The effect of light exposure on the cleavage rate and implantation capacity of preimplantation murine embryos

Bognár, Zita; Csabai, Timea; Pállinger, Éva; Balassa, Tímea; Farkas, Nelli; Schmidt, J.; Gorgey, Eva; Berta, Gergely; Szekeres-Barthó, Júlia; Bódis, József

doi:10.1016/j.jri.2019.02.003

Cited by 29 publications

(18 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Because of the significant (approximately 60%) embryo loss, we need to eliminate or at least minimize the harmful effects during the manipulation. Bognar et al [20], showed that light exposure significantly reduced the implantation capacity of mouse embryos, and that the harmful effect was related to the wavelength rather than to the brightness of the light. Previous studies have shown that not only UV radiation but also visible light is toxic to mammalian cells.…”

Section: Discussionmentioning

confidence: 99%

How to Reduce the Potential Harmful Effects of Light on Blastocyst Development during IVF

et al. 2020

View full text Add to dashboard Cite

Highlights of the Study • The aim of this study was to reduce the potential harmful effects of light exposure during IVF and ICSI. • The fertilization rate was significantly higher in light-protected ICSI cycles. • Blastocyst development rates (blastocyst/embryo) were significantly (p < 0.001) higher in lightprotected embryos. • Light protection increases the number of clinical pregnancies/transfers.

show abstract

Section: Discussionmentioning

confidence: 99%

How to Reduce the Potential Harmful Effects of Light on Blastocyst Development during IVF

et al. 2020

View full text Add to dashboard Cite

show abstract

“…An assisted reproduction technology (ART) procedure usually implies the exposure of embryos to visible light during embryo inspection and transportation. Since the in vivo environment of embryos is much darker than that in vitro, the exposure to light is an unnatural stress factor for embryos in in vitro fertilization (IVF) procedures [1]. This light exposure during embryo manipulation is harmful for embryo viability, as it directly induces many stress-related metabolic processes or activates reactions that lead to embryo apoptosis e.g., the generation of intracytoplasmic reactive oxygen species [2].…”

Section: Introductionmentioning

confidence: 99%

Light-induced injury in mouse embryos revealed by single-cell RNA sequencing

Liu

Chen

et al. 2019

Biol Res

View full text Add to dashboard Cite

Background Light exposure is a common stress factor in in vitro manipulation of embryos in the reproductive center. Many studies have shown the deleterious effects of high-intensity light exposure in different animal embryos. However, no transcriptomic studies have explored the light-induced injury and response in preimplantation embryos. Results Here, we adopt different time-courses and illumination intensities to treat mouse embryos at the 2-cell stage and evaluate their effects on blastulation. Meanwhile, single-cell transcriptomes from the 2-cell to blastocyst stage were analyzed after high-intensity light exposure. These data show that cells at each embryonic stage can be categorized into different light conditions. Further analyses of differentially expressed genes and GO terms revealed the light-induced injury as well as the potential repair response after high-intensity lighting. Maternal-to-zygote transition is also affected by the failure to remove maternal RNAs and deactivate zygotic genome expression. Conclusion Our work revealed an integrated response to high-intensity lighting, involving morphological changes, long-lasting injury effects, and intracellular damage repair mechanisms.

show abstract

“…Taurine included in fertilization and embryo culture medium [ 75 ] seems to act as an antioxidant [ 274 ]. Although mouse eggs are less sensitive to light than hamster eggs [ 273 ], a detrimental effect of light on embryonic development in the mouse is a certain possibility [ 275 ]. Since it is well known that hybrid mice are more resistant to various environmental stresses than are inbred mice, negative results obtained by experiments using hybrid mice [ 276 ] should be taken with caution.…”

Section: Effect Of Light On Eggs and Embryosmentioning

confidence: 99%

Mysteries and unsolved problems of mammalian fertilization and related topics

Yanagimachi

2022

Biology of Reproduction

View full text Add to dashboard Cite

Mammalian fertilization is a fascinating process that leads to the formation of a new individual. Eggs and sperm are complex cells that must meet at the appropriate time and position within the female reproductive tract for successful fertilization. I have been studying various aspects of mammalian fertilization over 60 years. In this review, I discuss many different aspects of mammalian fertilization, some of my laboratory’s contribution to the field, and discuss enigmas and mysteries that remain to be solved.

show abstract

The effect of light exposure on the cleavage rate and implantation capacity of preimplantation murine embryos

Cited by 29 publications

References 19 publications

How to Reduce the Potential Harmful Effects of Light on Blastocyst Development during IVF

How to Reduce the Potential Harmful Effects of Light on Blastocyst Development during IVF

Light-induced injury in mouse embryos revealed by single-cell RNA sequencing

Mysteries and unsolved problems of mammalian fertilization and related topics

Contact Info

Product

Resources

About