The Effect of Irradiation on Lysosomal Activation in HeLa Cells

Reynolds, Claire; Wills, Eric D.

doi:10.1080/09553007414550131

Cited by 17 publications

(2 citation statements)

References 10 publications

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“…To improve the chromium reduction efficiency, an improved method, which combines ultrasound and microbial reduction of Cr(VI) is presented. The integration of ultrasonic with biological reactions demonstrated that the sonication increases the mass transfer in aqueous solution [16,17], which in turn enhances the bioavailability [18,19]. Gli et al [20] analyzed the effect of ultrasonic irradiation on the reduction of Hg(II) and achieved a maximum reduction of 94%.…”

Section: Introductionmentioning

confidence: 99%

Sonoassisted Microbial Reduction of Chromium

Kathiravan

Karthick

Muthu³

et al. 2009

Appl Biochem Biotechnol

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This study presents sonoassisted microbial reduction of hexavalent chromium (Cr(VI)) using Bacillus sp. isolated from tannery effluent contaminated site. The experiments were carried out with free cells in the presence and absence of ultrasound. The optimum pH and temperature for the reduction of Cr(VI) by Bacillus sp. were found to be 7.0 and 37 degrees C, respectively. The Cr(VI) reduction was significantly influenced by the electron donors and among the various electron donors studied, glucose offered maximum reduction. The ultrasound-irradiated reduction of Cr(VI) with Bacillus sp. showed efficient Cr(VI) reduction. The percent reduction was found to increase with an increase in biomass concentration and decrease with an increase in initial concentration. The changes in the functional groups of Bacillus sp., before and after chromium reduction were observed with FTIR spectra. Microbial growth was described with Monod and Andrews model and best fit was observed with Andrews model.

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Section: Introductionmentioning

confidence: 99%

Sonoassisted Microbial Reduction of Chromium

Kathiravan

Karthick

Muthu³

et al. 2009

Appl Biochem Biotechnol

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“…Cortisone acetate also reduces cytolysis and enhances the growth of herpes simplex virus (23). Since the growth of Rickettsia typhi in the cytoplasm of previously irradiated L cells has been studied extensively in this laboratory (27) and irradiation tends to activate the lysosomes (17), it appeared of interest to determine whether the yield ofrickettsiae can be increased by lysosomal stabilization.…”

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confidence: 99%

Growth of Rickettsia typhi in irradiated L cells enhanced by lysosomal stabilization

Woodman¹,

Schultz²,

Woodman³

et al. 1979

Infect Immun

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The growth of some obligate intracellular parasites is contingent upon avoidance of lysosomal activation during growth in their host cells. This is accomplished by the various parasites by different mechanisms and with different degrees of efficiency. The possibility was tested that the lysosomal stabilizer cortisone acetate might protect and thus enhance the growth of Rickettsia typhi in mouse L cells irradiated 6 days earlier. Beginning 2 days before infection of the L cells with a multiplicity of 10 rickettsiae, 20 microgram of cortisone per ml was added in medium 199 containing 5% fetal calf serum. This concentration of cortisone was below the cytotoxic level, as determined by viability staining, but was sufficient to significantly alter the ratios of cellular and released acid phosphatase and beta-glucuronidase in uninfected and infected cells, as shown by spectrophotometric analysis. Rickettsial replication, measured by hemolytic activity at 96 h and confirmed by microscopic observations at earlier stages of infection, was increased by the cortisone. Cortisone concentrations of 10 or 40 microgram/ml were less effective, and cortisone was ineffective when the rickettsial multiplicity per L cell was 2 or lower. These results indicate that amounts of cortisone that increase lysosomal stabilization in L cells favor rickettsial multiplication when the multiplicity of infection is relatively high.

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Absence of arylsulphatases A and B in suction blister fluid interstitial fluid and serum

Volden

1977

Arch. Derm. Res.

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Suction blisters were raised on human skin. The blister fluid was collected for enzymic assay and the epidermis over the bullae was removed before further suction of interstitial fluid. Preliminary work indicated the presence of arylsulphatases A and B in epidermis, and the same assay procedure with 4-methylumbelliferyl sulphate as substrate was used. There appeared to be no enzymatic activity in blister fluid, interstitial fluid and serum.

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The Effect of Irradiation on Lysosomal Activation in HeLa Cells

Cited by 17 publications

References 10 publications

Sonoassisted Microbial Reduction of Chromium

Sonoassisted Microbial Reduction of Chromium

Growth of Rickettsia typhi in irradiated L cells enhanced by lysosomal stabilization

Absence of arylsulphatases A and B in suction blister fluid interstitial fluid and serum

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