The effect of inorganic and organically bound forms of selenium on glutathione peroxidase activity in the blood of goats

Abstract

The goal of the experiment was to compare the effect of supplementation of inorganic and the new organically bound (lactate-protein selenium complex) form of selenium (Se) in feed for goats. The 31 goats were split into three groups: control (C) without Se supplementation, AN group administered sodium selenite, ORG group administered lactate-protein selenium complex (Selene Chelate, Agrobac, Czech Republic) produced by cultivation of Lactobacillus acidophilus on a substrate containing natrium selenite. The total Se intake in goats was 0.15 mg in group C, and 0.43 mg in the groups AN and ORG. The effect of Se supplementation was assessed based on the determination of Se concentration and the activity of glutathione peroxidase (GSH-Px) in whole blood. Samples were taken before the beginning of Se supplementation, 14 and 30 days after the start of supplementation, and then two and three months after the beginning of supplementation. Average Se concentrations in the blood of goats in individual groups (C, AN, ORG) before the start of supplementation were 109.6 ± 34.3, 117.5 ± 34.7, and 105.4 ± 43.6 μg/l respectively, and the activity of GSH-Px in whole blood was 745.3 ± 289.2, 810.7 ± 280.4, and 791.0 ± 398.1 μkat/l respectively. While in group C goats neither the Se concentration nor the GSH-Px activity changed substantially during the experiment, in the goats in the experimental groups there was a statistically significant increase (P < 0.01) in both Se concentrations and the GSH-Px activities. At the end of the experiment Se concentrations in the blood of AN and ORG groups amounted to 168.5 ± 12.2 and 168.8 ± 26.8 μg/l. The GSH-Px activities in goats supplemented with Se also increased significantly over the course of the experiment (at the end of the experiment it was 1178.0 ± 127.3 in the AN group and 1030.1 ± 152.3 μkat/l in the ORG group), and the difference between the groups was significant (P = 0.038). Regarding the dynamics of GSH-Px activity changes during the monitored period, a markedly quicker increase in GSH-Px activity was recorded in the AN group – one month after the beginning of Se supplementation, compared to three months after the beginning of Se supplementation in the ORG group. The results thus show that the effects of supplementation with selenite and the lactate-protein selenium complex are similar with regard to Se status, but that the increase in GSH-Px activity occurred much faster with selenite, which therefore appears to be a more biologically available form of selenium for creation of biologically active selenoproteins.

Impact of long-term supplementation of zinc and selenium on their content in blood and hair in goats

“…Also, in this case the group supplemented with the lactate-protein complex form reached the highest mean values (872.3 μkat/l) and the group supplemented with the selenite exhibited the lowest activity of this enzyme of all supplemented groups (659.5 μkat/l). This result is different from the previous experiment where the GSH-Px activity increased faster and more significantly after selenite supplementation (Pavlata et al, 2011).…”

“…Not all studies on administration of different forms of Se to goats clearly document a better biological effect of organic Se. For example, Pavlata et al (2011) reported that when comparing supplementation of selenite and a selenium lactate-protein complex there was a similar increase in the blood concentration in goats but in goats supplemented with inorganic Se there was a faster increase in the GSH-Px activity compared to the group supplemented with the lactate-protein complex. This can be explained by the higher and faster biological availability of Se supplied in selenite (for synthesis of seleniumdependent GSH-Px) compared to the Se which is part of the lactate-protein complex.…”

Coagulology, biochemical profile and muscle pathology in calves diagnosed with nutritional muscular dystrophy

“…10 and 19 days after administration of the selenium supplement, respectively. In other studies (Andres et al 1996, Pavlata et al 2011, an increase in GSH-Px activity was observed 10-12 days after selenium supplementation. According to Philipoo et al (1987), GSH-Px activity is not enhanced directly the administration of selenium because selenium is first used to supplement tissue reserves, and it becomes involved in peroxidase synthesis only later.…”

Direct and indirect assessment of selenium status in sheep - a comparison

“…In newborn kids, however, a significant increase in blood Se levels and GSH-Px activity was observed only in the kids of mothers supplemented with sodium selenite. Pavlata et al (2011b) found in an experiment on goats, that the effects of supplementation with selenite and a lactate-protein selenium complex is similar from the standpoint of the ability to supply the organism with selenium, but that the speed of GSH-Px activity increase was better with selenite, which therefore appears to be a biologically better available form of selenium for the production of glutathione peroxidases -functional biologically active selenium proteins. Other authors, for example, Schrauzer (2000), reported that the utilisation of selenomethionine for GSH-Px synthesis also depends on methionine status.…”