The effect of lipid phase on liposome stability upon exposure to the mechanical stress

Doskocz, Joanna; Dałek, Paulina; Foryś, Aleksander; Trzebicka, Barbara; Przybyło, Magdalena; Mesarec, Luka; Iglič, Aleš; Langner, Marek

doi:10.1016/j.bbamem.2020.183361

Cited by 23 publications

(23 citation statements)

References 53 publications

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“…The tubular liposome is formed at the pore opening (on the cis side) and the final vesicle is formed at the exit of the pore [ 63 , 82 ].…”

Section: Resultsmentioning

confidence: 99%

“…The obtained MLV suspension was extruded using an automated mechanical extruder (Lipid Systems Ltd., Wrocław, Poland) to produce a population of the unilamellar vesicles ( Figure 1 ) [ 77 ]. As described elsewhere, the extrusion process was performed using the device capable of maintaining a constant value of the volumetric flow of the liposome suspension through the filter and continuously monitoring the extrusion force [ 63 ]. The volumetric flux ( J V ) across the polycarbonate filter was calculated according to the formula:

where

is the velocity of the piston in the syringe, which was set before each measurement, whereas R S and R F stand for the syringe and filter radii, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…In previous work [ 63 ], it has been shown that the extrusion force depends on measurement conditions such as the volumetric flux of the liposome suspension through pores, temperature, pore diameter, and concentration of lipids in the suspension. In order to determine the effect of lipid composition of the lipid bilayer or test the proposed mechanisms of the liposome formation process, the experimental conditions had been fixed, specifically, the lipid concentration was set to 10 mg/mL, the volumetric flux was maintained at 0.48 (mL∙min −1 ∙cm −2 ), and pores in the filter had diameters equal to 0.1 µm if not stated otherwise.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, we have introduced a measuring technique, easy to implement into industrial settings, for the determination of the mechanical properties of a lipid bilayer [ 63 ]. The method is based on the measurement of the force needed to support the predetermined volumetric flow through the nanopores of the extrusion membrane.…”

Section: Introductionmentioning

confidence: 99%

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The Elucidation of the Molecular Mechanism of the Extrusion Process

et al. 2021

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Extrusion is a popular method for producing homogenous population of unilamellar liposomes. The technique relies on forcing a lipid suspension through cylindrical pores in a polycarbonate membrane. The quantification of the extrusion and/or recalibration processes make possible the acquisition of experimental data, which can be correlated with the mechanical properties of the lipid bilayer. In this work, the force needed for the extrusion process was correlated with the mechanical properties of a lipid bilayer derived from other experiments. Measurements were performed using a home-made dedicated device capable of maintaining a stable volumetric flux of a liposome suspension through well-defined pores and to continuously measure the extrusion force. Based on the obtained results, the correlation between the lipid bilayer bending rigidity and extrusion force was derived. Specifically, it was found that the bending rigidity of liposomes formed from well-defined lipid mixtures agrees with data obtained by others using flicker-noise spectroscopy or micromanipulation. The other issue addressed in the presented studies was the identification of molecular mechanisms leading to the formation of unilamellar vesicles in the extrusion process. Finally, it was demonstrated that during the extrusion, lipids are not exchanged between vesicles, i.e., vesicles can divide but no membrane fusion or lipid exchange between bilayers was detected.

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“…The tubular liposome is formed at the pore opening (on the cis side) and the final vesicle is formed at the exit of the pore [ 63 , 82 ].…”

Section: Resultsmentioning

confidence: 99%

where

is the velocity of the piston in the syringe, which was set before each measurement, whereas R S and R F stand for the syringe and filter radii, respectively.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Elucidation of the Molecular Mechanism of the Extrusion Process

et al. 2021

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“…The use of NCAMs for various microfluidic devices and distribution of pores is well‐known and has been reported previously (Vitarelli Jr et al., 2011). Previous reports with membrane‐based isolation of vesicles have also reported membrane‐bending causing different types of vesicles to pass through the membrane (Doskocz et al., 2020). Therefore, with the reported pore size distributions and past reports there are many likely reasons for observing some EVs larger than the 200 nm nominal size.…”

Section: Discussionmentioning

confidence: 97%

Cross‐flow microfiltration for isolation, selective capture and release of liposarcoma extracellular vesicles

Casadei

Choudhury

Sarchet

et al. 2021

J of Extracellular Vesicle

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We present a resource‐efficient approach to fabricate and operate a micro‐nanofluidic device that uses cross‐flow filtration to isolate and capture liposarcoma derived extracellular vesicles (EVs). The isolated extracellular vesicles were captured using EV‐specific protein markers to obtain vesicle enriched media, which was then eluted for further analysis. Therefore, the micro‐nanofluidic device integrates the unit operations of size‐based separation with CD63 antibody immunoaffinity‐based capture of extracellular vesicles in the same device to evaluate EV‐cargo content for liposarcoma. The eluted media collected showed ∼76% extracellular vesicle recovery from the liposarcoma cell conditioned media and ∼32% extracellular vesicle recovery from dedifferentiated liposarcoma patient serum when compared against state‐of‐art extracellular vesicle isolation and subsequent quantification by ultracentrifugation. The results reported here also show a five‐fold increase in amount of critical liposarcoma‐relevant extracellular vesicle cargo obtained in 30 min presenting a significant advance over existing state‐of‐art.

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Injectable Liposome‐Loaded Hydrogel Formulations with Controlled Release of Curcumin and α‐Tocopherol for Dental Tissue Engineering

Atila,

Dalgic,

Krzemińska

et al. 2024

Adv Healthcare Materials

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An injectable hydrogel formulation was developed utilizing low‐ and high‐molecular‐weight chitosan (LCH and HCH) incorporated with curcumin and α‐tocopherol‐loaded liposomes (Lip/Cur+Toc). Cur and Toc releases were delayed within the hydrogels. The injectability of hydrogels was proved via rheological analyses. In vitro studies were conducted using human dental pulp stem cells (hDPSCs) and human gingival fibroblasts (hGFs) to examine the biological performance of the hydrogels towards endodontics and periodontics, respectively. The viability of hDPSCs treated with the hydrogels with Lip/Cur+Toc was the highest till day 14, compared to the neat hydrogels. During odontogenic differentiation tests, ALP activity of hDPSCs was induced in the Cur‐containing groups. Biomineralization was enhanced mostly with Lip/Cur+Toc incorporation. The viability of hGFs was the highest in HCH combined with Lip/Cur+Toc while wound healing occurred almost 100% in both (Lip/Cur+Toc@LCH and Lip/Cur+Toc@HCH) after 2 days. Antioxidant activity of Lip/Cur+Toc@LCH on hGFs was significantly the highest among the groups. Antimicrobial tests demonstrated that Lip/Cur+Toc@LCH was more effective against Escherichia coli whereas so was Lip/Cur+Toc@HCH against Staphylococcus aureus. The antimicrobial mechanism of the hydrogels was investigated for the first time through various computational models. LCH and HCH loaded with Lip/Cur+Toc were promising candidates with multi‐functional features for endodontics and periodontics.This article is protected by copyright. All rights reserved

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The effect of lipid phase on liposome stability upon exposure to the mechanical stress

Cited by 23 publications

References 53 publications

The Elucidation of the Molecular Mechanism of the Extrusion Process

The Elucidation of the Molecular Mechanism of the Extrusion Process

Cross‐flow microfiltration for isolation, selective capture and release of liposarcoma extracellular vesicles

Injectable Liposome‐Loaded Hydrogel Formulations with Controlled Release of Curcumin and α‐Tocopherol for Dental Tissue Engineering

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