The effect of Iridoids effective fraction of Valeriana jatamansi Jones on movement function in rats after acute cord injury and the related mechanism

Abstract

Objectives Spinal cord injury (SCI) is a disastrous central nervous system (CNS) disorder, which was intimately associated with oxidative stress. Studies have confirmed that Iridoids Effective Fraction of Valeriana jatamansi Jones (IEFV) can scavenge reactive oxygen species. This study aimed to confirm the efficacy of IEFV in ameliorating SCI. MethodsFor establish the SCI model, the Sprague-Dawley rats underwent a T10 laminectomy with transient violent oppression by aneurysm clip. Then, the rats received IEFV intragastrically for 8 consecutive weeks to evaluate the protective effect of IEFV on motor function, oxidative stress, inflammation and neurotrophic factors in SCI rats.Results Basso, Beattie and Bresnahan scores, hematoxylin and eosin (H&E) staining and transmission electron microscopy experiments found IEFV protected motor function and alleviated neuron damage. Meanwhile, IEFV treatment decreased the release of malondialdehyde, interleukin-6 (IL-6), cyclooxygenase-2 and tumor necrosis factor-α . Moreover, IEFV treatment elevated the expression levels of brain-derived neurotrophic factor and nerve growth factor of SCI rats. Finally, administration of IEFV significantly inhibited the expression of p-p65 and toll-like receptor 4 (TLR4). ConclusionsThis study suggests that IEFV could attenuate the oxidative stress and inflammatory response of the spinal cord after SCI, which was associated with inhibition of the TLR4/nuclear factor-kappaB signaling pathway.

Iridoids rich fraction from Valeriana jatamansi Jones promotes axonal regeneration and motor functional recovery after spinal cord injury through activation of the PI3K/Akt signaling pathway

“…Furthermore, it is devoid of toxicological side effects and evident drug dependence ( Yu et al, 2015 ). Our previous research has established that IRFV can enhance motor function recovery and elevate the expression levels of brain-derived neurotrophic factor and nerve growth factor in SCI rats ( Xiong et al, 2022 ). In this study, aligning with prior findings, our results corroborate that IRFV enhances motor function in SCI rats.…”

“…Renowned for its analgesic, antidiarrheal, and sedative properties, it holds a venerable position in herbal medicine ( Wang et al, 2018 ). Our preceding research revealed that the Iridoid-rich fraction from Valeriana jatamansi Jones (IRFV) significantly enhances motor function recovery following SCI in rat models, reduce inflammatory response, and promote the expression of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF), with the optimal efficacy noted at a dosage of 10 mg/kg ( Xiong et al, 2022 ).…”

Mechanism of Valeriana Jatamansi Jones for the treatment of spinal cord injury based on network pharmacology and molecular docking

“…In recent years, VJJ has displayed an effect on the central nervous system through various molecular mechanisms [ 42 ] and demonstrated improvement in the recovery of motor function in rats after SCI. [ 11 ] The study adopted a network pharmacological approach to predict the molecular mechanism of VJJ in SCI and added molecular docking techniques to provide a new idea and theoretical basis for further in-depth investigation of the application of VJJ in SCI treatment.…”

“…[ 9 , 10 ] VJJ is a Chinese herbal medicine with promising future development. Additionally, VJJ can promote the recovery of hind limb motor function in rats with acute SCI, [ 11 ] counteract oxidative stress caused by SCI, [ 12 ] and ease neuronal apoptosis and pyroptosis, [ 13 ] all of which have neuroprotective effects. [ 14 ] However, the specific molecular mechanism of action of VJJ in SCI remains unclear.…”