2 and -9 plasma levels were measured with enzyme-linked immunosorbent assay (ELISA) kits. The mean observed time to heal of 54 wounds was 13 ± 4 months, with no statistically significant differences among the groups. The healing failure of the remaining wounds was strictly related to an unsuccessful open (n = 12) or endovascular (n = 8) treatment. The 2-year primary patency rate was 65% (SE = .09) in Group 1 and 52% (SE = .1) in Group 2. When compared with mean concentration values of Group 1, VCAM-1 and ICAM-1 were always significantly higher during follow-up in patients of Group 2 (P < .05). Furthermore, in the same group, IL-6 and tumour necrosis factor alpha (TNF-α) were found to be significantly higher at 6-and 12-month (P < .05) when compared with surgically treated patients. Cox regression analysis showed that

show abstract

Section: Discussionmentioning

confidence: 99%

Different inflammatory cytokines release after open and endovascular reconstructions influences wound healing

Sapienza

Mingoli

Borrelli

et al. 2019

International Wound Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…Grafts coated with VEGF receptor-2 antibodies have also shown promise, leading to increased adhesion of VEGF receptor-2-expressing cells in in vitro studies ( Liebler et al, 2016 ). Sapienza et al (2009) coated the luminal surface of grafts with antibodies against endogenous FGF, PDGF, and TGF-β1. The growth factors bind to these antibodies, which prevents them from activating local growth factor receptors and stimulating SMC proliferation.…”

Section: Targeted Delivery In Vascular Graftsmentioning

confidence: 99%

“…The growth factors bind to these antibodies, which prevents them from activating local growth factor receptors and stimulating SMC proliferation. This resulted in less intimal hyperplasia in a 4 weeks pig study ( Sapienza et al, 2009 ), though longer term studies are needed to ensure that the growth factor inhibition does not harm the endothelium. Additional approaches for enhancing EC adhesion on synthetic grafts are reviewed in ( Melchiorri et al, 2013 ; Ren et al, 2015 ).…”

Section: Targeted Delivery In Vascular Graftsmentioning

confidence: 99%

Targeted Delivery of Bioactive Molecules for Vascular Intervention and Tissue Engineering

et al. 2018

View full text Add to dashboard Cite

Cardiovascular diseases are the leading cause of death in the United States. Treatment often requires surgical interventions to re-open occluded vessels, bypass severe occlusions, or stabilize aneurysms. Despite the short-term success of such interventions, many ultimately fail due to thrombosis or restenosis (following stent placement), or incomplete healing (such as after aneurysm coil placement). Bioactive molecules capable of modulating host tissue responses and preventing these complications have been identified, but systemic delivery is often harmful or ineffective. This review discusses the use of localized bioactive molecule delivery methods to enhance the long-term success of vascular interventions, such as drug-eluting stents and aneurysm coils, as well as nanoparticles for targeted molecule delivery. Vascular grafts in particular have poor patency in small diameter, high flow applications, such as coronary artery bypass grafting (CABG). Grafts fabricated from a variety of approaches may benefit from bioactive molecule incorporation to improve patency. Tissue engineering is an especially promising approach for vascular graft fabrication that may be conducive to incorporation of drugs or growth factors. Overall, localized and targeted delivery of bioactive molecules has shown promise for improving the outcomes of vascular interventions, with technologies such as drug-eluting stents showing excellent clinical success. However, many targeted vascular drug delivery systems have yet to reach the clinic. There is still a need to better optimize bioactive molecule release kinetics and identify synergistic biomolecule combinations before the clinical impact of these technologies can be realized.

show abstract

“…Endothelial cells (ECs) in the ePTFE graft neointima originated in three ways: migration of the host endothelial cells from the two anastomoses, sedimentation of the endothelial cells or progenitor cells from the circulation and growth of the vasa vasorum into the vessel wall. 19 As compared with the control group, capillary density was less in the graft wall in the radiotherapy group. This may have resulted from damage to the vasa vasorum in-growth caused by radiation.…”

Section: Discussionmentioning

confidence: 78%

High-dose Preoperative Fractionated Radiotherapy Does not Affect the Patency and Healing of ePTFE Vascular Prosthesis after Replacement of Canine Abdominal Aorta and Inferior Vena Cava

Guo

Shu

Zhou³

et al. 2012

European Journal of Vascular and Endovascular Surgery

View full text Add to dashboard Cite

show abstract

The Effect of Locally Administered Anti-Growth Factor Antibodies on Neointimal Hyperplasia Formation in Expanded Polytetrafluoroethylene Grafts

Cited by 8 publications

References 28 publications

Different inflammatory cytokines release after open and endovascular reconstructions influences wound healing

Different inflammatory cytokines release after open and endovascular reconstructions influences wound healing

Targeted Delivery of Bioactive Molecules for Vascular Intervention and Tissue Engineering

High-dose Preoperative Fractionated Radiotherapy Does not Affect the Patency and Healing of ePTFE Vascular Prosthesis after Replacement of Canine Abdominal Aorta and Inferior Vena Cava

Contact Info

Product

Resources

About