The effect of light on embryos and embryo culture

Pomeroy, Kimball O.; Reed, Michael L.

doi:10.1017/cbo9781107294295.008

Cited by 12 publications

(25 citation statements)

References 41 publications

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“…In one study, photooxidation of media components (riboflavin, tryptophan, and tyrosine) resulted in the production of toxic hydrogen peroxide. The addition of N-2- Pomeroy and Reed, 2013 hydroxyethylpiperazine-N′-2-ethanesulfonic acid (HEPES) to the culture medium RPMI 1640 markedly increased the production of cytotoxic products during exposure of the medium to visible light (Zigler et al, 1985).…”

Section: Light Toxicity Tissue Cultures and Nonprimate Embryosmentioning

confidence: 99%

“…In fact, there were few to no standards or laboratory prototypes available, nor was there consensus as to what constituted the 'ideal' laboratory environment. With regard to human clinical IVF, there is one early publication discussing changes made in the laboratory design, prospectively, to improve the laboratory environment; two Pomeroy and Reed, 2013 parameters were considered, oxygen tension and lighting in the laboratory (Noda et al, 1994). While this study did not follow through to embryo transfer and gestational parameters, supernumerary spare embryos were allowed to develop under 1) low oxygen conditions (5%) and 2) low intensity lighting (20 and 100 lux, as measured from ambient and microscope lights, respectively).…”

Section: Light Exposure In the Human Ivf Laboratorymentioning

confidence: 99%

“…mitochondrial function, where the potential damaging nature of these molecules is balanced by the inherent antioxidant capacity of the cells. When this balance is interrupted, an overabundance of the more potent radicals (hydroxyl and peroxide Pomeroy and Reed, 2013 compounds) can damage cells, specifically targeting lipids and DNA.…”

Section: Reactive Oxygen Speciesmentioning

confidence: 99%

“…An additional important and often overlooked component of the culture system is the quality of the oil used to overlay culture medium; a commercial oil product may arrive sterile from the manufacturer with a valid certificate of Pomeroy and Reed, 2013 analysis, but improper transportation temperature, exposure to sunlight and/or visible light, or improper storage may result in adulteration of the oil. Peroxides can form in a cascade reaction as a result of exposure to light and/or heat, resulting in transfer of water soluble contaminants to the culture drops (Otsuki et al, 2009(Otsuki et al, , 2007Provo and Herr, 1998).…”

Section: Reactive Oxygen Speciesmentioning

confidence: 99%

“…Further, the oocyte and the embryo may not have retained a Pomeroy and Reed, 2013 functional system to either protect or repair potential light damage during these steps of in vitro fertilization and so it is possible that irreparable harm is being done.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Light on Embryos and Embryo Culture

Pomeroy¹,

Reed²

2012

Journal of Reproductive and Stem Cell Biotechnology

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In oviparous animals, the egg hatches outside of the body and is exposed to light; in some cases throughout the development of the fetus. In mammals, fertilization and the growth of embryos in vivo occurs in the dark but in human IVF, these embryos are exposed to variable light sources and intensities. Light can affect embryonic development in some species via either a direct toxic effect on the embryo, or indirectly via photo-oxidation of components in the media or oil. Although data regarding the effect of light on human embryos is lacking, it is prudent to take appropriate steps to minimize the potential harmful effects of both ambient and microscopic light on embryos. Disclaimer:The authors declare no conflicts of interest, whether of a financial or other nature J Reprod Stem Cell Biotechnol 3(2):46-54

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Section: Light Toxicity Tissue Cultures and Nonprimate Embryosmentioning

confidence: 99%

Section: Light Exposure In the Human Ivf Laboratorymentioning

confidence: 99%

Section: Reactive Oxygen Speciesmentioning

confidence: 99%

Section: Reactive Oxygen Speciesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Effect of Light on Embryos and Embryo Culture

Pomeroy¹,

Reed²

2012

Journal of Reproductive and Stem Cell Biotechnology

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Effect of blue light‐emitting diode light and antioxidant potential in a somatic cell

Bapary

Takano

Soma

et al. 2019

Cell Biology International

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Light is an indispensable part of routine laboratory work in which conventional light is generally used. Light-emitting diodes (LEDs) have come to replace conventional light, and thus could be a potent target in biomedical studies. Since blue light is a major component of visible light wavelength, in this study, using a somatic cell from the African green monkey kidney, we assessed the possible consequences of the blue spectra of LED light in future animal experiments and proposed a potent mitigation against light-induced damage. COS-7 cells were exposed to blue LED light (450 nm) and the growth and deoxyribonucleic acid (DNA) damage were assessed at different exposure times. A higher suppression in cell growth and viability was observed under a longer period of blue LED light exposure. The number of apoptotic cells increased as the light exposure time was prolonged. Reactive oxygen species (ROS) generation was also elevated in accordance to the extension of light exposure time. A comparison with dark-maintained cells revealed that the upregulation of ROS by blue LED light plays a significant role in causing cellular dysfunction in DNA in a time-dependent manner. In turn, antioxidant treatment has been shown to improve cell growth and viability under blue LED light conditions. This indicates that antioxidants have potential against blue LED light-induced somatic cell damage. It is expected that this study will contribute to the understanding of the basic mechanism of somatic cell death under visible light and maximize the beneficial use of LED light in future animal experiments.

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‘There is only one thing that is truly important in an IVF laboratory: everything’ Cairo Consensus Guidelines on IVF Culture Conditions

Group¹

2020

Reproductive BioMedicine Online

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Cairo 2018 Consensus Group* KEY MESSAGE This report presents outcomes from an international expert meeting to establish consensus guidelines on IVF culture. Topics reviewed were: embryo culture; temperature; humidity; gas control, pH; workstations; incubators; micromanipulation; handling and assessment; stasis, composition, supplementation, type of culture and storage; equipment and monitoring. More than 50 consensus guideline points were established.

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The effect of light on embryos and embryo culture

Cited by 12 publications

References 41 publications

The Effect of Light on Embryos and Embryo Culture

The Effect of Light on Embryos and Embryo Culture

Effect of blue light‐emitting diode light and antioxidant potential in a somatic cell

‘There is only one thing that is truly important in an IVF laboratory: everything’ Cairo Consensus Guidelines on IVF Culture Conditions

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