The effect of lipopolysaccharide on anti-inflammatory and pro-inflammatory cytokines production of human amniotic epithelial cells

Motedayyen, Hossein; Fathi, Farshid; Fasihi-Ramandi, Mahdi; Taheri, Ramezan Ali

doi:10.1016/j.repbio.2018.09.005

Cited by 15 publications

(14 citation statements)

References 26 publications

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“…It has been reported that IL4 can inhibit apoptosis of liver cancer cells, and blockage of the IL4/IL4R/STAT6 axis can promote apoptosis of Hodgkin lymphoma cells [20]. However, IL4 may also be involved in the disease as a pro-inflammatory factor [21]. The expression level of IL4 is high in kidney tissue with acute kidney injury [22].…”

Section: Discussionmentioning

confidence: 99%

Anti-apoptosis mechanism of triptolide based on network pharmacology in focal segmental glomerulosclerosis rats

Jiang

Song

et al. 2020

Bioscience Reports

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Triptolide (TPL), the active component of Tripterygium wilfordii, exhibits anti-cancer and antioxidant functions. We aimed to explore the anti-apoptosis mechanism of TPL based on network pharmacology and in vivo and in vitro research validation using a rat model of focal segmental glomerulosclerosis (FSGS). The chemical structures and pharmacological activities of the compounds reported in T. wilfordii were determined and used to perform the network pharmacology analysis. The Traditional Chinese Medicine Systems Pharmacology Database (TCMSP) was then used to identify the network targets for 16 compounds from Tripterygium wilfordii. Our results showed that 47 overlapping genes obtained from the GeneCards and OMIM databases were involved in the occurrence and development of FSGS and used to construct the protein–protein interaction (PPI) network using the STRING database. Hub genes were identified via the MCODE plug-in of the Cytoscape software. IL4 was the target gene of TPL in FSGS and was mainly enriched in the cell apoptosis term and p53 signaling pathway, according to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses. TPL inhibited FSGS-induced cell apoptosis in rats and regulated IL4, nephrin, podocin, and p53 protein levels via using CCK8, TUNEL, and Western blot assays. The effects of IL4 overexpression, including inhibition of cell viability and promotion of apoptosis, were reversed by TPL. TPL treatment increased the expression of nephrin and podocin and decreased p53 expression in rat podocytes. In conclusion, TPL inhibited podocyte apoptosis by targeting IL4 to alleviate kidney injury in FSGS rats.

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Section: Discussionmentioning

confidence: 99%

Anti-apoptosis mechanism of triptolide based on network pharmacology in focal segmental glomerulosclerosis rats

Jiang

Song

et al. 2020

Bioscience Reports

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“…These cells have been already isolated from full-term human placenta obtained from healthy women (15). Approximately, after 24 h of in-cubation (5% CO2, 37℃, 90% humidity) the adherent cells were trypsinized and plated at diminishing numbers on 24-well plates (4×10 5 , 2×10 5 , 8×10 4 and 4×10 4 cell/well) for further coculture with neutrophils. Before the adding neutrophils, HAECs were allowed to adhere to the plate for 16 to 18 h.…”

Section: Preparation Of Haecsmentioning

confidence: 99%

“…HAECs do not express histocompatibility antigens (HLA-A, B or DR) and costimulatory molecules but express immunoregulatory molecules like HLA-G and CD95, so they are poorly immunogenic (4). Additionally, HAECs have anti-inflammatory properties, they possess inhibitory capacity toward activated inflammatory cells and directed the ongoing immune responses to a more immunoregulatory and tolerogenic condition (5).…”

