Objective: To investigate whether L-carnitine (LC) inhibits eryptosis induced by uremic serum and the related mechanism. Methods: One percent erythrocyte suspension was cultured by three kinds of mediums in vitro, which was included in the control group (Group C, phosphate buffered saline [PBS]), the uremic serum group (Group U, 30% uremic serum + 70% PBS) and the LC group (Group L, 30% uremic serum + 70% PBS + 200 umol/L LC), respectively. Erythrocytes were collected at 24 and 48 h, respectively. Phosphatidylserine (PS) was estimated from Annexin-V-binding and reactive oxygen species (ROS) by flow cytometry, glutathione (GSH) was estimated from Enzyme linked immunosorbent assays (ELISA) by Microplate reader. Results: Eryptosis in Group C increased as the incubating time extended (3.43 ± 0.37 at 24 h, 4.21 ± 0.44 at 48 h). Eryptosis increased in Group U compared with Group C (6.5 1 ± 0.71 at 24 h, p50.01; 8.55 ± 0.76 at 48 h, p50.01), while decreased in Group L compared with Group U (5.80 ± 0.69 at 24 h, p50.05; 7.87 ± 0.76 at 48 h, p50.05). Meanwhile, ROS of erythrocytes increased in Group U compared with Group C (33.12 ± 1.61 versus 14.83 ± 2.22 at 24 h, p50.01; 42.06 ± 1.81 versus 20.94 ± 1.78 at 48 h, p50.01), and GSH decreased in Group U compared with Group C (25.66 ± 0.32 versus 31.27 ± 0.38 at 24 h, p50.01; 8.53 ± 0.59 versus 17.29 ± 0.54 at 48 h, p50.01). ROS of erythrocytes decreased in Group L compared with Group C (26.29 ± 1.69 at 24 h, p50.01; 36.21 ± 2.00 at 48 h, p50.01). GSH increased in Group L compared with Group U (27.54 ± 0.60 at 24 h, p50.01; 15.18 ± 0.42 at 48 h, p50.01). Conclusions: LC inhibits eryptosis induced by uremic serum, which possibly relates to oxidative stress in part.