The effect of insulin on maturation and development of -fertilized bovine oocytes

Zhang, L.; Blakewood, E.G.; Denniston, R.S.; Godke, R.A.

doi:10.1016/0093-691x(91)90277-k

Cited by 28 publications

(10 citation statements)

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“…Inconsistent results have been reported after the addition of different concentrations of insulin during either follicular culture (Fouladi-Nashta and Campbell 2006), oocyte maturation (Zhang et al 1991;Bowles and Lishman 1998), fertilisation (Bowles and Lishman 1998) or embryo culture (Matsui et al 1995(Matsui et al , 1997Mihalik et al 2000). The contradictory results reveal the complexity of the insulin-IGF system and characterise the difficulties in establishing an in vitro model for metabolic imbalance caused by elevated insulin concentrations.…”

Section: Introductionmentioning

confidence: 95%

“…Diverse in vitro studies have investigated effects of insulin on early embryonic development in different species, such as the mouse (Rao et al 1990), rabbit (Herrler et al 1998), rat (Zhang et al 1990), pig (Lewis et al 1992) and bovine (Zhang et al 1991;Matsui et al 1995;Bowles and Lishman 1998). Inconsistent results have been reported after the addition of different concentrations of insulin during either follicular culture (Fouladi-Nashta and Campbell 2006), oocyte maturation (Zhang et al 1991;Bowles and Lishman 1998), fertilisation (Bowles and Lishman 1998) or embryo culture (Matsui et al 1995(Matsui et al , 1997Mihalik et al 2000).…”

Section: Introductionmentioning

confidence: 97%

“…In bovine, in vivo concentrations of insulin in the serum and follicular fluid range between approximately 0.1 and 0.5 ng mL À1 depending on diet and energy state (Landau et al 2000;Gong et al 2002;Freret et al 2006;Shimizu et al 2008;Garnsworthy et al 2009) and even follicular phase (Landau et al 2000). The difficulty in choosing adequate insulin concentrations in an in vitro model is related to differences between in vitro and in vivo maturation (Leibfried-Rutledge et al 1987;Rizos et al 2002) and the fact that insulin has been reported to be unstable in in vitro culture systems, with activity starting at concentrations above 1 mg mL À1 (Hayashi et al 1978), which explains why concentrations of insulin used in vitro tend to be higher (usually in the microgram per millilitre range) compared with in vivo concentrations (Zhang et al 1991;Matsui et al 1995Matsui et al , 1997Bowles and Lishman 1998). Conversely, it has been reported that bovine insulin in physiological concentrations (0.1 and 1 ng mL À1 ) during granulosa cell culture leads to increased FSH-induced oestradiol production, with a decreasing effect if insulin is used at higher doses (10, 100 or 1000 ng mL À1 ; Spicer and Echternkamp 1995).…”

Section: Introductionmentioning

confidence: 99%

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Insulin exposure during in vitro bovine oocyte maturation changes blastocyst gene expression and developmental potential

Laskowski

Humblot

Sirard

et al. 2017

Reprod. Fertil. Dev.

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Abstract. Metabolic imbalance impairs fertility, because changes in concentrations of metabolites and hormones in the blood and follicular fluid create an unfavourable environment for early embryonic development. Insulin is a key metabolic hormone known for its effects on fertility: insulin concentrations are increased during energy balance disturbances in diabetes or metabolic syndrome. Still, insulin is frequently used at supraphysiological concentrations for embryo in vitro culture with unknown consequences for the developmental potential of the offspring. In the present study we investigated the effects of insulin exposure during in vitro bovine oocyte maturation on developmental rates, embryo quality and gene expression. Supplementation of the maturation media with insulin at 10 or 0.1 mg mL À1 decreased blastocyst rates compared with an insulin-free control (19.8 AE 1.3% and 20.4 AE 1.3% vs 23.8 AE 1.3%, respectively; P , 0.05) and led to increased cell numbers (nearly 10% more cells on Day 8 compared with control; P , 0.05). Transcriptome analysis revealed significant upregulation of genes involved in lipid metabolism, nuclear factor (erythroid-derived 2)-like 2 (NRF2) stress response and cell differentiation, validated by quantitative polymerase chain reaction. To conclude, the results of the present study demonstrate that insulin exposure during in vitro oocyte maturation has a lasting effect on the embryo until the blastocyst stage, with a potential negative effect in the form of specific gene expression perturbations.

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Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Insulin exposure during in vitro bovine oocyte maturation changes blastocyst gene expression and developmental potential

Laskowski

Humblot

Sirard

et al. 2017

Reprod. Fertil. Dev.

