The Effect of Isoflurane, Halothane and Pentobarbital on Noise-Induced Hearing Loss in Mice

Chung, Jong Woo; Ahn, Joong Ho; Kim, Jong Yang; Lee, Hyun Jung; Kang, Hun Hee; Lee, Yoon Kyung; Kim, Joung Uk; Koo, Seung-Woo

doi:10.1213/01.ane.0000261508.24083.6c

Cited by 33 publications

(26 citation statements)

References 20 publications

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“…Isoflurane could thereby provide a neuroprotective effect, preventing damage to hair cells (as shown by Kim et al, 2005) or central auditory neurons. Since the neuroprotective effect of isoflurane reduces noise-induced hearing loss (Kim et al, 2005;Chung et al, 2007), this mechanism could directly explain the prevention of temporary tinnitus that we observed. Although we demonstrated that hearing loss at 6 kHz is unlikely to account for the gap-detection deficits that we used to measure tinnitus, it is still likely that the temporary tinnitus was caused by noise-induced hearing loss at higher frequencies.…”

Section: Discussionmentioning

confidence: 68%

“…For experimental design of tinnitus studies, the use of anesthesia during noise exposure must be taken into careful consideration. While we used isoflurane because of the need for rapid recovery in our paradigm, other anesthetics such as pentobarbital have also been shown to reduce noise-induced hearing loss in mice (Chung et al, 2007) and might therefore show preventative effects on tinnitus similar to isoflurane. However, it is probably not necessary to conclude that anesthesia should never be used during noise exposure in tinnitus studies.…”

Section: Discussionmentioning

confidence: 99%

“…Because the animal is only anesthetized for 3e4 min, we can measure gap detection immediately before and directly after acoustic trauma. However, isoflurane has recently been reported to have a neuroprotective effect on noise-induced hearing loss in mice (Kim et al, 2005;Chung et al, 2007). This could block temporary tinnitus by preventing noise-induced hearing loss.…”

Section: Introductionmentioning

confidence: 99%

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Isoflurane blocks temporary tinnitus

2012

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Isoflurane blocks temporary tinnitus

2012

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“…Animals were exposed to 106-dB octave-band (8–16 kHz) white noise for 60 minutes in a custom-built, calibrated, reverberant sound chamber. Animals were unanesthetized in order to avoid affects of general anesthesia on sound-induced threshold shifts [27]. Click ABR thresholds were measured again at 1, 3, 7, and 18 days after noise exposure.…”

Section: Methodsmentioning

confidence: 99%

Sound-Induced Intracellular Ca2+ Dynamics in the Adult Hearing Cochlea

Chan

Rouse

2016

PLoS ONE

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Ca2+ signaling has been implicated in the initial pathophysiologic mechanisms underlying the cochlea's response to acoustic overstimulation. Intracellular Ca2+ signaling (ICS) waves, which occur in glia and retinal cells in response to injury to activate cell regulatory pathways, have been proposed as an early event in cochlear injury. Disruption of ICS activity is thought to underlie Connexin 26-associated hearing loss, the most common genetic form of deafness, and downstream sequelae of ICS wave activity, such as MAP kinase pathway activation, have been implicated in noise-induced hearing loss. However, ICS waves have only been observed in neonatal cochlear cultures and are thought to be quiescent after the onset of hearing. In this study, we employ an acute explant model of an adult, hearing cochlea that retains many in vivo physiologic features to investigate Ca2+ changes in response to sound. We find that both slow monotonic changes in intracellular Ca2+ concentration as well as discrete ICS waves occur with acoustic overstimulation. The ICS waves share many intrinsic features with their better-described neonatal counterparts, including ATP and gap-junction dependence, and propagation velocity and distance. This identification of ICS wave activity in the adult, hearing cochlea thus confirms and characterizes an important early detection mechanism for cochlear trauma and provides a target for interventions for noise-induced and Connexin 26-associated hearing loss.

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“…However, isoflurane has been shown to diminish the amplitude and duration of temporary tinnitus after a short exposure to loud sounds [25]. Under pentobarbital, isoflurane, or halothane anesthesia noise-induced hearing loss in mice is less (62.5 dB, 45.5 dB, 39.3 dB threshold increase, respectively) compared to the unanesthetized control group (77.5 dB threshold increase) [115]. In addition, the influence of anesthesia on any electrophysiological recordings has to be taken into account, as anesthesia influences the receptive fields and the spontaneous activity of the rat auditory cortex [116].…”

Section: Established Ways Of Tinnitus Induction In Animal Researchmentioning

confidence: 99%

Animal Models of Subjective Tinnitus

Behrens

2014

Neural Plasticity

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Tinnitus is one of the major audiological diseases, affecting a significant portion of the ageing society. Despite its huge personal and presumed economic impact there are only limited therapeutic options available. The reason for this deficiency lies in the very nature of the disease as it is deeply connected to elementary plasticity of auditory processing in the central nervous system. Understanding these mechanisms is essential for developing a therapy that reverses the plastic changes underlying the pathogenesis of tinnitus. This requires experiments that address individual neurons and small networks, something usually not feasible in human patients. However, in animals such invasive experiments on the level of single neurons with high spatial and temporal resolution are possible. Therefore, animal models are a very critical element in the combined efforts for engineering new therapies. This review provides an overview over the most important features of animal models of tinnitus: which laboratory species are suitable, how to induce tinnitus, and how to characterize the perceived tinnitus by behavioral means. In particular, these aspects of tinnitus animal models are discussed in the light of transferability to the human patients.

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The Effect of Isoflurane, Halothane and Pentobarbital on Noise-Induced Hearing Loss in Mice

Abstract: These findings indicate that, while halothane, isoflurane and pentobarbital could protect mice against NIHL and hair cell damage, inhaled anesthetics were more effective.

Cited by 33 publications

References 20 publications

Isoflurane blocks temporary tinnitus

Isoflurane blocks temporary tinnitus

Sound-Induced Intracellular Ca2+ Dynamics in the Adult Hearing Cochlea

Animal Models of Subjective Tinnitus

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