The effect of light intensity on hydrogen production by sulfur-deprived Chlamydomonas reinhardtii

Laurinavichene, Tatyana V.; Tolstygina, Irena V.; Tsygankov, Anatoly A.

doi:10.1016/j.jbiotec.2004.05.012

Cited by 116 publications

(43 citation statements)

References 9 publications

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“…Activity of the indirect pathway in H 2 production can be estimated by inhibiting residual PSII activity using DCMU (Fouchard et al, 2005;Chochois et al, 2009). Based on such a measurement, the contribution of the indirect pathway to H 2 production has been reported to be variable, depending on experimental conditions and particularly on the phase of the sulfur deprivation process (Kosourov et al, 2003;Laurinavichene et al, 2004;Fouchard et al, 2005). In our conditions, activity of the indirect pathway was about 10 times lower than that of the direct pathway under conditions of sulfur deficiency (Chochois et al, 2009).…”

Section: Resultsmentioning

confidence: 78%

Control of Hydrogen Photoproduction by the Proton Gradient Generated by Cyclic Electron Flow in Chlamydomonas reinhardtii

et al. 2011

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Section: Resultsmentioning

confidence: 78%

Control of Hydrogen Photoproduction by the Proton Gradient Generated by Cyclic Electron Flow in Chlamydomonas reinhardtii

et al. 2011

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“…This indicates that electrons provided by wateroxidation at PSII do not contribute to linear electron transport towards the hydrogenase, and moreover, it raises the question about the electron source for the comparably low, but still significant H 2 evolution activity of N-depleted algal cells. H 2 production by S-deficient Chlamydomonas cultures are reduced in the presence of DCMU, though to varying extents (Fouchard et al 2005;Hemschemeier et al 2008a) and depending on the time point within the sulfur deprivation experiment (Laurinavichene et al 2004;Hemschemeier et al 2008a). It has been proposed that residual H 2 -producing activity of DCMU-treated S-starved cells depends on non-photochemical PQ-reduction by stromal reductants originating from starch breakdown, which, however, is slow in N-deprived cultures.…”

Section: Discussionmentioning

confidence: 99%

Nitrogen deprivation results in photosynthetic hydrogen production in Chlamydomonas reinhardtii

Philipps

Happe

Hemschemeier

2011

Planta

145

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The unicellular green alga Chlamydomonas reinhardtii is able to use photosynthetically provided electrons for the production of molecular hydrogen by an [FeFe]-hydrogenase HYD1 accepting electrons from ferredoxin PetF. Despite the severe sensitivity of HYD1 towards oxygen, a sustained and relatively high photosynthetic hydrogen evolution capacity is established in C. reinhardtii cultures when deprived of sulfur. One of the major electron sources for proton reduction under this condition is the oxidation of starch and subsequent non-photochemical transfer of electrons to the plastoquinone pool. Here we report on the induction of photosynthetic hydrogen production by Chlamydomonas upon nitrogen starvation, a nutritional condition known to trigger the accumulation of large deposits of starch and lipids in the green alga. Photochemistry of photosystem II initially remained on a higher level in nitrogen-starved cells, resulting in a 2-day delay of the onset of hydrogen production compared with sulfur-deprived cells. Furthermore, though nitrogen-depleted cells accumulated large amounts of starch, both hydrogen yields and the extent of starch degradation were significantly lower than upon sulfur deficiency. Starch breakdown rates in nitrogen or sulfur-starved cultures transferred to darkness were comparable in both nutritional conditions. Methyl viologen treatment of illuminated cells significantly enhanced the efficiency of photosystem II photochemistry in sulfur-depleted cells, but had a minor effect on nitrogen-starved algae. Both the degradation of the cytochrome b₆ f complex which occurs in C. reinhardtii upon nitrogen starvation and lower ferredoxin amounts might create a bottleneck impeding the conversion of carbohydrate reserves into hydrogen evolution.

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“…The classical TAP medium is overloaded with all key nutrients except for acetate, which normally runs out in the first three days of algal growth. 18 Two variations of the TAP medium were used in experiments: TAP-S and TAP 12 . 19 The TAP-S medium was prepared by replacing sulphate salts with their chloride derivatives; it provides a sulphur-deprived environment, which is necessary for the induction of H 2 production.…”

Section: Algae and Algal Growth Mediamentioning

confidence: 99%

Process and reactor design for biophotolytic hydrogen production

Tamburic

Dechatiwongse

Zemichael

et al. 2013

Phys. Chem. Chem. Phys.

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The green alga Chlamydomonas reinhardtii has the ability to produce molecular hydrogen (H 2 ), a clean and renewable fuel, through the biophotolysis of water under sulphur-deprived anaerobic conditions. The aim of this study was to advance the development of a practical and scalable biophotolytic H 2 production process. Experiments were carried out using a purpose-built flat-plate photobioreactor, designed to facilitate green algal H 2 production at the laboratory scale and equipped with a membrane-inlet mass spectrometry system to accurately measure H 2 production rates in real time. The nutrient control method of sulphur deprivation was used to achieve spontaneous H 2 production following algal growth. Sulphur dilution and sulphur feed techniques were used to extend algal lifetime in order to increase the duration of H 2 production. The sulphur dilution technique proved effective at encouraging cyclic H 2 production, resulting in alternating Chlamydomonas reinhardtii recovery and H 2 production stages. The sulphur feed technique enabled photobioreactor operation in chemostat mode, resulting in a small improvement in H 2 production duration. A conceptual design for a large-scale photobioreactor was proposed based on these experimental results. This photobioreactor has the capacity to enable continuous and economical H 2 and biomass production using green algae.The success of these complementary approaches demonstrate that engineering advances can lead to improvements in the scalability and affordability of biophotolytic H 2 production, giving increased confidence that H 2 can fulfil its potential as a sustainable fuel of the future.

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The effect of light intensity on hydrogen production by sulfur-deprived Chlamydomonas reinhardtii

Cited by 116 publications

References 9 publications

Control of Hydrogen Photoproduction by the Proton Gradient Generated by Cyclic Electron Flow in Chlamydomonas reinhardtii

Control of Hydrogen Photoproduction by the Proton Gradient Generated by Cyclic Electron Flow in Chlamydomonas reinhardtii

Nitrogen deprivation results in photosynthetic hydrogen production in Chlamydomonas reinhardtii

Process and reactor design for biophotolytic hydrogen production

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