The effect of L-arginine on intraocular pressure in the human eye

Chuman, Hideki; Chuman, Tomomi; Nao‐i, Nobuhisa; Sawada, Atsushi

doi:10.1076/0271-3683(200006)2061-yft511

Cited by 34 publications

(11 citation statements)

References 16 publications

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“…30 In a different study, no effect of intravenous L-arginine on IOP in humans was detected. 31 Study ethnicities, design, and analysis may account for some of the differences in findings between these two studies.…”

Section: Discussionmentioning

confidence: 99%

Effect of Nitric Oxide on Anterior Segment Physiology in Monkeys

Heyne¹,

Kiland²,

Kaufman³

et al. 2013

Invest. Ophthalmol. Vis. Sci.

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Citation: Heyne GW, Kiland JA, Kaufman PL, Gabelt BT. Effect of nitric oxide on anterior segment physiology in monkeys. Invest Ophthalmol Vis Sci. 2013;54:5103-5110. DOI: 10.1167/iovs.12-11491 PURPOSE. To determine the effect of the nitric oxide donor, sodium nitroprusside (SNP), and the nitric oxide synthase (NOS) inhibitor, L-nitro-arginine-methylester (L-NAME), on IOP, mean arterial pressure (MAP), pupil diameter (PD), refraction (Rfx), aqueous humor formation (AHF), and outflow facility (OF) in monkeys.METHODS. Monkeys were treated with single or multiple topical treatments of 500 lg SNP or L-NAME to one eye. IOP was determined by Goldmann applanation tonometry, PD with vernier calipers in room light, Rfx by Hartinger coincidence refractometry, AHF by fluorophotometry, and MAP with a blood pressure monitor. OF was determined by two-level constant pressure perfusion following anterior chamber exchange. RESULTS.Following four topical treatments with 500 lg SNP, 30 minutes apart, IOP was significantly decreased from 2 to 6 hours compared with the contralateral control with the maximum IOP reduction of 20% at 3 hours (P < 0.001). PD, Rfx, and AHF were unchanged. Effects on MAP were variable. OF after SNP exchange was significantly increased by 77% (P < 0.05) at 10 À3 M. Topical L-NAME had no effect on IOP, PD, Rfx, or MAP.CONCLUSIONS. Enhancement of nitric oxide concentration at targeted tissues in the anterior segment may be a useful approach for IOP reduction for glaucoma therapy. Additional studies are warranted before conclusions can be made regarding the effect of NOS inhibition on ocular physiology in nonhuman primates.

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Section: Discussionmentioning

confidence: 99%

Effect of Nitric Oxide on Anterior Segment Physiology in Monkeys

Heyne¹,

Kiland²,

Kaufman³

et al. 2013

Invest. Ophthalmol. Vis. Sci.

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“…3,44,45 Of these agents, NO, generated constitutively by eNOS, has a key role in maintaining the basal vasodilator tone in ocular arteries. 40,[46][47][48][49][50][51][52] The major contribution of NO to vascular tone in human ocular vasculature is demonstrated by the observation that systemic administration of an NOS inhibitor reduced blood flow in the ONH [53][54][55] and by the involvement of NO in the POAG-related vascular conditions, such as hypertension, hypotension, and vasospasms. 56 Evidence of abnormal NO production in glaucoma is also provided by genetic studies.…”

Section: Discussionmentioning

confidence: 99%

Vascular tone pathway polymorphisms in relation to primary open-angle glaucoma

Kang

Loomis

Yaspan³

et al. 2014

Eye

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“…NO donors were also shown to increase blood flow in the retina, choroid and the optic nerve head (12,13) . Additionally, some studies have addressed the beneficial effect of S-nitrosothiols on the reduction of intraocular pressure (14)(15)(16)(17) , and suggest that S-nitrosothiols are potential new drugs for the treatment of glaucoma and other ocular ischemic diseases (13) . Nitric oxide-mediated antimicrobial activity has also been the fo cus of recent research (18,19) .…”

Section: Introductionmentioning

confidence: 99%

Assessment of ocular surface toxicity after topical instillation of nitric oxide donors

Cariello¹,

Souza²,

Lowen³

et al. 2013

Arq. Bras. Oftalmol.

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Ethic committee from UNIFESP: number 1573/08. ABSTRACTPurpose: To evaluate the ocular surface toxicity of two nitric oxide donors in ex vivo and in vivo animal models: S-nitrosoglutathione (GSNO) and S-nitroso-Nace tylcysteine (SNAC) in a hydroxypropyl methylcellulose (HPMC) matrix at final concentrations 1.0 and 10.0 mM. Methods: Ex vivo GSNO and SNAC toxicities were clinically and histologically ana lyzed using freshly excised pig eyeballs. In vivo experiments were performed with 20 albino rabbits which were randomized into 4 groups (5 animals each): Groups 1 and 2 received instillations of 150 µL of aqueous HPMC solution containing GSNO 1.0 and 10.0 mM, respectively, in one of the eyes; Groups 3 and 4 received instillations of 150 µL of aqueous HPMC solution-containing SNAC 1.0 and 10.0 mM, respectively, in one of the eyes. The contralateral eyes in each group received aqueous HPMC as a control. All animals underwent clinical evaluation on a slit lamp and the eyes were scored according to a modified Draize eye test and were histologically analyzed. Results: Pig eyeballs showed no signs of perforation, erosion, corneal opacity or other gross damage. These findings were confirmed by histological analysis. The re was no difference between control and treated rabbit eyes according to the Draize eye test score in all groups (p>0.05). All formulations showed a mean score under 1 and were classified as "non-irritating". There was no evidence of tissue toxicity in the histological analysis in all animals. Conclusion: Aqueous HPMC solutions containing GSNO and SNAC at concentrations up to 10.0 mM do not induce ocular irritation.

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The effect of L-arginine on intraocular pressure in the human eye

Abstract: These findings demonstrated that L-arginine lowers IOP mainly through the formation of NO.

Cited by 34 publications

References 16 publications

Effect of Nitric Oxide on Anterior Segment Physiology in Monkeys

Effect of Nitric Oxide on Anterior Segment Physiology in Monkeys

Vascular tone pathway polymorphisms in relation to primary open-angle glaucoma

Assessment of ocular surface toxicity after topical instillation of nitric oxide donors

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