The effect of isotretinoin on propylene glycol-induced cholesteatoma in chinchilla middle ears

Jove, Margaret A.; Vassalli, Luca; Raslan, Wasim F.; Applebaum, Edward L.

doi:10.1016/0196-0709(90)90163-p

Cited by 11 publications

(16 citation statements)

References 8 publications

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“…This higher rate of cholesteatoma production was probably due to the use of latex polymerized with 50% propylene glycol, since latex induces a greater inflammatory reaction and angiogenesis, favoring epithelium migration from the external auditory meatus. Propylene glycol acts as a chemical irritant which, when in contact with the tympanic membrane, may cause a break of the lamina propria of the latter and cause migration or invagination of squamous epithelium 7 …”

Section: Discussionmentioning

confidence: 99%

“…In all experiments in which irritating substances were used, such as propylene glycol, 7 ticarcillin, 8 outer auditory meatus skin, 9 and modified collagen, 6 the inflammatory reaction was of the utmost importance for the induction of cholesteatomas, since, according to the authors of the cited reports, it functioned as a factor inducing epithelial migration or invagination in the outer auditory meatus or in the tympanic membrane toward the middle ear.…”

Section: Introductionmentioning

confidence: 99%

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A New Experimental Model of Acquired Cholesteatoma

Massuda

Oliveira

2005

The Laryngoscope

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The presence of inflammatory cells may provoke the production of cytokines (IL-1, IL-2, IL-6, IL-8) and growth factors, which, together with the latex biomembrane, which is known to contain a protein that favors vascular growth, may cause chemotactic migration of the squamous epithelium from the external auditory meatus to the middle year of the rat, causing a cholesteatoma. The induction of an experimental cholesteatoma in rats with latex and latex plus 50% propylene glycol was effective, representing an excellent experimental model. The theory of epithelial migration in the genesis of cholesteatomas was confirmed by our observations in rats. The latex induced an acute and chronic inflammatory reaction when in contact with the mucosa of the middle ear.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A New Experimental Model of Acquired Cholesteatoma

Massuda

Oliveira

2005

The Laryngoscope

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“…8,9 There are a number of possible explanations for these findings, such as the use of a vitamin A analogue. One possibility is that isotretinoin treatment was done prior to the use of an irritating agent; findings might have been different if both agents had been used simultaneously.…”

Section: Discussionmentioning

confidence: 99%

“…Isotretinoin, a synthetic retinol analogue, was used systemically with poor cholesteatoma-inhibiting results. 9 All-trans retinoic acid is used topically to treat acne vulgaris. It decreases epithelial cell adhesion and is effective in skin keratinization disorders; it also helps repair skin following exposure to phototherapy or other external agents.…”

Section: Introductionmentioning

confidence: 99%

Efeito do ácido trans-retinóico na inibição de colesteatoma em cobaias

Antunes

Fukuda

Penido

et al. 2008

Rev. Bras. Otorrinolaringol.

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Middle ear cholesteatoma affected more than 5 million people until the 80`s. Many animal models were used, unsuccessfully, to study an alternative therapy to cholesteatoma. Aim: observe the effect of the trans-retinoic acid in the inhibition of middle ear cholesteatomas induced by propylene glycol. Study Design: Clinical and Experimental. Methods: 25 guinea pigs were submitted to the application of a 100% propylene glycol solution in their bulla bilaterally and a solution of trans-retinoic acid was applied locally in the external right ear, while in the left ear saline solution was applied (control ear). The guinea pigs were slaughtered and their temporal bones were prepared for macroscopic and histological analysis. Results: The macroscopic findings had evidenced the presence of cholesteatoma in 25% of the right ears and 85% of the left ears (P=0.0003 *). The histological study had evidenced the presence of cholesteatoma in 30% of right ears and 75% of the left ears (P=0.0104*). Conclusion: The local use of the trans-retinoic acid is effective in inhibiting the induced formation of cholesteatomas in guinea pigs.

