The Effect of Iron and Fat in a Diet Containing Green Tea Extract (Camellia sinensis) on the Antioxidant Capacity of Some Organs and the mRNA Expression of Specific Genes in Mice

Abstract

The hypothesis that iron and fat in the diet may affect green tea extract (GTE) bioactivity, in particular antioxidant capacity and gene expression, was proposed and tested in mice. Thirty mice were randomly assigned to have for 37 days free access to standard or high-fat diets with or without GTE and ferrous lactate. Mice were euthanized and specific organs were removed. Total antioxidant capacity (TAC) was measured using the ferric reducing antioxidant power (FRAP) and oxygen radical absorbance capacity assays. Polymerase chain reaction was performed on liver and heart mRNA extracts. The FRAP assay showed that GTE from the standard diet did not affect plasma TAC but increased TAC of heart, aorta, and duodenum. GTE from diets enriched with iron resulted to lower TAC of liver and heart than diets with GTE alone. GTE from the fatty diet did not have any effect on TAC compared with fatty control diet, but increased TAC in heart and aorta compared with standard control diet. An effect on expression of the mapk-1 and NF-kB genes in heart was observed in the presence of GTE. These results suggest that GTE may exhibit bioactivity in some organs affected by dietary fat and iron. The findings of this study contribute to the elucidation of the role of dietary components on tea bioactivity.

Combined treatment of 3-hydroxypyridine-4-one derivatives and green tea extract to induce hepcidin expression in iron-overloaded β-thalassemic mice

“…A current study has supported that the combined chelation treatment synergized mobilization of labile iron pools in Huh7 cells, in which the DFX + DFP treatment was the most effective [45]. Though effect of green tea consumption on iron absorption is not conclusive definitely, we believe that it will not affect our study [46,47].…”

Pure Polyphenols and Cranberry Juice High in Anthocyanins Increase Antioxidant Capacity in Animal Organs

“…The difference in AC between brain and the other organs was highly significant ( p < 0.001) and was probably a result of the blood-brain barrier limiting PP accumulation. In another study, the brain also had the lowest AC among the organs in a rats given a green tea extract high in catechins and other PP [15]. Another analysis showed that FRAP AC of brain tissue was lowest compared to the other organs tested [16].…”

Association of Mean Daily Polyphenols Intake with Mediterranean Diet Adherence and Anthropometric Indices in Healthy Greek Adults: A Retrospective Study

“…Dietary polyphenols have been studied for their potential beneficial effect on human health and disease prevention [2,3,[8][9][10][11][12][13][14][44][45][46][47]. Nevertheless, their bioactivity is dependent on their bioavailability, which differs in the various polyphenol subcategories [15][16][17].…”

“…Dietary models rich in fruits, vegetables, cereals, legumes, nuts, and a plethora of natural functional foods and beverages, are linked with high intake of polyphenols [4][5][6][7][8][9]. Their structure, with hydroxyl groups, promotes their possible antioxidant activity in human organisms, while antibacterial, anti-inflammatory, cardioprotective, antidiabetic, and anticancer activities have also been studied due to either antioxidative properties or possible effects on gene expression, by modulating protein synthesis and specific metabolic pathways related to diseases' pathophysiology [2,3,[8][9][10][11][12][13][14].…”