Section: Introductionmentioning

confidence: 99%

Human Amniotic Epithelial Cells Affect the Functions of Neutrophils

Alipour

Motedayyen

Sereshki³

et al. 2020

IJSC

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Background and Objectives: As a stem cell group, Human amniotic epithelial cells (HAECs) have numerous advantages over their embryonic and adult counterparts for therapeutic utility. They are closer to clinical applications compared to other stem cell types. Additionally, the anti-inflammatory and immunoregulatory properties of HAECs toward several immune cells have been shown previously. Nevertheless, despite the ever-increasing importance of neutrophils in the immune and non-immune processes, a few studies investigated the interaction of neutrophils and HAECs. To increase the current knowledge of HAECs immunology which is necessary for optimizing their future clinical applications, here we explored the effect of HAECs on two chief neutrophil functions; respiratory burst and phagocytosis. Methods and Results: Freshly isolated human blood neutrophils were co-cultured with different number of HAECs for about 24 or 48 hours, then the oxidative burst and phagocytosis of stimulated neutrophils were assessed and compared. The results demonstrated a substantial elevation in the phagocytosis percentage, conversely a significant reduction in the oxidative burst of HAECs-cocultured neutrophils. These effects were dose-dependent, but did not show similar patterns. Likewise, the elongation of coculture period inversely influenced the HAECs-induced effects on the two neutrophil functions. Conclusions: The present study, for the first time, investigated the HAECs-mediated effects on the two main neutrophil functions. The findings suggest that HAECs by enhancement of phagocytic ability and simultaneously, attenuation of oxidative burst capacity of neutrophils protect the fetus from both microbial treats and oxidative stress and their consequent inflammation; thus corroborate the current anti-inflammatory vision of HAECs.

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“…Production of LPS caused by periodontal health problems has been shown to induce an increase in proinflammatory cytokines that in turn cause neuroinflammation and cognitive impairment, which ultimately may result in diseases such as dementia and AD [5,24]. A possible explanation for this apparent ambiguity may be that LPS induces production of both pro-and antiinflammatory cytokines, as it has been previously demonstrated in vitro [28], in order to keep an immune balance. In addition, certain cells may be more susceptible to the deleterious effect of LPS than others [29].…”

Section: Discussionmentioning

confidence: 98%

Consecutive intra-gingival injections of lipopolysaccharide and butyric acid to mice induce abnormal behavior and changes in cytokine concentrations

Tsukahara

Toyoda

Kawase

et al. 2020

J Neuroinflammation

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Background Periodontopathic bacteria such as Porphyromonas gingivalis produce several metabolites, including lipopolysaccharide (LPS) and n-butyric acid (BA). Past work suggested that periodontal infection may cause cognitive impairment in mice. Aims To elucidate the mechanisms by which metabolites such as LPS and BA, resulting from Porphyromonas gingivalis activity, induce immunological and physiological abnormalities in mice. Methods In the present work, 28 male ICR mice were placed in an open-field arena and the total distance (cm/600 s) they covered was recorded. Based on their moving distances, mice were divided into 4 groups (n = 7) and injected the following substances into their gingival tissues for 32 consecutive days: saline (C), 5 mmol/L of BA (B), 1 μg/mouse of LPS (L), and BA-LPS (BL) solutions. Distances covered by mice were also measured on days 14 and 21, with their habituation scores considered as “(moving distance on day 14 or 21)/(moving distance on day 0)”. Afterwards, mice were dissected, and hippocampal gene expression and the concentrations of short-chain fatty acids, neurotransmitters and cytokines in their blood plasma and brains were analyzed. In addition, mouse brain and liver tissues were fixed and visually assessed for histopathological abnormalities. Results Group BL had significantly higher habituation scores than C and B on day 14. LPS induced higher habituation scores on day 21. LPS induced significant decreases in the mRNA levels of interleukin (IL)-6 and brain-derived neurotrophic factors, and an increase in neurotrophic tyrosine kinase receptor type 2. In both plasma and brain, LPS induced a significant acetate increase. Moreover, LPS significantly increased acetylcholine in brain. In plasma alone, LPS and BA significantly decreased monocyte chemoattractant protein 1 (MCP-1). However, while LPS significantly decreased tyrosine, BA significantly increased it. Lastly, LPS significantly decreased IL-6 and tumor necrosis factor in plasma. No histopathological abnormalities were detected in liver or brain tissues of mice. Conclusion We showed that injections of LPS and/or BA induced mice to move seemingly tireless and that both LPS and BA injections strongly induced a reduction of MCP-1 in blood plasma. We concluded that LPS and BA may have been crucial to induce and/or aggravate abnormal behavior in mice.

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The effect of lipopolysaccharide on anti-inflammatory and pro-inflammatory cytokines production of human amniotic epithelial cells

Cited by 15 publications

References 26 publications

Anti-apoptosis mechanism of triptolide based on network pharmacology in focal segmental glomerulosclerosis rats

Anti-apoptosis mechanism of triptolide based on network pharmacology in focal segmental glomerulosclerosis rats

Human Amniotic Epithelial Cells Affect the Functions of Neutrophils

Consecutive intra-gingival injections of lipopolysaccharide and butyric acid to mice induce abnormal behavior and changes in cytokine concentrations

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