The Effect of Ischemic Preconditioning Prior to Intraoperative Radiotherapy on Ischemic and on Reperfused Rat Liver

Hahn, Oszkár; Szíjártó, Attila; Lotz, Gábor; Schaff, Zsuzsa; Vigváry, Z; Váli, László; Kempler, Péter

doi:10.1016/j.jss.2006.10.026

Cited by 7 publications

(10 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Ischemic preconditioning could improve the physiological functions of internal organs by reducing the inflammatory responses in multiple tissues undergoing ischemia/reperfusion, which is an important manifestation of the endogenous protective mechanism of ischemic preconditioning. During radiation injury, tumor necrosis factor-α (a pro-inflammatory cytokine) plays an important role in escalating the inflammatory response, as evidenced by the significant increase in tumor necrosis factor-α levels in multiple tissues undergoing ischemia/ reperfusion and radiation [10,31,32]. In the present study, we observed markedly activated levels of inflammatory cell infiltration by detecting myeloperoxidase activity.…”

Section: Discussionsupporting

confidence: 57%

“…Research on animal models and clinical studies have shown that ischemic preconditioning has a powerful protective effect against ischemia/reperfusion injury in various organs, including the heart, intestine, lung, kidney, and skeletal muscle [4][5][6][7][8][9]. Studies have shown that ischemic preconditioning can protect the liver from the combined damage caused by ischemia/reperfusion and radiation with doses of up to 50 Gy [10]. Local temporary ischemic preconditioning can prevent the oxidative effects of fractionated radiotherapy in the small intestine and in the serum of rats following abdominal irradiation [11].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effect of ischemic preconditioning on radiation damage to the submandibular gland in rats

Yao

Liu

Song

et al. 2021

European J Oral Sciences

View full text Add to dashboard Cite

To investigate the effects of radiation on rat submandibular glands and the possible protective effects of ischemic preconditioning, the submandibular glands of Wistar rats were subjected to in situ radiation after ischemic preconditioning. The glands were exposed to X-radiation at a single dose of 20 Gy. Ischemic preconditioning was achieved by three min of ischemia and three min of reperfusion, repeated three times before irradiation. Salivary secretion, histological changes, alterations in tight junctions, and the levels of oxidative stress, pro-inflammatory cytokines, and water secretion proteins mediated by the muscarinic acetylcholine M3 subtype receptor were determined at 1 and 12 weeks post-irradiation. In glands subjected to irradiation only, the secretion, superoxide dismutase activity, tight junction width, acinar cell number, and M3 receptor and aquaporin-5 levels were lower at 1 and 12 weeks than seen in the ischemically preconditioned irradiated glands. In contrast, tumor necrosis factor-α, malondialdehyde, myeloperoxidase activity, and the expression of the tight junction protein claudin-4 were significantly higher in the irradiated only glands. Our study revealed that radiation caused a series of injury-stress responses, especially damage to the water secretion pathway mediated by the M3 receptor that ultimately led to hyposecretion, which might play an important role in the dysfunction of the irradiated only glands. Ischemic preconditioning reduced the radiation-induced injury to submandibular glands and ameliorated salivary hyposecretion.

show abstract

Section: Discussionsupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Effect of ischemic preconditioning on radiation damage to the submandibular gland in rats

Yao

Liu

Song

et al. 2021

European J Oral Sciences

View full text Add to dashboard Cite

show abstract

“…Notably, the myogenic differentiation and particularly priming of mesenchymal cells to cardiomyogenic differentiation takes place over a short period of time. Hahn et al (2007) submitted male Wistar rats into liver IR with or without ischemic precondition (IP). During IR, intraoperative radiotherapy (IORT) doses of 0-50 Gy were applied to the ischemic liver lobe.…”

Section: Discussionmentioning

confidence: 99%

The effect of the antioxidant drug “U-74389G” on alkaline phosphatase levels during ischemia reperfusion injury in rats

TSOMPOS¹,

PANOULİS²,

TOUTOUZAS³

et al. 2014

jecm

View full text Add to dashboard Cite

Article HistoryReceived 16 / 03 / 2014 Accepted 10 / 05 / 2014The aim of this experimental study was to examine the effect of the antioxidant drug "U-74389G", on rat model and particularly in a liver ischemia reperfusion (IR) protocol. The beneficial effect or non-effectiveness of that molecule were studied biochemically using mean blood alkaline phosphatase levels (ALP). Forty rats of mean weight 231.875 gr were used in the study. ALP levels were measured 60 min after reperfusion (groups A and C) and 120 min after reperfusion (groups B and D) with administration of the drug U-74389G in groups C and D. U-74389G administration non-significantly increased the ALP levels by 19.76%±15.06% (p=0.1784), but by 31.91%±7.69% (p=0.0001) for predicted values. Reperfusion time non-significantly decreased the ALP levels by 25.21%±14.83% (p=0.0675), also by 6.38%±9.30% (p=0.3650) for predicted values. However, U-74389G administration and reperfusion time together non-significantly increased the ALP levels by 8.08%±9.19% (p=0.3696), but also by 17.75%±4.79% (p=0.0005) for predicted values. This study indicates that U-74389G administration and its interaction with reperfusion time non-significantly increased short-term effects on ALP levels. However, the predicted ALP values adjusted for rats weight, kept significantly increased ones, which stand for the restorating effects of U-74389G, that are slower than it seems.

