The effect of leukocyte-reduced platelet-rich plasma on the proliferation of autologous adipose-tissue derived mesenchymal stem cells1

Abstract

Clinical application of platelet-rich plasma (PRP) and stem cells has become more and more important in regenerative medicine during the last decade. However, differences in PRP preparations may contribute to variable PRP compositions with unpredictable effects on a cellular level. In the present study, we modified the centrifugation settings in order to provide a leukocyte-reduced PRP and evaluated the interactions between PRP and adipose-tissue derived mesenchymal stem cells (ASCs).PRP was obtained after modification of three different centrifugation settings and investigated by hemogram analysis, quantification of protein content and growth factor concentration. ASCs were cultured in serum-free α-MEM supplemented with autologous 10% or 20% leukocyte-reduced PRP. Cell cycle kinetics of ASCs were analyzed using flow cytometric analyses after 48 hours.Thrombocytes in PRP were concentrated, whereas erythrocytes, and white blood cells (WBC) were reduced, independent of centrifugation settings. Disabling the brake further reduced the number of WBCs. A higher percentage of cells in the S-phase in the presence of 20% PRP in comparison to 10% PRP and 20% fetal calf serum (FCS) advocates the proliferation stimulation of ASCs.These findings clearly demonstrate considerable differences between three PRP separation settings and assist in safeguarding the combination of leukocyte-reduced PRP and stem cells for regenerative therapies.

Adipose tissue-derived mesenchymal stem cells and platelet-rich plasma: stem cell transplantation methods that enhance stemness

“…Furthermore, various methods for manipulating PRP have been reported. For example, the platelet concentration in plasma [ 68 ], the volume of PRP transplantation, and the method of activation contribute to the effect of PRP on cell proliferation and differentiation directly. For these reasons, appropriate methods when using PRP are still controversial.…”

Effects of platelet-rich plasma on liver regeneration in CCl4-induced hepatotoxicity model

“…PRP as well as leukocyte-reduced PRP accelerates the stem cell proliferation [31] and inhibits apoptosis [32]. However, the majority of the research confirmed the suppressing cell apoptosis by PRP administration; it has also been shown that in vitro treatment of PRP led to increased apoptosis in the cells such as chondrocytes [33].…”

ACA-Technik: „stromal vascular fraction“, „platelet-rich plasma“ und Mikrofett zur körpereigenen Regeneration und Hautverjüngung

“…Die hierfür nötigen Bausteine der Reparationstriade lassen sich durch PRP (Signalmoleküle), SVF (Zellen) und Mikrofett (Matrix) darstellen. In-vitro-Studien zeigen dass die Kombination von PRP und SVF zu verstärktem Wachstum und Proliferation von ASC sowie Stimulation der Proliferation und Migration von Fibroblasten und Keratinozyten führt [8,19]. Diese Kombination ist aus unserer kürzlich publizierten Studie auch als Platelet-Rich-Stroma (PRS oder ACP SVF ) bekannt [20].…”