The effect of Lactobacillus plantarum hydrolysates promoting VEGF production on vascular growth and hair growth of C57BL/6 mice

Abstract

Purpose: Angiogenesis is critical in various biological processes, such as blood vessel growth, fetal differentiation, wound healing, and organ regeneration. Various growth factors have been associated with vascular regeneration, including insulin-like growth factor 1 (IGF-1), transforming growth factor-β (TGF-β), and basic fibroblast growth factor (bFGF). One of the most important mediators of vascular regeneration is vascular endothelial growth factor (VEGF). VEGF is known to increase vascular permeability, induce the proliferation of endothelial cells, and stimulate capillary formation in vivo, which are core angiogenic functions. Methods: The hydrolysates of lactic acid bacteria were produced by hydrolyzing Lactobacillus plantarum with proteases, treated with MG-63 osteoblasts, and screened to obtain samples with an excellent VEGF production effect. These samples were applied to human dermal papilla cells (hDPC) to examine the correlation between cell growth and VEGF secretion. Furthermore, the hair growth rate was measured in hair growth experiments using C57BL/6 male mice. Results: The hydrolysates of the lactic acid bacteria produced in this study produced hair growth superior to the growth obtained with 5% minoxidil in hair growth experiments using C57BL/6 male mice. Conclusions: This study aims to develop a material for application to the scalp that promotes angiogenesis in the scalp and facilitates the exchange of nutrients and wastes in the follicles to promote hair growth.

Hair Growth Promotion Effect of Nelumbinis Semen Extract with High Antioxidant Activity

“…A CCK-8 assay was used to determine cell proliferation. The hDPCs were seeded at a 3 × 10 3 cells/well density in 96-well microplates for 24 h at 5% CO 2 and 37 ° C. The NS extract was diluted to 7 different concentrations (3.91, 7.81, 15.63, 31.52, 62.5, 125, and 250 ppm), and the cells were allowed to grow in 10 μ l of each NS concentration for 24 h, as described in several previous hair loss studies [ 10 , 23 , 24 ]. DMEM medium, minoxidil, and triton X-100 (Sigma) were used as a negative control (NC), positive control (PC), and blank, respectively.…”

“…After 20 min in the dark at room temperature, the absorbance of the mixture was measured at 517 nm using a 7315 UV spectrophotometer (JENWAY, Staffordshire, United Kingdom). Electron-donating ability (EDA) was calculated as described as follows: , and 250 ppm), and the cells were allowed to grow in 10 μl of each NS concentration for 24 h, as described in several previous hair loss studies [10,23,24]. DMEM medium, minoxidil, and triton X-100 (Sigma) were used as a negative control (NC), positive control (PC), and blank, respectively.…”

“…VEGF is another factor that induces growth and differentiation of hair root cells by both improving blood circulation and enhancing vascular endothelium growth [ 8 , 9 ]. Therefore, VEGF prevents hair loss by increasing HF size and hair thickness [ 10 , 11 ]. Conversely, TGF- β 1 causes apoptosis of hair follicle matrix cells and alopecia by rapidly entering the catagen phase.…”

Transdermal Composite Microneedle Composed of Mesoporous Iron Oxide Nanoraspberry and PVA for Androgenetic Alopecia Treatment

“…Especially, iron oxide nanoparticles allow them to be manipulated with externally applied magnetic field, which possess on-demand release behaviors from their cargos without any lag in response. By means of employing magnetic field, iron oxide can slightly generate heat to cause cutaneous blood flow increases as well as vasodilation, leading to the promotion of hair growth [ 20 , 21 , 22 ]. Furthermore, iron is one of the most important nutritional factors in human body, as it can help oxygen delivery as well as carbon dioxide removal from animal tissue, and has the composition of serum ferritin [ 23 ].…”

Evaluation of Serum Levels of Vascular Endothelial Growth Factor (VEGF) in Female Patients with Telogen Effluvium

“…It is thought that the growth of blood vessels is associated with hair growth and thus the VEGF is involved in hair growth. VEGF has been also reported to reduce hair loss by promoting blood circulation and the formation of new blood vessels around follicles [6].…”