The effect of intracytoplasmic sperm injection and semen parameters on blastocyst development in vitro

Purpose: The aim was to establish the impact of human granulosa cell apoptosis and reactive oxygen species (ROS) production on fertilization competence of the oocyte, embryo developmental stage and implantation rate.Methods: Thirty women undergoing IVF-ET for tubal factor infertility were included; GnRH antagonists and gonadotrophins were used for ovarian stimulation. Granulosa cells were isolated from each aspirated follicle using gradient centrifugation. Apoptosis was studied by flow cytometry using annexin V and propidium iodide. ROS production was studied with hydroethidine staining and analyzed by flow cytometry.Results: There were no differences in characteristics of granulosa cells between the follicles with fertilized and nonfertilized oocytes. The analyzed characteristics of granulosa cells in corresponding follicles had no effect on embryo developmental stage on day 5. The percentage of ROS producing granulosa cells was lower in the follicles giving rise to blastocysts that resulted in implantation compared to those that did not (39.9% versus 69.9%, P = 0.031).Conclusions: Apoptosis and ROS production in granulosa cells have no significant impact on fertilization and do not correlate with the development of blastocysts. An increased

Section: Discussionmentioning

confidence: 99%

Effect of apoptosis and reactive oxygen species production in human granulosa cells on oocyte fertilization and blastocyst development

Jančar

Kopitar

Ihan

et al. 2007

“…Since impaired semen quality may have a negative impact on the development of embryos, the influence of ICSI on blastocyst development is still a matter of debate. Some studies reported decreased blastocyst development [14,29] in ICSI than IVF embryos whereas others did not observe any difference [22,30]. Rienzi et al reported blastocyst formation rate as 44.8 % in ICSI cycles [30].…”

Section: Discussionmentioning

confidence: 98%

Comparison of the transfer of equal numbers of blastocysts versus cleavage-stage embryos after repeated failure of in vitro fertilization cycles

Karacan¹,

Ulug²,

Arvas³

et al. 2013

Purpose To evaluate the efficacy of blastocyst transfer in women with at least two previously unsuccessful in vitro fertilization-embryo transfer (IVF-ET) attempts. Methods Retrospective analysis of 238 couples (with previous implantation failures) had equal number (two) of cleavage-stage embryos (n =143) or blastocysts (n =95) transferred in the same IVF center.Results The clinical pregnancy rates and live-birth rates were similar in the cleavagestage embryo transfer group and the blastocyst group (35.6 % vs. 40 % and 32.1 % vs. 35.7 %; p >0.05, respectively). Miscarriage rates (9.8 % vs. 10.5 %) and multiple pregnancy rates (15.6 % vs. 23.6 %) did not differ. Although implantation rate was higher with blastocyst transfer than that with day 3 transfer, it did not reach to a statistical significance (24.7 % and 19 %, respectively, p >0.05). Conclusion Blastocyst transfer in ICSI cycles does not yield a better outcome than that obtained with cleavage-stage embryos in women who had unsuccessful IVF attempts previously.

“…It has been demonstrated that oocytes injected with spermatozoa with high motility have a significantly higher chance of reaching the blastocyst stage [30,43]. It has also been reported that paternal cigarette smoke exposure affects the embryonic implantation ability [21].…”

Section: Discussionmentioning

confidence: 99%

Patient selection criteria for blastocyst transfers in extended embryo culture programs

Braga

Setti

Figueira

et al. 2012

Purpose To identify the correlation between different cycles, patient factors and blastocyst characteristics. Methods The study included 420 patients undergoing ICSI cycles and 2781 graded blastocysts, which took into account the blastocyst quality. The correlations between the blastocyst parameters and the patient and cycle characteristics were assessed. Results The blastocyst development was negatively correlated with the maternal age, BMI and dose of FSH. The ICM was negatively correlated with the FSH dose, whereas the TE quality was influenced by the FSH dose, the maternal age and the number of retrieved oocytes. The embryo morphology on days two and three may predict the blastocyst developmental competence. Conclusions Older patients and patients with high BMI should not be included in extended embryo culture programmes. The extended culture may not favour embryos with poor morphology on days two and three of development. Additionally, a lower ovarian stimulation and decreased oocyte yields may lead to the development of high-quality blastocysts.