The effect of intracisternal administration of hyaluronidase on the body of cattle

Abstract

The aim of the research was to determine the magnitude of the irritant effect on breast tissue and the morphobiochemical composition of blood in lactating cows with a single intracisternal administration of hyaluronidase. To do this, 1,280 units of hyaluronidase prepared in 10 ml of 0.9% sodium chloride solution were injected once into the posterior right quarter of the experimental group (n=10). The first heifers of the control group (n = 10) received a saline solution in its pure form. Before and after the administration of the drugs, the electrical conductivity and concentration of somatic cells in milk were determined (by the viscometric method), as well as the morphobiochemical composition of blood was studied. It was found that 12 hours after the introduction of the enzyme, the electrical conductivity of the secretion decreased by 7.6% (P ≤ 0.05), and after the first day it was completely restored to its initial values. The dynamics of the level of somatic cells 6 and 12 hours after the application of hyaluronidase in 40% of animals was characterized by values above 500 thousand / ml, and the average concentration of cells increased by 3 times. After the first and second days, the level of inflammatory reaction cells decreased by 2.0...2.5 times, relative to peak values. After 72 hours, the cell content in the secret decreased by 2.8 times in relation to the values obtained after 6 hours and by 27.7% in relation to the values established a day after the application of hyaluronidase, and did not significantly differ from the indicators before the introduction of the enzyme. The use of the drug did not significantly affect the morphobiochemical parameters of the blood of experimental animals. Thus, a single intracisternal administration of 1280 units of hyaluronidase to lactating cows is accompanied by a slight and short-term irritating effect on udder tissue, which allows further studies of this drug in the pharmacocorrection of breast pathologies.