The effect of jet speed on large volume jet injection

McKeage, James W.; Ruddy, Bryan P.; Nielsen, Poul M. F.; Taberner, Andrew J.

doi:10.1016/j.jconrel.2018.04.054

Cited by 45 publications

(14 citation statements)

References 22 publications

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“…pulsed lasers (850 m/s) and voice coils (100 m/s). 22,28,48,49 However, as it is evidenced in this study, thermocavitation jets seem to perfuse ex vivo porcine skin. Since this method is still in its infancy, no reliable studies have been performed on the potential physicochemical changes that the heating of the liquid might induce.…”

Section: Introductionmentioning

confidence: 54%

Delivery Strategies for Skin: Comparison of Nanoliter Jets, Needles and Topical Solutions

Bansal

Mitragotri

et al. 2019

Ann Biomed Eng

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Drug diffusion within the skin with a needle-free micro-jet injection (NFI) device was compared with two wellestablished delivery methods: topical application and solid needle injection. A permanent make-up (PMU) machine, normally used for dermal pigmentation, was utilized as a solid needle injection method. For NFIs a continuous wave (CW) laser diode was used to create a bubble inside a microfluidic device containing a light absorbing solution. Each method delivered two different solutions into ex vivo porcine skin. The first solution consisted of a red dye (direct red 81) and rhodamine B in water. The second solution was direct red 81 and rhodamine B in water and glycerol. We measured the diffusion depth, width and surface area of the solutions in all the injected skin samples. The NFI has a higher vertical dispersion velocity of 3 9 10 5 lm/s compared to topical (0.1 lm/s) and needle injection (53 lm/s). The limitations and advantages of each method are discussed, and we conclude that the micro-jet injector represents a fast and minimally invasive injection method, while the solid needle injector causes notable tissue damage. In contrast, the topical method had the slowest diffusion rate but causes no visible damage to the skin.

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Section: Introductionmentioning

confidence: 54%

Delivery Strategies for Skin: Comparison of Nanoliter Jets, Needles and Topical Solutions

Bansal

Mitragotri

et al. 2019

Ann Biomed Eng

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“…The fat only samples were obtained from pork belly purchased at a local butcher, with the skin and muscle layers carefully removed. All samples were stored in a −80°C freezer and thawed to room temperature (∼22°C) prior to experimentation, a handling procedure consistent with other jet injection studies .…”

Section: Methodsmentioning

confidence: 99%

Spatially resolved diffuse imaging for high‐speed depth estimation of jet injection

Brennan

Ruddy

Nielsen

et al. 2019

Journal of Biophotonics

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We investigate the use of spatially resolved diffuse imaging to track a fluid jet delivered at high speed into skin tissue. A jet injector with a short needle to deliver drugs beneath the dermis, is modified to incorporate a laser beam into the jet, which is ejected into ex vivo porcine tissue. The diffuse light emitted from the side and top of the tissue sample is recorded using high‐speed videography. Similar experiments, using a depth‐controlled fiber optic source, generate a reference dataset. The side light distribution is related to source depth for the controlled‐source experiments and used to track the effective source depth of the injections. Postinjection X‐ray images show agreement between the jet penetration and ultimate light source depth. The surface light intensity profile is parameterized with a single parameter and an exponential function is used to relate this parameter to source depth for the controlled‐source data. This empirical model is then used to estimate the effective source depth from the surface profile of the injection experiments. The depth estimates for injections into fat remain close to the side depth estimates, with a root‐mean‐square error of 1.1 mm, up to a source depth of 8 mm.

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“…9,10 In this study, a naked plasmid was injected using pig abdominal skin, which is most commonly used in jet injection studies. [11][12][13][14] We attempted to use the pyro-drive jet injector capable of controlling injection depth and volume via a biphasic injection profile 15 to significantly enhance gene expression in ID delivery. The depth of the tissue injection can be controlled by modulating the amount of ignition powder (IP) generating the first pressure peak, and the liquid volume injected into the tissue increased with the use of smokeless powder (SP), generating a second pressure peak.…”

Section: Introductionmentioning

confidence: 99%

Potent Intradermal Gene Expression of Naked Plasmid DNA in Pig Skin Following Pyro-drive Jet Injection

Miyazaki

Sakaguchi

Terai

2021

Journal of Pharmaceutical Sciences

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Intradermal administration of naked DNA with a conventional needle syringe is a simple and inexpensive method to expose an encoded antigen to the dermal immune system. We aimed to enhance intradermal gene expression with a pyro-drive jet injector using pig skin, which is similar in structure and biomechanical properties to human skin. When Cy3-labeled plasmid (pCy3) was applied to pig skin with the jet injector, pCy3 was distributed preferentially in the intradermal tissue. Precise localization analysis revealed that pCy3 was also detected in the intracellular nucleus, and the frequency was substantially higher with the jet injector than with a needle syringe. When a luciferase expression plasmid (pLuc) was injected transdermally, the luciferase activity was 380-fold higher with the jet injector than with a needle syringe. Furthermore, immunohistochemistry analysis showed that the epidermis was positive for luciferase protein expression. These data indicate that the jet injector facilitates stable intradermal administration, resulting in more efficient gene expression compared to that with conventional syringe methods. Thus, intradermal administration of an antigen-expression plasmid with the pyro-drive jet injector may provide a clinically viable method for future gene therapy.

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The effect of jet speed on large volume jet injection

Cited by 45 publications

References 22 publications

Delivery Strategies for Skin: Comparison of Nanoliter Jets, Needles and Topical Solutions

Delivery Strategies for Skin: Comparison of Nanoliter Jets, Needles and Topical Solutions

Spatially resolved diffuse imaging for high‐speed depth estimation of jet injection

Potent Intradermal Gene Expression of Naked Plasmid DNA in Pig Skin Following Pyro-drive Jet Injection

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