The effect of Johne's disease on production traits in Romney, Merino and Merino x Romney-cross ewes

Morris, C. A.; Hickey, S. M.; Hv, Henderson

doi:10.1080/00480169.2006.36698

Cited by 21 publications

(12 citation statements)

References 5 publications

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“…Johne's disease caused by Mycobacterium avium subspecies paratuberculosis (MAP), is an economically significant disease of ruminant species particularly cattle and sheep (Ott et al, 1999; Morris et al, 2006). MAP vaccines used in livestock contain heat-killed (Gudair® 316F strain, Mycopar strain 18, ID-Lelystad and 5889 Bergey) or live modified (Neoparasec-strain 316F and OSLO-strain 316F/2E) MAP cells Rosseels and Huygen (2008).…”

Section: Introductionmentioning

confidence: 99%

Cellular and humoral immune responses in sheep vaccinated with candidate antigens MAP2698c and MAP3567 from Mycobacterium avium subspecies paratuberculosis

Gurung

Purdie

Whittington

et al. 2014

Front. Cell. Infect. Microbiol.

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Control of Johne's disease, caused by Mycobacterium avium subspecies paratuberculosis (MAP) in ruminants using commercially available vaccine reduces production losses, mortality, fecal shedding and histopathological lesions but does not provide complete protection from infection and interferes with serological diagnosis of Johne's disease and bovine tuberculosis. At this time no recombinant antigens have been found to provide superior protection compared to whole killed or live-attenuated MAP vaccines. Therefore, there is a need to evaluate more candidate MAP antigens. In this study recombinant MAP antigens MAP2698c and MAP3567 were formulated with four different MONTANIDE™ (ISA 50V2, 61VG, 71VG, and 201VG) adjuvants and evaluated for their ability to produce specific immune responses in vaccinated sheep. The cellular immune response was measured with an interferon-gamma (IFN-γ) release assay and the humoral immune response was measured by antibody detection enzyme linked immunosorbent assay. Recombinant vaccine formulation with the antigen MAP2698c and MONTANIDE™ ISA 201VG adjuvant produced strong whole-MAP as well as MAP2698c-specific IFN-γ responses in a high proportion of the vaccinated sheep. The formulation caused less severe injection site lesions in comparison to other formulations. The findings from this study suggest that the MAP2698c + 201VG should be evaluated in a challenge trial to determine the efficacy of this vaccine candidate.

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Section: Introductionmentioning

confidence: 99%

Cellular and humoral immune responses in sheep vaccinated with candidate antigens MAP2698c and MAP3567 from Mycobacterium avium subspecies paratuberculosis

Gurung

Purdie

Whittington

et al. 2014

Front. Cell. Infect. Microbiol.

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“…The incidence of ovine paratuberculosis has been estimated to be 0.9 to 1.3% per annum among farmed sheep in New Zealand (20) and 5 to 15% per annum in Australia (24). It is difficult to accurately determine the true prevalence of Johne's disease due to the subclinical disease state (which can last for several years) and limitations in the available diagnostic techniques.…”

mentioning

confidence: 99%

Identification of Immune Parameters To Differentiate Disease States among Sheep Infected with Mycobacterium avium subsp. paratuberculosis

Gillan

O’Brien

Hughes

et al. 2010

Clin Vaccine Immunol

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Johne's disease, a chronic enteritis of ruminants, is caused by infection with Mycobacterium avium subsp. paratuberculosis. Three distinct forms have been observed in sheep: paucibacillary disease (PB), multibacillary disease (MB), and asymptomatic infection (AS). In this study, immune parameters for animals naturally infected with M. avium subsp. paratuberculosis and identified postmortem as having PB, MB, or AS were compared to provide a further understanding of the immunological reactivity contributing to or resulting from these different disease states in sheep. PB was associated with strong ex vivo M. avium subsp. paratuberculosis antigen-stimulated gamma interferon responses, pronounced increases in CD25 ؉ T-cell frequencies in circulation, antibody production, and a B-cell population that expanded significantly upon ex vivo antigenic stimulation. The MB group featured the highest antibody levels and a lack of cellular immune responsiveness to the M. avium subsp. paratuberculosis antigen. The AS group expressed an immunological phenotype intermediate between that for noninfected control animals and that for the PB group. The relationship between immune responses and disease severity within the PB group was investigated more closely; significant positive correlations were observed between disease severity and both the CD8 ؉ population in the circulating blood and the expression of interleukin-4 mRNA in antigen-stimulated blood samples ex vivo.

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“…paratuberculosis. Economic losses arise due to culling, reduced production of milk and wool, and mortalities (1,2). The disease is characterized by a long incubation period, and subclinical infection creates a potential source of infection for uninfected animals.…”

mentioning

confidence: 99%

Antigenicity of Recombinant Maltose Binding Protein-Mycobacterium avium subsp. paratuberculosis Fusion Proteins with and without Factor Xa Cleaving

Gurung

Begg

Purdie

et al. 2013

Clin Vaccine Immunol

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c Mycobacterium avium subsp. paratuberculosis causes Johne's disease (JD) in ruminants. Proteomic studies have shown that M. avium subsp. paratuberculosis expresses certain proteins when exposed to in vitro physiological stress conditions similar to the conditions experienced within a host during natural infection. Such proteins are hypothesized to be expressed in vivo, are recognized by the host immune system, and may be of potential use in the diagnosis of JD. In this study, 50 recombinant maltose binding protein (MBP)-M. avium subsp. paratuberculosis fusion proteins were evaluated using serum samples from sheep infected with M. avium subsp. paratuberculosis, and 29 (58%) were found to be antigenic. Among 50 fusion proteins, 10 were evaluated in MBP fusion and factor Xa-cleaved forms. A total of 31 proteins (62%) were found to be antigenic in either MBP fusion or factor Xa-cleaved forms. Antigenicity after cleavage and removal of the MBP tag was marginally enhanced.

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The effect of Johne's disease on production traits in Romney, Merino and Merino x Romney-cross ewes

Cited by 21 publications

References 5 publications

Cellular and humoral immune responses in sheep vaccinated with candidate antigens MAP2698c and MAP3567 from Mycobacterium avium subspecies paratuberculosis

Cellular and humoral immune responses in sheep vaccinated with candidate antigens MAP2698c and MAP3567 from Mycobacterium avium subspecies paratuberculosis

Identification of Immune Parameters To Differentiate Disease States among Sheep Infected with Mycobacterium avium subsp. paratuberculosis

Antigenicity of Recombinant Maltose Binding Protein-Mycobacterium avium subsp. paratuberculosis Fusion Proteins with and without Factor Xa Cleaving

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