The effect of ischemic time and temperature on lung preservation in a simple ex vivo rabbit model used for functional assessment

Wang, Liang-Shun; Yoshikawa, Kunio; Miyoshi, Shinichiro; Nakamoto, Keiichi; Hsieh, Chia-Ming; Yamazaki, Fumio; Cardoso, Paulo Francisco Guerreiro; G, Hans-Joachim Schaefers; Brito, Julio; Keshavjee, Shaf; Patterson, Alexander; Cooper, Joel D.

doi:10.1016/s0022-5223(19)34378-8

Cited by 90 publications

(18 citation statements)

References 14 publications

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“…Machine preservation of solid organs is being proposed as a method that may achieve these goals (10)(11)(12)(13)(14). (15)(16)(17)(18)(19). It also has been shown to be a useful technique to briefly (1 to 2 h) evaluate lungs from donation after cardiac death (DCD) (20)(21)(22).…”

Section: Introductionmentioning

confidence: 99%

Normothermic Ex Vivo Perfusion Prevents Lung Injury Compared to Extended Cold Preservation for Transplantation

Cypel

Rubacha

Yeung

et al. 2009

American Journal of Transplantation

225

164

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Treatment of injured donor lungs ex vivo to accelerate organ recovery and ameliorate reperfusion injury could have a major impact in lung transplantation.We have recently demonstrated a feasible technique for prolonged (12 h) normothermic ex vivo lung perfusion (EVLP). This study was performed to examine the impact of prolonged EVLP on ischemic injury. Pig donor lungs were cold preserved in Perfadex R for 12 h and subsequently divided into two groups: cold static preservation (CSP) or EVLP at 37• C with Steen TM solution for a further 12 h (total 24 h preservation). Lungs were then transplanted and reperfused for 4 h. EVLP preservation resulted in significantly better lung oxygenation (PaO 2 531 ± 43 vs. 244 ± 49 mmHg, p < 0.01) and lower edema formation rates after transplantation. Alveolar epithelial cell tight junction integrity, evaluated by zona occludens-1 protein staining, was disrupted in the cell membranes after prolonged CSP but not after EVLP. The maintenance of integrity of barrier function during EVLP translates into significant attenuation of reperfusion injury and improved graft performance after transplantation. Integrity of functional metabolic pathways during normothermic perfusion was confirmed by effective gene transfer and GFP protein synthesis by lung alveolar cells. In conclusion, EVLP prevents ongoing injury associated with prolonged ischemia and accelerates lung recovery.

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Section: Introductionmentioning

confidence: 99%

Normothermic Ex Vivo Perfusion Prevents Lung Injury Compared to Extended Cold Preservation for Transplantation

Cypel

Rubacha

Yeung

et al. 2009

American Journal of Transplantation

225

164

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“…Optimal temperature for storage appears to be between 4 and 8 °C. Cold storage has been suggested to compound certain aspects of ischaemia-reperfusion injury, through increased pulmonary vasoconstriction and thus increased vascular resistance after reperfusion [121].…”

Section: Cold Storage Methodsmentioning

confidence: 99%

Review 1: Lung transplant—from donor selection to graft preparation

et al. 2020

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For various end-stage lung diseases, lung transplantation remains one of the only viable treatment options. While the demand for lung transplantation has steadily risen over the last few decades, the availability of donor grafts is limited, which have resulted in progressively longer waiting lists. In the early years of lung transplantation, only the 'ideal' donor grafts are considered for transplantation. Due to the donor shortages, there is ongoing discussion about the safe use of 'suboptimal' grafts to expand the donor pool. In this review, we will discuss the considerations around donor selection, donor-recipient matching, graft preparation and graft optimisation.

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“…In vivo and in vitro laboratory studies looking for the ideal temperature for lung preservation have determined the ideal preservation temperature to be 1 0°C. [23][24][25] The optimal volume of flush solution is unknown. A single dose of antegrade flushing is standard.…”

Section: Storage Solutionmentioning

confidence: 99%

Overview and Future Practice Patterns in Cardiac and Pulmonary Preservation

Conte

Baumgartner

2000

J Cardiac Surgery

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Heart and lung transplantation have become standard therapy for many patients with end-stage heart and lung disease. Successful transplantation requires preservation of allografts until they can be implanted and reperfused. In the decades since the transplantation of thoracic organs became a clinical reality, many advances have been made in preoperative donor management, procurement, and preservation techniques. This article summarizes the state of the art in heart and lung preservation and review some of the areas of current research that may lead to improvements in preservation techniques in the future.

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The effect of ischemic time and temperature on lung preservation in a simple ex vivo rabbit model used for functional assessment

Cited by 90 publications

References 14 publications

Normothermic Ex Vivo Perfusion Prevents Lung Injury Compared to Extended Cold Preservation for Transplantation

Normothermic Ex Vivo Perfusion Prevents Lung Injury Compared to Extended Cold Preservation for Transplantation

Review 1: Lung transplant—from donor selection to graft preparation

Overview and Future Practice Patterns in Cardiac and Pulmonary Preservation

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