The effect of ionizing irradiation on the lipid composition of the liver mitochondria of rats

Schwarz, H. P.; Dreisbach, L.; Barrionuevo, M.; Kleschick, A.; Kostyk, I.

doi:10.1016/0003-9861(61)90227-2

Cited by 26 publications

(2 citation statements)

References 18 publications

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“…The large amount of polyglycerophosphatide in brown fat is of particular interest, since these substances appear t o be concentrated in the mitochondria of tissues (2 1). T h e polyglycerophosphat ide level of brown fat is comparable with t h a t of rat liver mitochondria, which contain most of the polyglycerophosphatide of liver (21,23). T h e brown fat cells contain numerous mitochondria which are responsible for much of the brown color of the tissue.…”

Section: Similarities Of Phosphatides Of Brown Fat and Livermentioning

confidence: 89%

The Lipids of Mouse Brown Fat

Spencer

Dempster

1962

Can. J. Biochem. Physiol.

107

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The lipids of the interscapular brown fat of the white mouse have been examined by means of silicic acid column chromatography and quantitative paper chromatography. The fatty acid composition of the glycerides and phosphatides was determined by gas chromatography. Comparative studies of mouse brown and white fat are presented.The fatty acid composition of the glycerides of the two tissues was similar a t all ages examined. In both tissues, the average chain length of the glyceride fatty acids increased with age. The glycerides of brown fat a t all ages contained less palmitoleic acid and more stearic acid than the corresponding white fat.The adult brown fat contained more polyglycerophosphatide and less sphingomyelin than white fat. The fatty acid composition of the lecithins of the two tissues was very similar. The similarity of brown fat and liver phosphatides is discussed.Studies on the brown fat, or "hibernating gland", have been reviewed by Johansson (1). T h e lipids have been examined mainly by histochemical techniques. LaChance (2) measured the total lipid content of the interscapular fat of growing rats. Langer and Langer-Schierer (3, 4) measured the total lipid and lipid phosphorus in the brown fat of European hamsters and growing rats. T o our knowledge, no detailed study of the phosphatides of brown fat has been made.T h e present study is part of an extended project t o study the host-virus relationship in experimental infection of a number of hibernating and nonhibernating species (S), with particular reference t o the susceptibility of brown fat.

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Section: Similarities Of Phosphatides Of Brown Fat and Livermentioning

confidence: 89%

The Lipids of Mouse Brown Fat

Spencer

Dempster

1962

Can. J. Biochem. Physiol.

107

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“…For qualitative assay of sugars and hexosamines the glycosphingolipids were hydrolyzed with 2 N H,SO, as described by Schwarz et al [32]. For the subsequent separation the thin-layer procedure of von Berg [33] was applied on cellulose ready plastic sheets (Schleicher u. Schull, Dassel, Germany) ; sugars were located by spraying with aniline phthalate reagent, hexosamines by subsequent spraying with ninhydrin reagent and heating a t 110".…”

Section: Thin-layer Chromatographymentioning

confidence: 99%

The J Blood-Group Substance of Cattle. J Haptenic Activity of Glycosphingolipids of Serum

Thiele

Koch

1970

Eur J Biochem

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The bovine J blood-group substance is dissolved in the serum. The total lipids extracted from serum containing the J antigen (termed J+ serum) show haptenic activity in the homologous bovine J system. The total lipids from J-serum, however, show no haptenic activity. The J+ total lipids are, therefore, thought to contain the determinant group of the J antigen.I n fractionation experiments the lipids to be tested were mixed with crude glycerophospholipids extracted from J-serum, since it has been known that auxiliary lipids are required for maximum haptenic effects. The haptenic activity is completely destroyed by periodate oxidation, it does not decrease, however, by deacylation of ester lipids. Various fractionation procedures in addition to deacylation led to a purified glycosphingolipid that proved to be highly active as a J hapten.The low-density lipoproteins prepared from J+ serum as well as the total lipids extracted from these lipoproteins inhibit the hemolysis of J+ erythrocytes in the J system.These results suggest that the J blood-group substance dissolved in bovine J+ serum is a lipoprotein and that its determinant group is a glycosphingolipid.The bovine J blood-group system [1,2] plays a special role among the different blood-group systems of cattle. I n contrast to other blood-group systems the J substance is primarily dissolved I n this paper the serum or erythrocytes containing the J substance are called J f , the serum lacking the J substance is called J-.The J substance corresponds to a naturally occuring antibody (anti-J) that may or may not be found in J-serum. There are differences in the reactibility of the anti-J sera of different animals. Some anti-J sera react more frequently with J+ erythrocytes than others do. Thus. the presence of J subgroups has been suggested. An international terminology of bovine J subgroups, however, has not been intro- duced so far. I n the experiments described in this paper we used J+ serum throughout which has been tested with anti-J sera that had been checked in international comparison tests ; these anti-J sera show the most frequent reactions with J+ erythrocytes. Several years ago we found [5] that the total lipids prepared from J+ serum exhibits haptenic activity in the homologous J system, whereas the corresponding total lipids prepared from J-serum did not exhibit any haptenic activity in the J system. Our previous results have only been described in short communications or abstracts [5-71. This paper presents detailed evidence for the glycosphingolipid nature of the J hapten. MATERIAL AND METHODSBovine J+ and J-sera were obtained from the slaughter-house. The blood-groups had been known prior to the sacrifice of cattle. All experiments described in Results were carried out in duplicates.The various J+ (and J-) serum batches are termed batch I-V. All batches were obtained from one animal each, except batch 11, which was pooled from the blood of several animals. The sera No. 1-111 were kept in a frozen state prior to use, the sera No.IV and V were used f...

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Radiation-Induced Injury of the Liver

Lesch¹

1976

Experimental Production of Diseases

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The effect of ionizing irradiation on the lipid composition of the liver mitochondria of rats

Cited by 26 publications

References 18 publications

The Lipids of Mouse Brown Fat

The Lipids of Mouse Brown Fat

The J Blood-Group Substance of Cattle. J Haptenic Activity of Glycosphingolipids of Serum

Radiation-Induced Injury of the Liver

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