The effect of isosteviol on hyperglycemia and dyslipidemia induced by lipotoxicity in rats fed with high-fat emulsion

Xu, Duo; Xu, Min; Lin, Lin; Rao, Shasha; Wang, Jiping; Davey, Andrew K.

doi:10.1016/j.lfs.2011.10.010

Cited by 27 publications

(16 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this work, the hyperlipidemia was well induced by high-fat emulsion, and this induction was utilized in animal models (Zhao, Huang, & Yuan, 2012;Xu, Xu, Lin, Rao, Wang, & Davey, 2012;Irudayaraj, Sunil, Duraipandiyan, & Ignacimuthu, 2013).…”

Section: Introductionmentioning

confidence: 99%

The antihyperlipidemic activities of enzymatic and acidic intracellular polysaccharides by Termitomyces albuminosus

Zhao

Zhang

et al. 2016

Carbohydrate Polymers

Self Cite

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

The antihyperlipidemic activities of enzymatic and acidic intracellular polysaccharides by Termitomyces albuminosus

Zhao

Zhang

et al. 2016

Carbohydrate Polymers

Self Cite

View full text Add to dashboard Cite

“…Isosteviol, a diterpene glycoside isolated from the leaves of Stevia rebaudiana , has been reported to have anti-diabetic properties in rodents and humans [32-34]. It appears to increase insulin sensitivity, making it a candidate supplement in insulin resistant states for preventing type 2 diabetes.…”

Section: Resultsmentioning

confidence: 99%

Development and preliminary validation of a plate-based CB1/CB2 receptor functional assay

Dossou

Devkota

Kavanagh

et al. 2013

Analytical Biochemistry

View full text Add to dashboard Cite

Cannabinoids receptors (CB) are being targeted therapeutically for the treatment of anxiety, obesity, movement disorders, glaucoma, and pain. More recently, cannabinoid agonists have displayed anti-proliferative activity against breast cancer and prostate cancer in animal models. In order to study cannabinoid receptor ligands, we have developed a novel plate-based assay that measures internalization of CB1/CB2 receptors by determining the change in the intracellular levels of the radiolabeled agonists: [3H]-Win 55-212-2 for CB1 and [3H]-CP 55-940 for CB2. The developed plate-based assay was validated by determining IC50 values for known antagonists: AM251, AM281, AM630 and AM6545. The data obtained was consistent with previously reported values, therefore confirming that the assay can be used to determine the functional binding activities (IC50) of antagonists for the CB1 and CB2 receptors. In addition, we demonstrated that the plate-based assay may be used for screening against complex matrices. Specifically, we demonstrated that the plate-based assay was able to identify which extracts of several species of the genus Zanthoxylum had activity at the CB1/CB2 receptors.

show abstract

“…In the first set of in vivo experiments, the four groups were ND, HFD, HFD+ISV (10 mg/kg), and HFD+ISV (20 mg/kg). Our previous study showed that 5 mg/kg ISV had only marginal effect on high-fat emulsion-induced TG increase (51).…”

Section: Animalsmentioning

confidence: 91%

“…It has been reported that ISV has protective effects against HFD-induced hyperglycemia and dyslipidemia (51). To determine whether ISV has any effect on HFD-induced hepatic steatosis and dysregulation of lipid metabolism, HFD-fed rat NAFLD models were used.…”

Section: Isv Inhibited Hfd-induced Hepatic Injury In Rat Nafld Modelsmentioning

confidence: 99%

“…ISV is an acid hydrolysate of stevioside, the major component of Stevia rebaudiana, and is used as a sweetener. Stevioside and its metabolites have been shown to have varieties of pharmacological activities such as antidiabetic and antihyperlipidemia effects (51). However, stevioside not only has toxic and mutagenic effects but also has very low oral bioavailability (31).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Isosteviol Protects Free Fatty Acid- and High Fat Diet-Induced Hepatic InjuryviaModulating PKC-β/p66Shc/ROS and Endoplasmic Reticulum Stress Pathways

et al. 2019

Antioxidants & Redox Signaling

Self Cite

View full text Add to dashboard Cite

Aims: Nonalcoholic fatty liver disease (NAFLD) is the most common cause of chronic liver diseases. However, there are no approved pharmacotherapies for the treatment of NAFLD other than managing life style and controlling diets. Extensive studies have demonstrated that multiple mechanisms are involved in free fatty acid (FFA)-and high fat diet (HFD)-induced hepatic injury, including mitochondrial dysfunction, activation of oxidative stress and endoplasmic reticulum (ER) stress, and lysosome dysfunction. A previous study reported that Isosteviol (ISV), a derivative of stevioside, prevents HFD-induced hepatic injury. However, the underlying mechanisms remain unclear. Results: In this study, we examined the potential cellular/molecular mechanisms underlying ISV-mediated protective effect against FFA-/HFD-induced hepatic lipotoxicity by using both in vitro primary rat hepatocytes and the in vivo rat NAFLD model. The results indicated that ISV inhibits FFA-/HFD-induced hepatic injury via reducing oxidative and ER stress. Specifically, ISV inhibited the expression, activation, and mitochondrial translocation of Src-homology-2-domain-containing transforming protein 1 (p66Shc), an adapter protein that mediates oxidative stress-induced injury and is a substrate of protein kinase C-b (PKC-b), via inhibition of PKC-b activity. However, ISV had no effect on the expression and activity of peptidyl-prolyl cis-trans isomerase and serine/threonine protein phosphatase 2A, isomerase and phosphorylase of p66Shc. In addition, ISV also inhibited FFA-induced ER stress and decreased ER-mitochondrial interaction. Innovation and Conclusion: We first identified that ISV prevents FFA-/HFD-induced hepatic injury through modulating PKC-b/p66Shc/oxidative and ER stress pathways. ISV represents a promising therapeutic agent for NAFLD in the future.

show abstract

The effect of isosteviol on hyperglycemia and dyslipidemia induced by lipotoxicity in rats fed with high-fat emulsion

Cited by 27 publications

References 45 publications

The antihyperlipidemic activities of enzymatic and acidic intracellular polysaccharides by Termitomyces albuminosus

The antihyperlipidemic activities of enzymatic and acidic intracellular polysaccharides by Termitomyces albuminosus

Development and preliminary validation of a plate-based CB1/CB2 receptor functional assay

Isosteviol Protects Free Fatty Acid- and High Fat Diet-Induced Hepatic InjuryviaModulating PKC-β/p66Shc/ROS and Endoplasmic Reticulum Stress Pathways

Contact Info

Product

Resources

About