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“…Although data are scant, the role of insulin and IGFs is likely to be important in farm animal species as well. In pig embryos, in fact, insulin increases P-450 aromatase activity (Hofig et al, 1991) and stimulates the embryonic disk to synthesise four new proteins which may be involved in maternal recognition of pregnancy (Estrada et al, 19911, while in cows, the hormone enhances the development of in vitro fertilised oocytes (Zhang et al, 1991).…”

Section: Primatesmentioning

confidence: 99%

Functions of proteins secreted by oviduct epithelial cells

Gandolfi

1995

Microscopy Res & Technique

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Studies on embryonic development in vitro as well as observations in vivo, suggested that two aspects of oviduct physiology are important for early development. On one side has to be considered the oviduct "environment": temperature, pH, osmotic pressure, nutrients, oxygen tension, free radical scavengers, etc. On the other, the oviduct "active components": stimulatory and/or regulatory molecules, supposed to finely regulate the fertilisation process and the first differentiative steps. While the physical environment of the oviduct has been under investigation for some decades, studies on oviduct-specific molecules and their functions have only been developed much more recently. The amount of information on this topic, however, has rapidly reached the size that demands a summary. In this review the descriptive literature on oviduct specific proteins will be examined as a basis for illustrating the possible functions of these molecules. In particular their role in fertilisation and early embryonic cleavages will be analysed in some details. Finally a section is devoted to the presence and physiological significance of growth factors in oviduct fluid.

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“…In order to complete nuclear as well as cytoplasmic maturation in an in vitro culture system, several components such as follicle stimulating hormone, luteinizing hormone, estradiol, the presence of cumulus cells and a serum source must all be considered. Maturation of oocytes for in vitro fertilization (IVF) has been accomplished in culture medium supplemented with estrous cow serum (ECS) (Lu et al, 1987(Lu et al, ,1988, fetal bovine serum (FBS) (Goto et al, 1988;Zhang et al, 1991), as well as in either gonadotropin and steroid supplemented ECS or FBS (Sirard et al, 1988;Younis et al, 1989;Schellander et al, 1991;Threlfall, 1989, 1990). After IVF, embryo co-culture with cumulus cells (Kajihara et al, 19901, granulosa cells (Yang and Lu, 19901, and oviduct cells (Eyestone and First, 1989) have been used to provide the necessary culture environment so that embryos may develop to advanced preimplantation stages for eventual nonsurgical transfer to recipients and/or cryopreservation for storage.…”

Section: Introductionmentioning

confidence: 99%

Effects of maturation and co‐culture treatments on the developmental capacity of early bovine embryos

Wiemer

Watson

Polanski

et al. 1991

Molecular Reproduction Devel

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A total of 901 cumulus-oocyte complexes (COCs) were collected from bovine ovaries obtained at a local abattoir. COCs randomly assigned to Treatment I (n = 451), were cultured in TCM-199 + 10% fetal bovine serum (FBS) and hormones, while oocytes in Treatment II (n = 450) were cultured in TCM-199 + 20% estrous cow serum (ECS). Assessment of maturation revealed that 91.3% (42/46) of oocytes in Treatment I had reached metaphase II of meiosis, which was greater (P less than 0.05) than the 73.3% (33/45) in Treatment II. Following in vitro fertilization, 203 oocytes from Treatment I were co-cultured on bovine granulosa cells (Treatment IA) while the remaining 202 oocytes were co-cultured on bovine oviductal cells (Treatment IB). Similarly, 203 oocytes from Treatment II were co-cultured on granulosa cells (Treatment IIA) or oviductal cells (Treatment IIB, n = 202). Co-culture was maintained for 8 days. The proportion of cleaved zygotes was higher (P less than 0.05) in Treatment IB (86.6%) compared to Treatments IA (78.8%), IIA (58.1%), and IIB (64.8%). The proportion of cleaved zygotes that progressed beyond the 16-cell stage was also greater (P less than 0.001) in Treatment IB (71.4%) compared to Treatments IA (50.0%), IIA (35.4%) and IIB (55.8%). Treatment IB also produced the highest proportion of blastocysts (P less than 0.0001) (41.1%) versus 24.6% (IA), 11.3% (IIA) and 18.3% (IIB). The proportion of day 6 morulae that progressed to form day 8 blastocysts was similar for both co-culture treatments (IA, 70.1%; IB 70.2%; IIA, 51.5%; IIB 50.8%) and varied only between in vitro maturation groups.(ABSTRACT TRUNCATED AT 250 WORDS)

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The effect of insulin on maturation and development of -fertilized bovine oocytes

Cited by 28 publications

References 0 publications

Insulin exposure during in vitro bovine oocyte maturation changes blastocyst gene expression and developmental potential

Insulin exposure during in vitro bovine oocyte maturation changes blastocyst gene expression and developmental potential

Functions of proteins secreted by oviduct epithelial cells

Effects of maturation and co‐culture treatments on the developmental capacity of early bovine embryos

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