The Effect of Interleukin-8 on the Viability of Injected Adipose Tissue in Nude Mice

Abstract

Adipose tissue injection as a free graft for the correction of soft-tissue defects is a widespread procedure in plastic surgery. The main problem in achieving long-term soft-tissue augmentation is partial absorption of the injected fat and hence the need for overcorrection and re-injection. The purpose of this study was to improve the viability of the injected fat by the use of interleukin-8. The rationale for the use of interleukin-8 was its abilities to accelerate angiogenesis and attract inflammatory cells and fibroblasts, providing the injected adipocytes more feeding vessels and a well-established graft bed to enhance their viability. Human adipose tissue, obtained by suction-assisted lipectomy, was re-injected into the subcutis in the scalp of nude mice. Interleukin-8 (0.25 ng) was injected subcutaneously to the scalp as a preparation of the recipient site 24 hours before the fat injection and was added to the fat graft itself (25 ng per 1 cc of injected fat). In the control group, pure fat without interleukin-8 was injected and no interleukin-8 was added for the preparation of the recipient site. One cubic centimeter of fat was injected in each animal in both the study and control groups. There were 10 animals in each group. The animals were euthanized 15 weeks after the procedure. Graft weight and volume were measured and histologic evaluation was performed. In addition, triglyceride content and adipose cell sizes were measured as parameters for fat cells viability. Histologic analysis demonstrated significantly less cyst formation in the group treated with interleukin-8. No significant differences were found between the groups with regard to graft weight and volume or the other histologic parameters investigated. No significant differences were demonstrated in adipose cell sizes and their triglyceride content. In conclusion, less cyst formation, indicating improved quality of the injected fat, can be obtained by the addition of interleukin-8. Further studies of various dosages of interleukin-8 and their long-term effect are required before these encouraging results could be applied clinically.

Angiogenesis modulates adipogenesis and obesity

“…ingly, IL-8 could be a survival factor for adipocytes in vivo, probably via stimulation of angiogenesis (89). Thus inflammatory cells in growing adipose tissues are coordinators for coupling the concomitant events of adipogenesis and angiogenesis.…”

An Alternative Method for Harvest and Processing Fat Grafts: An In Vitro Study of Cell Viability and Survival

“…Among several techniques suggested for improving fat viability are lowpressure harvesting, washing, centrifugation, and fat concentration in dense cotton; and treatment with substances that range from growth factors to growth medium. [12][13][14][15][16][17][18][19][20][21] In 2001, Jackson et al reported on the use of a fine-needle aspiration apparatus connected to a 2-mm blunt needle for fat harvesting and the towel-drying technique to process the fat for implantation. 5 With this method and a gentle technique for handling the fat, the authors reported maintenance of the fat graft for more than 2 years.…”

Coenzyme Q10 Does Not Enhance Preadipocyte Viability in an In Vitro Lipotransfer Model

“…Shoshaniet al [30] investigated the influence of lidocaine and adrenaline on the viability of injected adipose tissue in nude mice. No influence of lidocaine and adrenaline could be detected.…”