The Effect of Light Quality on the Carbon Metabolism and Extracellular Release of Chlamydomonas Reinhardtii Dangeard1

Brown, T. J.; Geen, G. H.

doi:10.1111/j.0022-3646.1974.00213.x

“…We compared starch levels in wild type CC125 cells (WT) exposed to white and red light, and found that red light favored starch accumulation, in agreement with prior work (16). Interestingly, when low fluence blue light was superimposed on red light, the beneficial effect of red light was lost and cells accumulated starch levels similar to those of cells exposed to white light (Supplementary Fig.…”

Section: The Phot Mutant Is a Starch Hyperaccumulatorsupporting

confidence: 86%

Phototropin connects blue light perception to starch metabolism in green algae

Petroutsos,

Yuan,

Iannetta

et al. 2024

Preprint

0

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In photosynthetic organisms light acts as an environmental signal to control their development and physiology, and as energy source to drive the conversion of CO2 into carbohydrates used for growth or storage. The main storage carbohydrate in green algae is starch, which accumulates during the day and is broken down at night to meet cellular energy demands. The signalling role of light quality in the regulation of starch accumulation remains unexplored. Here, we report that in the model green alga Chlamydomonas reinhardtii blue light perceived by the photoreceptor PHOTOTROPIN causes dephosphorylation of the PHOTOTROPIN-MEDIATED SIGNALLING KINASE 1 that then suppresses starch accumulation by inhibiting the expression of GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE. Our results provide an in-depth view of how photoreceptor-mediated signalling controls microalgal carbon metabolism.

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“…We compared starch levels in wild type CC125 cells (WT) exposed to white and red light, and found that red light favored starch accumulation, in agreement with prior work (16). Interestingly, when low fluence blue light was superimposed on red light, the beneficial effect of red light was lost and cells accumulated starch levels similar to those of cells exposed to white light ( Fig.…”

Section: Introductionsupporting

confidence: 87%

“…In this work we report the discovery of a novel light-signalling pathway linking blue-light perception by PHOT with starch accumulation in Chlamydomonas. Our data solve a four-decadeslong question about the reasons for starch accumulation in green algae predominantly under red light (16). We showed that PHOT controls the phosphorylation state of a single serine residue S120 of a newly identified kinase, termed PMSK1, which in turn transduces the signal to activate transcription of GAP1 (Fig.…”

Section: Discussionmentioning

confidence: 54%

Phototropin connects blue light perception to starch metabolism in green algae

Yuan,

Iannetta,

Kim

et al. 2024

Preprint

1

0

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In photosynthetic organisms light acts as an environmental signal to control their development and physiology, and as energy source to drive the conversion of CO2 into carbohydrates used for growth or storage. The main storage carbohydrate in green algae is starch, which accumulates during the day and is broken down at night to meet cellular energy demands. The signalling role of light quality in the regulation of starch accumulation remains unexplored. Here, we report that in the model green alga Chlamydomonas reinhardtii blue light perceived by the photoreceptor PHOTOTROPIN causes dephosphorylation of the PHOTOTROPIN-MEDIATED SIGNALLING KINASE 1 that then suppresses starch accumulation by inhibiting the expression of GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE. Our results provide an in-depth view of how photoreceptor-mediated signalling controls microalgal carbon metabolism.

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“…Phototroph-heterotroph communities are pervasive in soils and aquatic environments, where these two metabolic strategies are often found in tight symbioses. 11 , 17 , 55 , 60 , 63 Phototroph-heterotroph symbioses typically arise from phototrophs fixing CO 2 and excreting organic compounds 3 , 5 , 10 , 18 , 48 , 64 that can be subsequently utilized by heterotrophs. However, this is not the only source of organic carbon for heterotrophic bacteria, because they can also utilize nutrients from decaying organic matter.…”

Section: Introductionmentioning

confidence: 99%