show abstract

“…A Murry és mtsai [20] által 1986-ban kardiológiai állat-kísérlet keretében felfedezett, prekondicionálás mérséklő szerepét munkacsoportunk szintén vizsgálta kísérletesen létrehozott, eltérő időtartamú parciális patkánymáj I-R [21], valamint parciális máj I-R mellett párhuzamosan elvégzett intraoperatív radioterápia kapcsán [22]. Eredményeink szerint a prekondicionálás mindkét esetben képes volt mérsékelni a máj I-R károsodását.…”

Section: Kondicionáló Eljárások éS Máj Ischaemiareperfúzió -áLlatkíséunclassified

“…Eredményeink szerint a prekondicionálás mindkét esetben képes volt mérsékelni a máj I-R károsodását. Ki emelendő a mindkét kísérlet során megfi gyelt redox-homeosztázis megtartottsága (kemiluminometria, tiolcsoportok, H + -donorok, redukálóerő), amelyhez feltehetőleg tevéke-nyen hozzájárult a prekondicionálás első kísérletben igazolt mikrocirkulációt javító hatása, valamint a második kísérletben megfi gyelt, radioterápia okozta sérülés mér-séklő hatása [21,22]. Przyklenk és munkatársainak [23] 1993-as, valamint Schmidt és munkatársainak [24] 2007-es, úttörő felfedezéseit követően a perkondicioná-ló kezelés tanulmányozásával laborunk is behatóan foglalkozott, mind annak háttérmechanizmusai [25], mind kísérletes vizsgálata kapcsán.…”

Section: Kondicionáló Eljárások éS Máj Ischaemiareperfúzió -áLlatkíséunclassified

Szabad gyökök és a máj ischaemiás-reperfúziós károsodása

Szíjártó

2015

Orvosi Hetilap

Self Cite

View full text Add to dashboard Cite

Az ischaemiás-reperfúziós károsodás kiemelkedő jelentősége számos szerv és klinikai szituáció esetén jól bizonyított. A posztoperatív komplikációk prevalenciáját, a célszerv sérülését és a szisztémás szövődmények előfordulását jelentő-sen meghatározó károsodás mediálásában központi szerep jut a szabad gyököknek. A szabadgyök-stresszre kimondottan érzékeny máj anatómiailag és patofi ziológiailag speciális ischaemiás-reperfúziós károsodása májsebészeti szempontból kulcsfontosságú. A reperfúziós károsodás mérséklésére számos kondicionáló eljárás (adaptív technikák, kémiai vegyületek) ismert. Jelen összefoglaló célja az ischaemiás-reperfúziós károsodás mérséklésére irányuló technikák áttekintése a szerző kutatócsoportjának eddigi munkássága (ischaemiás prekondicionálás, -perkondicionálás, glutaminszupplementáció, adenozin-, inozin-, levosimendan-, poli-ADP-ribóz polimerázgátlás szerepe) alapján, külö-nös tekintettel a máj ischaemiás-reperfúziós károsodására, valamint a szabad gyökök abban betöltött patofi ziológiai szerepére. Orv. Hetil., 2015, 156(47), 1904-1907. Kulcsszavak: ischaemiás-reperfúziós károsodás, máj, szabad gyök, ischaemiás prekondicionálás Free radicals and hepatic ischemia-reperfusionThe critical importance of the ischemic-reperfusive injury is well documented with regards to numerous organs and clinical conditions. Oxygen free radicals play a central role in the mediation of the injury, which dominantly infl uences the prevalence of postoperative complications, (long term) organ damage, and the potential manifestation of systemic reactions. The both anatomically and pathophysiologically unique ischemic-reperfusive injury of the liver, which is expressively vulnerable to free radicals, is of utmost importance in liver surgery. Several techniques (adaptive maneuvers, chemical agents) are known to ameliorate the reperfusive injury. Based on the prior research of the workgroup of the author, the aim of the current article is to overview the set of measures capable of attenuating ischemic-reperfusive injury (ischemic preconditioning, -perconditioning, administration of adenosine, -inosine, -levosimendan, and -poly-ADP-ribose-polymerase inhibitor), with special attention to the ischemic-reperfusive injury of the liver, as well as the special pathophysiological role of free radicals in mediating hepatic damage. Keywords: ischemia-reperfusion injury, liver, free radicals, ischemic preconditioningSzijártó, A. [Free radicals and hepatic ischemia-reperfusion]. Orv. Hetil., 2015, 156(47), 1904-1907. (Beérkezett: 2015 elfogadva: 2015. szeptember 23.) Rövidítések ADP = adenozin-difoszfát; ALP = alkalikus foszfatáz; ALT = alanin-aminotranszferáz; AMP = adenozin-monofoszfát; ATP = adenozin-triszfoszfát; CARS = compensatory anti-infl ammatory response syndrome; CX = (circumfl ex artery) bal szívkoszorúér körbefutó ága; DAMP = danger-associated molecular pattern; DNS = dezoxiribonukleinsav; GSH = glutation redukált forma; I-R = ischaemia-reperfúzió; ic. = intracelluláris; IFN-γ = interferon-γ; IL-1β = interleuk...

show abstract

The Effect of Ischemic Preconditioning Prior to Intraoperative Radiotherapy on Ischemic and on Reperfused Rat Liver

Cited by 7 publications

References 48 publications

Effect of ischemic preconditioning on radiation damage to the submandibular gland in rats

Effect of ischemic preconditioning on radiation damage to the submandibular gland in rats

The effect of the antioxidant drug “U-74389G” on alkaline phosphatase levels during ischemia reperfusion injury in rats

Szabad gyökök és a máj ischaemiás-reperfúziós károsodása

Contact Info

Product

Resources

About