The effect of Lactobacillus with prebiotics on KPC-2-producing Klebsiella pneumoniae

Abstract

ObjectivesThis study investigated the inhibitory effect of Lactobacillus spp. with prebiotics against Klebsiella pneumoniae carbapenemase-2 (KPC-2)-producing Klebsiella pneumoniae using both in vitro experiments and animal models.MethodsThirty-three Lactobacillus spp. strains were confirmed by 16S rDNA sequencing, and four different PFGE genotyped KPC-2-producing K. pneumoniae strains were selected for investigation. In vitro studies, including broth microdilution assays, changes in pH values in lactobacilli cultures with different prebiotics, time-kill tests of Lactobacillus spp. against KPC-2-producing K. pneumoniae and further in vivo Lactobacillus alone or in combination with prebiotics against KPC-2-producing K. pneumoniae in an animal model, were performed.ResultsThe lower pH value of the cell-free supernatant was associated with a lower minimal inhibitory percentage of the Lactobacillus strain against KPC-2-producing K. pneumoniae. Furthermore, lactulose/isomalto-oligosaccharide/inulin and fructo-oligosaccharide can enhance the inhibitory effect of all 107 CFU/ml Lactobacillus strains against KPC001. Three Lactobacillus strains (LYC1154, LYC1322, and LYC1511) that could be persistently detected in the stool were tested for their ability to reduce the amount of KPC001 in the feces individually or in combination. A significantly better effect in reducing the amount of KPC001 was observed for the combination of three different Lactobacillus species than for each of them alone. Furthermore, their inhibitory effect was enhanced after adding lactulose or isomalto-oligosaccharide (both p < 0.05).ConclusionThis study demonstrates the inhibitory effect of probiotic Lactobacillus, including LYC1154, LYC1322, and LYC1511, with prebiotics such as lactulose or isomalto-oligosaccharide against the colonization of KPC-2-producing K. pneumoniae.

Clinical use of tigecycline may contribute to the widespread dissemination of carbapenem-resistant hypervirulent Klebsiella pneumoniae strains

“…Recent studies have reported that the anticolonization of K. pneumoniae by Lactobacillus spp. was attributed to increased acetic acid and regulation in microbiota in mice intestinal [ 29 , 34 ]. To investigate the mechanism underlying the inhibition of CR-hvKP colonization by Lactobacillus spp., we repeated the K. pneumoniae colonization experiment and replaced Lactobacillus spp.…”

Antibacterial properties and urease suppression ability of Lactobacillus inhibit the development of infectious urinary stones caused by Proteus mirabilis

“…They play a significant role in maintaining the homeostasis of this environment and the disturbance of which could affect the development of many uropathogens 15 , 39 . Many recent studies focus on the antibacterial and antibiofilm properties of natural microbiota of the urinary tract against the most common UTI pathogens such as: Escherichia coli, which cause approximately 80% of UTIs 40 , 41 , Proteus mirabilis 14 , 42 or Klebsiella pneumoniae 43 , 44 . Additionally, a lot of scientific works concentrate on the practical use of selected Lactobacillus strains to UTIs treatment.…”

Polygonatum odoratum polysaccharide attenuates lipopolysaccharide‐induced lung injury in mice by regulating gut microbiota

“…Notably, Klebsiella could disrupt the gut barrier (Nakamoto et al, 2019), which not only trigger a systemic or local inflammatory response that further altering the overall immune homeostasis of the host to exacerbate the occurrence of inflammatory lung injury (Belkaid & Hand, 2014), but also lead to a risk factor for pneumonia-induced lung injury (Liu et al, 2018). Lactobacillus, a genus of beneficial bacteria, can inhibit the growth and activity of Klebsiella (Tang et al, 2022). As a potential prebiotic-like polysaccharide, POP might promote the growth of Lactobacillus to inhibit the growth and activity of Klebsiella.…”

“…The gut microbiota was modulated by a short‐term (3‐day) POP pre‐intervention and the trend was sustained of Lactobacillus in the donor mice, which played important roles in gut microbiota for prevention in LPS‐induced injury. Lactobacillus , a genus of beneficial bacteria, can inhibit the growth and activity of Klebsiella (Tang et al, 2022 ). As a potential prebiotic‐like polysaccharide, POP might promote the growth of Lactobacillus to inhibit the growth and activity of Klebsiella .…”