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“…The Mongolian gerbil develops spontaneous cholesteatoma (47)(48)(49), which is known to cause bone resorption and is used as a cholesteatoma model. Additionally, chinchillas and guinea pigs have been used as cholesteatoma models (50,51). Recently, it was also reported that overexpression of KGF by electroporation of KGF cDNA leads to cholesteatoma formation in rats (52).…”

Section: Discussionmentioning

confidence: 99%

Intercellular Communication between Keratinocytes and Fibroblasts Induces Local Osteoclast Differentiation: a Mechanism Underlying Cholesteatoma-Induced Bone Destruction

Iwamoto

Nishikawa

Imai

et al. 2016

Molecular and Cellular Biology

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Bone homeostasis is maintained by a balance in activity between bone-resorbing osteoclasts and bone-forming osteoblasts. Shifting the balance toward bone resorption causes osteolytic bone diseases such as rheumatoid arthritis and periodontitis. Osteoclast differentiation is regulated by receptor activator of nuclear factor B ligand (RANKL), which, under some pathological conditions, is produced by T and B lymphocytes and synoviocytes. However, the mechanism underlying bone destruction in other diseases is little understood. Bone destruction caused by cholesteatoma, an epidermal cyst in the middle ear resulting from hyperproliferation of keratinizing squamous epithelium, can lead to lethal complications. In this study, we succeeded in generating a model for cholesteatoma, epidermal cyst-like tissue, which has the potential for inducing osteoclastogenesis in mice. Furthermore, an in vitro coculture system composed of keratinocytes, fibroblasts, and osteoclast precursors was used to demonstrate that keratinocytes stimulate osteoclast differentiation through the induction of RANKL in fibroblasts. Thus, this study demonstrates that intercellular communication between keratinocytes and fibroblasts is involved in the differentiation and function of osteoclasts, which may provide the molecular basis of a new therapeutic strategy for cholesteatoma-induced bone destruction. Bone is a highly dynamic organ in which homeostasis is maintained by a balance in activity between bone-resorbing osteoclasts and bone-forming osteoblasts. An imbalance between osteoclastic and osteoblastic activity causes various skeletal disorders. Osteoclasts are multinucleate cells that differentiate from mononuclear macrophage/monocyte-lineage hematopoietic precursor cells, which are attracted to the bloodstream by factors, including sphingsine-1 phosphate (1, 2). Osteoclast differentiation and function are regulated by macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor B ligand (RANKL) (3-5). Under physiological conditions, osteoblasts or osteocytes are the source of RANKL (6-8). RANKL-mediated osteoclastogenesis also plays an important role in bone destruction in inflammatory bone diseases such as rheumatoid arthritis (RA) or periodontal diseases. In such pathological states, synoviocytes or immune cells, such as B or T lymphocytes, infiltrating into the area are proposed to be the major sources of RANKL (9-11). In RA, infiltration of T helper 17 (Th17) cells is observed in the synovium. Th17 cells indirectly induce RANKL expression in synoviocytes by producing interleukin-17 (IL-17) (12), and Th17 cells themselves express RANKL on their plasma membrane (13). Thus, in many bone destructive diseases, the mechanisms of bone destruction are becoming clarified in detail. However, there are still certain bone destructive diseases whose underlying mechanisms remain to be elucidated. Cholesteatoma, a benign entity arising in temporal bone, is one such disease where our understanding is limited.Cholesteatoma is an epid...

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The effect of isotretinoin on propylene glycol-induced cholesteatoma in chinchilla middle ears

Cited by 11 publications

References 8 publications

A New Experimental Model of Acquired Cholesteatoma

A New Experimental Model of Acquired Cholesteatoma

Efeito do ácido trans-retinóico na inibição de colesteatoma em cobaias

Intercellular Communication between Keratinocytes and Fibroblasts Induces Local Osteoclast Differentiation: a Mechanism Underlying Cholesteatoma-Induced Bone Destruction

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