The effect of L-arginine on intraocular pressure in the human eye

Chuman, Hideki; Chuman, Tomomi; Nao‐i, Nobuhisa; Sawada, Atsushi

doi:10.1076/0271-3683(200006)20:6;1-y;ft511

Cited by 16 publications

(16 citation statements)

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“…Therefore, the decrease of NO synthesis in patients with T-786C polymorphism could predispose to endothelial dysfunction, with consequent loss of basal vasodilator tone and reduced blood flow to ocular tissues, including the optic nerve head (Luksch et al, 2000). Furthermore, the decrease in plasma concentration of NO may be implicated in the deregulation of IOP (Chuman et al, 2000), which is known as one of the main risk factors for POAG (Kooner et al, 2008). In the overall analysis, we observed a marginal difference in the frequency of this polymorphism among cases and controls, which was not significant after adjustments for other variables of interest.…”

Section: Discussioncontrasting

confidence: 61%

Association of polymorphisms of endothelial nitric oxide synthase (eNOS) gene with the risk of primary open angle glaucoma in a Brazilian population

Silva

Rocha

Lacchini

et al. 2012

Gene

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Section: Discussioncontrasting

confidence: 61%

Association of polymorphisms of endothelial nitric oxide synthase (eNOS) gene with the risk of primary open angle glaucoma in a Brazilian population

Silva

Rocha

Lacchini

et al. 2012

Gene

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“…In the intact eye, the significance of cGMP-mediated NHE inhibition in the NPE remains to be determined. It is known, however, that NHE inhibitors reduced IOP in mouse,22 and NO donors reduce IOP in normal39–41 and glaucomatous42 animals as well an in humans 43. There is direct evidence that NO donors SNP, SNAP, and l -arginine reduce aqueous formation 8.…”

Section: Discussionmentioning

confidence: 99%

Responses of Sodium–Hydrogen Exchange to Nitric Oxide in Porcine Cultured Nonpigmented Ciliary Epithelium

Shahidullah¹,

Mandal²,

Delamere³

2009

Invest. Ophthalmol. Vis. Sci.

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Purpose To better understand how nitric oxide (NO) alters the function of the nonpigmented ciliary epithelium (NPE), studies were performed to determine the influence of NO on sodium-hydrogen exchanger (NHE) activity. Methods Cytoplasmic pH (pHi) was measured in cultured porcine NPE loaded with BCECF (2′,7′-bis(2-carboxyl)-5(6)-carboxyfluorescein-acetoxyethyl ester). Na-H exchanger (NHE) was examined by immunolocalization. Results In cells acidified by 5 minutes of exposure to 20 mM ammonium chloride, pHi recovery was partially inhibited by sodium nitroprusside (SNP), an NO donor, and l-arginine, the endogenous substrate for NO synthase. SNP and dimethyl amiloride (DMA), an NHE inhibitor, inhibited pHi recovery to a similar degree. In bicarbonate-free buffer SNP+DMA elicited no additional change in pHi recovery beyond that elicited by DMA alone. This suggests that SNP causes NHE inhibition. the SNP's effect on pHi recovery was mimicked by 8-pCPT-cGMP but suppressed by ODQ and H-8. Ouabain alone reduced pHi recovery, but SNP+ouabain caused significant further reduction. Immunolocalization studies revealed NHE1 and -4 in native and cultured NPE. Conclusions NHE1 and -4 are expressed at the NPE basolateral margin. The findings suggest the NHE is inhibited by NO which acts via a cGMP and protein kinase G signaling pathway. The NHE response does not appear to be the consequence of NO-induced Na,K-ATPase inhibition. Because NO synthases are expressed in porcine NPE, NO could act as an autocrine regulator of NHE activity. Although NHE inhibitors are known to lower intraocular pressure (IOP), further studies are needed to understand whether changes in NHE activity contribute to the IOP-lowering effect of NO donors.

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“…Our findings in the aqueous humour agree with those of other authors, 25 and with the report of decreased NOS reactivity in the aqueous outflow pathway of postmortem glaucomatous eyes. 33 Such alterations, together with pharmacological studies showing that NO donors reduce IOP elevating NO 2 2 in the anterior chamber, 24 suggest that NO might be involved in the pathogenesis or regulation of IOP in POAG.…”

Section: Discussionmentioning

confidence: 99%

Nitric oxide proxies and ocular perfusion pressure in primary open angle glaucoma

Galassi

Renieri²,

Sodi³

et al. 2004

British Journal of Ophthalmology

123

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Background: To investigate the levels of nitric oxide (NO) markers in plasma and aqueous humour of patients with primary open angle glaucoma (POAG) and their relation to ocular perfusion pressure. Methods: Cyclic guanosine monophosphate (cGMP) and nitrite (NO 2 2 ) were determined in plasma and aqueous humour of 38 patients with POAG and 46 controls. Blood pressure and IOP were measured to calculate ocular perfusion pressure (PP). Results: cGMP and NO 2 2 plasma levels were significantly decreased in glaucoma patients compared with controls (p = 0.001 v p = 0.004). In the aqueous humour of subjects with POAG, cGMP and NO 2 2 concentrations were also lower than in normal eyes (p = 0.0001 v p = 0.001). There was a linear association between cGMP in plasma and aqueous humour in glaucomas and controls (r = 0.514, p = 0.029 and r = 0.558, p = 0.004) and this relation differed in the two groups (p = 0.003). Considering glaucoma patients with controls, a positive correlation was found between cGMP and PP (r = 0.379, p = 0.01) and between NO 2 2 and PP (r = 0.339, p = 0.040). The cGMP/PP correlation was of borderline statistical significance in controls (p = 0.050), whereas it did not attain statistical significance in POAG, as well as the association between NO 2 2 and PP when glaucomas and controls were considered separately. Conclusions: The authors found alterations of NO markers in the plasma and aqueous humour of glaucoma patients. Primary or secondary impaired NO balance could alter ocular perfusion pressure.

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The effect of L-arginine on intraocular pressure in the human eye

Abstract: These findings demonstrated that L-arginine lowers IOP mainly through the formation of NO.

Cited by 16 publications

References 0 publications

Association of polymorphisms of endothelial nitric oxide synthase (eNOS) gene with the risk of primary open angle glaucoma in a Brazilian population

Association of polymorphisms of endothelial nitric oxide synthase (eNOS) gene with the risk of primary open angle glaucoma in a Brazilian population

Responses of Sodium–Hydrogen Exchange to Nitric Oxide in Porcine Cultured Nonpigmented Ciliary Epithelium

Nitric oxide proxies and ocular perfusion pressure in primary open angle glaucoma

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