The effect of Java Plum Fruit (Zyzygium cumini) extract on leucocyte and lung histopathology of mouse exposed cigarette smoke

Abstract

Java Plum fruit can decrease free radical activities. The overwhelmed production of free radicals will reduce the immunity system in the body. This research aims to study the effects of java plum fruit extracts on leukocytes and histopathology of mouse lungs that have been exposed to cigarette smoke for 30 days. Frozen-dried java plum fruit is macerated with water. The mice are divided into Control groups (K-). The mice of the control group were given only water orally. (K+) are mice that are exposed to commercial cigarette smoke once a day. (RJ) were the mice exposed to cigarette smoke once a day as java plum fruit extract orally of 180 mg/kg body weight. (RF) were the mice exposed to cigarette smoke given with java plum fruit extract with 180 mg/kg of body weight that is applied on its filter. The results showed a significantly lower number of leukocytes (p < 0.05) in the K-,RF, and RJ groups compared to K+. Meanwhile, there was a significant increase in the number of lung pathological cells (p < 0.05) in the K+, RF, and RJ compared to the control group (K-). The results conclude that java plum fruit can reduce free radicals in animals affected by inflammation due to cigarette smoke particles in the lungs.