The effect of ionic strength and phosphate ions on the construction of redox polyelectrolyte–enzyme self-assemblies

Abstract

The type and concentration of ions present in a solution containing an electroactive polyelectrolyte shape its configuration, adsorption, and electrochemical response.

Current Response Enhancement According to the Doping Anion’s Nature in Redox Polyelectrolyte─Enzyme Assemblies

“…It is important to highlight that for both redox polyelectrolytes, the presence of bromide in the construction process yields systems with higher catalytic currents, leading to the conclusion that GOx adsorption is heavily dependent on the hydrophobic character of the precedent layer. Also, the OsPAA ion doping has a striking effect on graphite electrodes in the adsorption process and in the catalysis; the current observed here, 240 μA cm –2 , is 2.5 times higher than those observed with OsPAA in the presence of phosphate, gold nanoparticles, surfactants, and their combinations. ,, …”

“…The presence of chloride, fluoride, and bromide ions at the same concentration was evaluated in OsPAA solutions used for OsPAA/GOx assemblies to compare the effect of these ions on a linear redox polyelectrolyte. OsPAA (Figure B) has been widely studied in assemblies with enzymes, incorporating metallic nanoparticles, surfactants, and phosphate ions. − …”

“…Also, the OsPAA ion doping has a striking effect on graphite electrodes in the adsorption process and in the catalysis; the current observed here, 240 μA cm −2 , is 2.5 times higher than those observed with OsPAA in the presence of phosphate, gold nanoparticles, surfactants, and their combinations. 33,34,41 Finally, the use of enzyme-redox hydrogel modified electrodes as bioanodes in enzymatic fuel cells has shown outstanding performance for this type of fuel cell, mainly devoted to self-powered implantable medical devices. In this type of cell, a size reduction is extremely important.…”

Transduction of Amine–Phosphate Supramolecular Interactions and Biosensing of Acetylcholine through PEDOT-Polyamine Organic Electrochemical Transistors

“…Similarly, poly(allylamine)-Pi (PAH-Pi) nanoparticles have been used as carriers for silencing RNAs . Furthermore, polyamine–phosphate interactions have been used to create supramolecular films which can modulate cell adhesion and proliferation, or integrate redox enzymes for biosensing applications …”

“…17 proliferation, 18 or integrate redox enzymes for biosensing applications. 19 On the other hand, organic electrochemical transistors (OECTs) have emerged as a promising class of devices for bioelectronic applications due to their unique properties such as high interfacial sensitivity and offering scalable manufacturing of flexible and miniaturized sensing arrays. 20−22 These devices consist of an organic semiconductor film that spans source and drain electrodes and whose conductivity can be regulated by the application of a gate voltage (V G ) through an electrolyte.…”